Isolation of a novel human lung-specific gene,LUNX, a potential molecular marker for detection of micrometastasis in non-small-cell lung cancer

Iwao, Kyoko; Watanabe, Takashi; Fujiwara, Yoshiyuki; Takami, Koji; Kodama, Ken; Higashiyama, Masahiko; H, Yokouchi; Ozaki, Kouichi; Monden, Morito; Tanigami, Akira

doi:10.1002/1097-0215(200002)9999:9999<::aid-ijc1059>3.0.co;2-b

Cited by 88 publications

(84 citation statements)

References 19 publications

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“…Furthermore, as with SFTPB in adenocarcinoma, PVA is superior to TACSTD1 in five of six cases and could, therefore, be useful in a combination marker assay. The last gene included in our secondary screen, LUNX, was first isolated and reported as a novel lung-specific gene of unknown function by Iwao et al (31). Using RT-PCR, LUNX expression was detected in all 35 NSCLC tumor samples and in 80% (16 of 20) of histologically positive lymph nodes.…”

Section: Discussionmentioning

confidence: 97%

A Combination of Molecular Markers Accurately Detects Lymph Node Metastasis in Non–Small Cell Lung Cancer Patients

Xi¹,

Coello²,

Litle³

et al. 2006

Clinical Cancer Research

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Occult lymph node metastasis (micrometastasis) is a good prognostic indicator in non^small cell lung cancer (NSCLC) and could be used to direct adjuvant chemotherapy in stage I patients. This study was designed to evaluate molecular markers for detection of occult lymph node metastasis in NSCLC, define the best marker or marker combination to distinguish positive from benign lymph nodes, and evaluate these markers in lymph nodes from pathologically node-negative (pN 0 ) NSCLC patients. Potential markers were identified through literature and database searches and all markers were analyzed by quantitative reverse transcription-PCR in a primary screen of six NSCLC specimens and 10 benign nodes. Selected markers were further evaluated on 21primary NSCLC specimens, 21positive nodes, and 21benign nodes, and the best individual markers and combinations were identified. A combination of three markers was further validated on an independent set of 32 benign lymph nodes, 38 histologically positive lymph nodes, and 462 lymph nodes from 68 pN 0 NSCLC patients. Forty-two markers were evaluated in the primary screen and eight promising markers were selected for further analysis. A combination of three markers (SFTPB, TACSTD1, and PVA) was identified that provided perfect classification of benign and positive nodes in all sample sets. PVA and SFTPB are particularly powerful in tumors of squamous and adenocarcinoma histologies, respectively, whereas TACSTD1 is a good general marker for NSCLC metastasis.The combination of these genes identified 32 of 462 (7%) lymph nodes from 20 of 68 (29%) patients as potentially positive for occult metastasis. Long-term follow-up will determine the clinical relevance of these findings.

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Section: Discussionmentioning

confidence: 97%

A Combination of Molecular Markers Accurately Detects Lymph Node Metastasis in Non–Small Cell Lung Cancer Patients

Xi¹,

Coello²,

Litle³

et al. 2006

Clinical Cancer Research

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“…Mouse plunc was identified through mRNA differential display methods as a gene upregulated during palate closure of the developing mouse embryo. Similarly, Iwao et al (44) identified a novel human lungspecific gene, LUNX (lung-specific X protein), to be a potential molecular marker for detection of micro-metastasis in nonsmall cell lung cancer. However, the protein properties and function of plunc were not described in these papers.…”

Section: Discussionmentioning

confidence: 99%

Molecular Cloning and Characterization of spurt, a Human Novel Gene That Is Retinoic Acid-inducible and Encodes a Secretory Protein Specific in Upper Respiratory Tracts

Di¹,

Harper²,

Zhao³

et al. 2003

Journal of Biological Chemistry

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Retinoids, such as all-trans-retinoic acid, play an essential role in the regulation of airway epithelial cell growth, differentiation, and gene expression. Using cDNA microarray, we identified a clone, DD4, that contains the cDNA of a novel gene, spurt (secretory protein in upper respiratory tracts) that was significantly induced by all-trans-retinoic acid in primary cultured human tracheobroncheal epithelia. Two alternatively spliced spurt transcripts of 1090 and 1035 base pairs exist that contain the same open reading frame expressing a 256-amino acid peptide. The full-length spurt cDNA sequence spans a genomic DNA fragment of 7,313 bp, and the gene is located on chromosome 20q11.21. spurt mRNA is notably expressed at high levels in human nasal, tracheal, and lung tissues. In situ hybridization demonstrated that spurt message is often present in secretory cell types. The human spurt gene product is a secretory protein that contains a distinct signal peptide sequence in its first 19 amino acids. Mono-specific antibodies were generated to characterize spurt expression. Our data demonstrate that spurt is secreted onto the apical side of primary human airway epithelial cultures and is present in clinical sputum samples. spurt gene expression is higher in sputum and tissue samples obtained from patients with chronic obstructive lung disease. Our results provide the cloning and characterization of this tissue-specific novel gene and its possible relationship with airway diseases.

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“…A number of reports revealed that LunX mRNA is detectable in peripheral blood, pleural fluid and mediastinal lymph nodes from NSCLCs. [4][5][6] Normally, LunX protein expression is relatively low in healthy individuals and only up-regulated in upper respiratory tract upon infection by pathogenic microbes. 7 We recently showed that LunX is overexpressed in the majority of a large panel of NSCLCs and is higher in lymph node metastases.…”

mentioning

confidence: 99%

Lung specific X protein as a novel therapeutic target for lung cancer

Zheng

Tian²,

Wei

2015

OncoImmunology

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Isolation of a novel human lung-specific gene,LUNX, a potential molecular marker for detection of micrometastasis in non-small-cell lung cancer

Cited by 88 publications

References 19 publications

A Combination of Molecular Markers Accurately Detects Lymph Node Metastasis in Non–Small Cell Lung Cancer Patients

A Combination of Molecular Markers Accurately Detects Lymph Node Metastasis in Non–Small Cell Lung Cancer Patients

Molecular Cloning and Characterization of spurt, a Human Novel Gene That Is Retinoic Acid-inducible and Encodes a Secretory Protein Specific in Upper Respiratory Tracts

Lung specific X protein as a novel therapeutic target for lung cancer

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