Isolation of a novel cyanophage infectious to the filamentous cyanobacterium Planktothrix agardhii (Cyanophyceae) from Lake Donghu, China

Gao, E-Bin; Yuan, Xing; Li, Renhui; Zhang, Qi-Ya

doi:10.3354/ame01266

Cited by 33 publications

(28 citation statements)

References 31 publications

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“…During the past decade, numerous cyanophages have been isolated from freshwater and marine environments. They morphologically belong to three different double-stranded DNA virus families; Myoviridae , Podoviridae and Siphoviridae [30,31,32,33]. On the basis of the sequencing of complete genomes, it has been found that cyanophages contain unique genetic resources [12,13,14,15,16,34,35,36,37,38,39,40,41,42], a vast majority of which are considered as genome database orphans.…”

Section: Introductionmentioning

confidence: 99%

Metabolic Genes within Cyanophage Genomes: Implications for Diversity and Evolution

Gao

Huang

Ning

2016

Genes

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Cyanophages, a group of viruses specifically infecting cyanobacteria, are genetically diverse and extensively abundant in water environments. As a result of selective pressure, cyanophages often acquire a range of metabolic genes from host genomes. The host-derived genes make a significant contribution to the ecological success of cyanophages. In this review, we summarize the host-derived metabolic genes, as well as their origin and roles in cyanophage evolution and important host metabolic pathways, such as the light-dependent reactions of photosynthesis, the pentose phosphate pathway, nutrient acquisition and nucleotide biosynthesis. We also discuss the suitability of the host-derived metabolic genes as potential diagnostic markers for the detection of genetic diversity of cyanophages in natural environments.

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Section: Introductionmentioning

confidence: 99%

Metabolic Genes within Cyanophage Genomes: Implications for Diversity and Evolution

Gao

Huang

Ning

2016

Genes

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“…, 2001; Brown et al. , 2007;Wang and Chen, 2008; Gao et al. , 2009) and have also been confined to experimental studies or small‐scale field investigations (Larsen et al.…”

Section: Introductionmentioning

confidence: 99%

Distribution of microbial populations and their relationship with environmental parameters in the coastal waters of Qingdao, China

Wang¹,

Liang

Bai

et al. 2010

Environmental Microbiology

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In order to understand the large-scale distribution of microbial populations simultaneously and their relationship with environmental parameters, flow cytometry was used to analyse samples collected from 46 stations in the coastal waters of Qingdao in spring, 2007. The distribution of virus was significantly and positively correlated with heterotrophic bacteria. Two groups of picophytoplankton (Synechococcus and picoeukaryotes) were detected; however, Prochlorococcus was not found. Picoeukaryotes and nanophytoplankton were abundant in the near-shore waters, whereas Synechococcus was abundant in the off-shore areas. No variation was found in vertical distribution of virus, heterotrophic bacteria, Synechococcus and nanophytoplankton abundances, except picoeukaryotes abundance in the bottom layer was dramatically lower than that in the upper layers. Correlation analyses indicated that the relationship between abiotic variables and heterotrophic bacteria, pico- and nanophytoplankton was closer than that between abiotic variables and virioplankton. Temperature and nutrients were the synchronous factors controlling the growth of heterotrophic bacteria, pico- and nanophytoplankton in the coastal waters of Qingdao in spring. The results suggested that synergistic and antagonistic effects existed among microbial groups.

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“…The tailed phages are classified into three families: Myoviridae with long contractile tails, Siphoviridae with long noncontractile tails, and Podoviridae with short tails. Various members in each family are morphologically similar and share similar genome organizations, gene contents, and conserved structural protein genes, including capsid protein genes and tail-related genes (21, 23, 26, 37-39, 42, 58).PaV-LD can infect and lyse the filamentous cyanobacterium Planktothrix agardhii (14). To uncover the biological properties and interaction with the host, we sequenced the PaV-LD genome, identified the major structural proteins, and especially analyzed the functional role of a host-like gene that encodes an NblA homologue, which mediates phycobilisome degradation.…”

mentioning

confidence: 99%

“…PaV-LD can infect and lyse the filamentous cyanobacterium Planktothrix agardhii (14). To uncover the biological properties and interaction with the host, we sequenced the PaV-LD genome, identified the major structural proteins, and especially analyzed the functional role of a host-like gene that encodes an NblA homologue, which mediates phycobilisome degradation.…”

mentioning

confidence: 99%

A Novel Cyanophage with a Cyanobacterial Nonbleaching Protein A Gene in the Genome

Gao

Gui

Zhang

2012

J Virol

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A cyanophage, PaV-LD, has been isolated from harmful filamentous cyanobacterium Planktothrix agardhii in Lake Donghu, a shallow freshwater lake in China. Here, we present the cyanophage's genomic organization and major structural proteins. The genome is a 95,299-bp-long, linear double-stranded DNA and contains 142 potential genes. BLAST searches revealed 29 proteins of known function in cyanophages, cyanobacteria, or bacteria. Thirteen major structural proteins ranging in size from 27 kDa to 172 kDa were identified by SDS-PAGE and mass-spectrometric analysis. The genome lacks major genes that are necessary to the tail structure, and the tailless PaV-LD has been confirmed by an electron microscopy comparison with other tail cyanophages and phages. Phylogenetic analysis of the major capsid proteins also reveals an independent branch of PaV-LD that is quite different from other known tail cyanophages and phages. Moreover, the unique genome carries a nonbleaching protein A (NblA) gene (open reading frame [ORF] 022L), which is present in all phycobilisome-containing organisms and mediates phycobilisome degradation. Western blot detection confirmed that 022L was expressed after PaV-LD infection in the host filamentous cyanobacterium. In addition, its appearance was companied by a significant decline of phycocyanobilin content and a color change of the cyanobacterial cells from blue-green to yellow-green. The biological function of PaV-LD nblA was further confirmed by expression in a model cyanobacterium via an integration platform, by spectroscopic analysis and electron microscopy observation. The data indicate that PaV-LD is an exceptional cyanophage of filamentous cyanobacteria, and this novel cyanophage will also provide us with a new vision of the cyanophage-host interactions. Cyanophages are one kind of planktonic viruses that infect cyanobacteria (blue-green algae). Cyanophages and phages have amazing amounts of genetic diversity and biological activity in water environments (33,34,40,54). In either a freshwater or saltwater environment, cyanophages are ubiquitous and play an important role in water ecosystems (46,52,61,66). Generally, the complete genome sequences of cyanophages can provide significant clues for better understanding of the biological properties, ecological effects, and coevolutionary relationships between cyanophages and their hosts (10,17,18,21,27,29). Some cyanophage genomes have been sequenced (32,35,44,49,51,60,64), which has revealed the presence of cyanobacterial genes involved in central energy metabolism and their host's survival. For examples, some photosynthesis-related genes (psbA, hliP, and PSII) and stress-response genes (coding for chaperones and genes associated with bacterial motility and chemotaxis) have been described in cyanophages (4, 5, 31, 36, 47), most of which are transcribed together with essential cyanophage replication-related genes (6, 13, 30, 65). Moreover, a nonbleaching protein A (NblA) gene has been found from a lytic phage, Ma-LMM01, infecting Microcystis aerugi...

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Isolation of a novel cyanophage infectious to the filamentous cyanobacterium Planktothrix agardhii (Cyanophyceae) from Lake Donghu, China

Cited by 33 publications

References 31 publications

Metabolic Genes within Cyanophage Genomes: Implications for Diversity and Evolution

Metabolic Genes within Cyanophage Genomes: Implications for Diversity and Evolution

Distribution of microbial populations and their relationship with environmental parameters in the coastal waters of Qingdao, China

A Novel Cyanophage with a Cyanobacterial Nonbleaching Protein A Gene in the Genome

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