Isolation of a novel deep-sea <i>Bacillus circulus</i> strain and uniform design for optimization of its anti-aflatoxigenic bioactive metabolites production

Zhou, Ying; Wang, Jingying; Gao, Xiujun; Wang, Kai; Wang, Wenwei; Yan, Peisheng

doi:10.1080/21655979.2019.1586055

Cited by 10 publications

(8 citation statements)

References 22 publications

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“…The use of live cells and/or spores offers several additional advantages over the use of metabolites, such as the ability to combine multiple mechanisms, like interference in nematode-host recognition, competition for nutrients, promotion of plant growth, and induction of systemic resistance ( Chinheya et al, 2017 ; Engelbrecht et al, 2018 ; Ramírez-Cariño et al, 2020 ). Additionally, endospores are preferred to prepare commercial formulations because they are highly resistant to adverse environmental conditions, making them suitable for long-term storage and convenient for formulating powdered products for agricultural application ( Zhou et al, 2019 ). In this study, the capacity of B. paralicheniformis TB197 to control PPNs was evaluated for the first time.…”

Section: Discussionmentioning

confidence: 99%

Native Bacillus paralicheniformis isolate as a potential agent for phytopathogenic nematodes control

Chavarria-Quicaño,

Contreras-Jácquez,

Carrillo-Fasio

et al. 2023

Front. Microbiol.

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Phytopathogenic nematodes (PPNs) are responsible for substantial damages within agricultural crops worldwide which can be controlled employing beneficial microorganisms and/or their metabolites in an ecofriendly way. Nevertheless, the success of the control regards not only on the virulence of the strains or the toxicity of their metabolites but also on their ability to colonize and remain in the rhizospheric environment, particularly in those crops affected by abiotic stresses promoted by the climate change. Consequently, the bioprospection of beneficial microorganisms able to control PPNs and to thrive in adverse conditions has attracted attention. On this way, deserts are perfect ecological niches to isolate microorganisms adapted to harsh enviroments. The purpose of this research was to isolate and characterize bacteria from rhizospheric soil samples collected in the Northwestern Desert of Mexico with potential for PPNs control. As first screening, secretomes of each isolate were tested in vitro for nematicidal activity (NA). Then, activities from secretomes and endospores from the selected isolate were confirmed in vivo assays. From 100 thermotolerant isolates, the secretome of the isolate identified as Bacillus paralicheniformis TB197 showed the highest NA (>95%) against Meloidogyne incognita, both in vitro and in vivo tests, suppressing infections caused by M. enterolobii in tomato crops, too. In open field tests, the endospores of TB197 strain showed a reduction of 81% in the infection severity caused by M. enterolobii (p ≤ 0.05), while the galling index (GI) was reduced 84% (p ≤ 0.05) in tomato greenhouse-tests. Also, a reduction of the root necrosis (81%) caused by Radopholus similis in banana plantations (p ≤ 0.05), compared to the control was observed. Owing to their efficacy in controlling PPNs infections, the endospores and secondary metabolites of B. paralicheniformis TB197 strain could be used in bionematicidal formulations.

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Section: Discussionmentioning

confidence: 99%

Native Bacillus paralicheniformis isolate as a potential agent for phytopathogenic nematodes control

Chavarria-Quicaño,

Contreras-Jácquez,

Carrillo-Fasio

et al. 2023

Front. Microbiol.

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“…The fermentation conditions of NSJ105 were optimized using the uniform design method so that the strain NSJ105 could produce the maximum total saponins. The uniform design has the advantage of time and can cover more factors and levels but lacks the superiority of accuracy [ 26 ]. In this study, the concentration of the total saponins measured in the optimal fermentation protocol 105-DP was very close to the predicted value.…”

Section: Discussionmentioning

confidence: 99%

“…It allows researchers to observe variable impacts more accurately and draw precise conclusions. It finds extensive applications in various fields and can improve efficiency and reliability for results [ 24 , 25 , 26 ].…”

Section: Methodsmentioning

confidence: 99%

Optimization of Fermentation Conditions and Product Identification of a Saponin-Producing Endophytic Fungus

Chen,

Wang,

Gao

et al. 2023

Microorganisms

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Background: Some fungal endophytes isolated from P. ginseng may present a new method of obtaining saponins. This experiment aimed to optimize the total saponin yield produced through in vitro fermentation by an endophytic fungus and analyze its saponin species in the fermented extract. Methods: Fermentation protocols were optimized with a uniform design and verified through regression analysis to maximize the total saponin yield. The saponin types under optimal fermentation conditions were then identified and analyzed using Liquid Chromatography–Mass Spectrometry. Results: The Trametes versicolor strain NSJ105 (gene accession number: OR144428) isolated from wild ginseng could produce total saponins. The total saponin yield could be increased more than two-fold through the optimization of fermentation conditions. The concentration of the total saponins achieved by the verified protocol 105-DP was close to the predicted value. The fermentation conditions of the 105-DP protocol were as follows: potato concentration 97.3 mg/mL, glucose concentration 20.6 mg/mL, inoculum volume 2.1%, fermentation broth pH 2.1, fermentation temperature 29.2 °C, and fermentation time 6 d. It was detected and analyzed that the fermented extract of 105-DP contained the ginsenosides Rf and Rb3. Conclusion: The endophytic fungus Trametes versicolor strain NSJ105 has potential application value in saponin production.

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“…However, there might be unknown aflatoxin-control microorganisms in the marine environment, since it has been reported that Emericella venezuelensis , which can produce aflatoxin, originated from the sea [ 39 ]. There has only been one aflatoxin-inhibiting strain from the deep sea reported so far that could inhibit the growth of aflatoxigenic fungi hypha and the generation of AFB 1 [ 40 ]; nevertheless, no strains with AFB 1 degradation properties from the deep sea have been elucidated. In this study, AFB 1 -degrading strain 13 was isolated from the deep sea and identified as Kocuria rosea .…”

Section: Introductionmentioning

confidence: 99%

Isolation and Optimization of Aflatoxin B1 Degradation by Uniform Design and Complete Genome Sequencing of Novel Deep-Sea Kocuria rosea Strain 13

Wang,

Chen,

Yan

et al. 2023

Toxins

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Aflatoxin B1 is a natural carcinogenic mycotoxin. The biological detoxification of aflatoxin could result in less environmental pollution, more moderate conditions, and less impact on food and feed, and be more convenient than physical and chemical methods. In this study, strain 13 with aflatoxin B1 degradation activity (67.47 ± 1.44%) was isolated and identified as Kocuria rosea. A uniform design was applied to optimize the degradation activity using a software Data Processing System, and a quadratic polynomial stepwise regression model was selected to investigate the relationships between the degradation rate and five independent variables. Furthermore, the optimal degradation conditions (culture temperature of 30 °C, culture time of 4.2 days, seawater ratio of 100%, pH of 7.11, and inoculation dosage of 0.09%) were verified with a degradation rate of 88 ± 0.03%, which was well matched with the predicted value (92.97%) of the model. Complete genome sequencing of Kocuria rosea, conducted with a combination of Illumina and single-molecule real-time sequencing, was used to analyze the genomic features and functions of the strain, which were predicted by the annotation based on seven databases, and may provide insights into the potential of Kocuria rosea, as well as providing a reference for degradation gene and protein mining. These results indicate that Kocuria rosea strain 13 has the ability to degrade aflatoxin B1 efficiently, and it also has the potential to provide aflatoxin-degrading enzymes.

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Isolation of a novel deep-sea Bacillus circulus strain and uniform design for optimization of its anti-aflatoxigenic bioactive metabolites production

Cited by 10 publications

References 22 publications

Native Bacillus paralicheniformis isolate as a potential agent for phytopathogenic nematodes control

Native Bacillus paralicheniformis isolate as a potential agent for phytopathogenic nematodes control

Optimization of Fermentation Conditions and Product Identification of a Saponin-Producing Endophytic Fungus

Isolation and Optimization of Aflatoxin B1 Degradation by Uniform Design and Complete Genome Sequencing of Novel Deep-Sea Kocuria rosea Strain 13

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