Isolation of a seed coagulant Moringa oleifera lectin

Santos, Andréa F. S.; Luz, Luciana A.; Argolo, Adriana C. C.; Teixeira, J. A.; Paiva, Patrícia Maria Guedes; Coelho, Luana Cassandra Breitenbach Barroso

doi:10.1016/j.procbio.2009.01.002

Cited by 118 publications

(98 citation statements)

References 35 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…The proteins precipitation was performed by using ammonium sulfate 60% (m/v) for the protein coagulation and precipitation (Andre et al, 2009;Jeso, 1968;Dixon, 1953). The proteins are separated with the supernatant by filtration and dried at room temperature to form fine powder ( Figure 2).…”

Section: Coagulant Bioactive Component Extraction and Precipitationmentioning

confidence: 99%

Combination of alum and extracted <i>Moringa oleifera</i> bioactive molecules powder for municipal wastewater treatment

Kane¹,

Bâ²,

Mahamat³

et al. 2017

Int. J. Bio. Chem. Sci

View full text Add to dashboard Cite

Most of wastewater treatment stations use aluminum sulfate (Al 2 (SO 4 ) 3 , 18H 2 O) and ferric sulfate (Fe 2 (SO 4 ) 3 ) despite their relatively high cost. High turbidity of wastewater constitutes also a limit for a good treatment and increases the cost of the treatment. Moringa oleifera (MO) seeds are well known to have the ability to reduce turbidity and Chemical Oxygen Demand (COD) of wastewater. Moreover, most of the studies focus on using seeds directly for that purpose increasing consequently organic matter content. One of the major objectives of this study is to proposes a contribution for municipal wastewater treatment using crude MO extracted protein powder (MOEP) for the first time for industrial applications. NaCl extraction of MO defatted seeds bioactive molecule is first performed with a yield of 34% and the protein precipitated with ammonium sulfate 60% (m/v) and dried for coagulation as classical protein precipitation. Parameters affecting the effectiveness of MO bioactive protein in wastewater treatment permits to operate at ambient temperature and a range of pH from 6 to 9. The proposed process uses alum and MO bioactive protein molecules powder as coagulant and as additive for classical municipal wastewater treatment. Within a ratio of 50:50 (w/w), more than 90% of the turbidity and 75% of COD. The results obtained with kaolin suspensions showed that MOEP is more efficient for higher turbid wastewater.

show abstract

Section: Coagulant Bioactive Component Extraction and Precipitationmentioning

confidence: 99%

Combination of alum and extracted <i>Moringa oleifera</i> bioactive molecules powder for municipal wastewater treatment

Kane¹,

Bâ²,

Mahamat³

et al. 2017

Int. J. Bio. Chem. Sci

View full text Add to dashboard Cite

show abstract

“…Con dosis de 1.5, 2.0 y 2.5 g L -1 la M. oleifera mostró una reducción de 95 y 92% de turbidez y de color, respectivamente. Estos resultados coinciden con los reportados por Santos et al (2009) y Feria et al (2014, quienes estudiaron la actividad aglutinante de la lectina extraída de diferentes tejidos de la M. oleifera. Igualmente, se observó que las preparaciones de lectina (cMoL) revelaron actividad coagulante superior al 90% y que son proteínas catiónicas divalentes con masa molar de 13 kDa y puntos isoeléctricos entre 10 y 11.…”

Section: Actividad Coagulanteunclassified

Sustancias Naturales: Alternativa para el Tratamiento de Agua del Río Magdalena en Palermo, Colombia

2018

View full text Add to dashboard Cite

* Autor a quien debe ser enviada la correspondenciaRecibido Ago. 29, 2017; Aceptado Nov. 7, 2017; Versión final Dic. 11, 2017, Publicado Jun. 2018 _____________________________________________________________________________________________________________________________________ ResumenEl estudio determinó el capacidad de sustancias naturales de Moringa, Cactus, Neem y Maíz en el tratamiento del agua del río Magdalena que consumen las comunidades del corregimiento de Palermo, en Colombia. Por medio de la prueba de jarras, se establecieron las dosis óptimas de coagulante, tiempo y velocidad de floculación. El sulfato de aluminio (0.02 g L -1 ) se utilizó como control. Los resultados mostraron que Moringa (2g L -1 ) redujo la turbidez en 96.8% y el color en 97.8% mientras que el control logró 95.7% y 96.5%, respectivamente. Por otro lado, el tratamiento con Neem (0.8g L -1 ) disminuyó 99.4% de los coliformes totales y 99.2% de los coliformes fecales mientras que el Maíz (2.5g L -1 ), redujo 94.4% de los coliformes totales. El uso de sustancias naturales es una alternativa asequible y sostenible de potabilización del agua del río Magdalena. Palabras clave: tratamiento de agua; sustancias naturales; Moringa oleifera; Opuntia ficus-indica; Azadirachta indica; Zea mays Natural Substances: Alternative for the Treatment of Magdalena River´s Water in Palermo Colombia AbstractThe study determined the capability of natural substances such as Moringa, Cactus, Neem and Corn on the treatment of water from Magdalena rive, water used by communities in the municipality of Palermo in Colombia. Through jars tests, optimal doses of coagulant, time and flocculation rate were established, aluminum sulfate (0.02 g L -1 ) was used as control. The results showed that Moringa (2g L -1 ) attenuated in 96.8% the turbidity and 97.8% of color while the conventional one obtained 95.7% and 96.5%, respectively; In turn, Neem (0.8g L -1 ) decreased 99.4% total coliforms and 99.2% of fecal coliforms. Also, Corn (2.5g L -1 ), reduced 94.4% of total coliforms. The use of natural substances is a feasible and sustainable alternative to water purification of the Magdalena river.

show abstract

“…Further, naturally occurring cross-linked polysaccharides, which are inexpensive and simple to prepare with epichlorhydrin or divinyl sulphone, have also been extensively used as adsorbent matrices for isolating galactose-specific lectins (Lis & Sharon, 1981). These include guar gum or guaran (Sigma-Aldrich), a galactomannan polysaccharide that binds lectins with anomeric preference for Ǐ-galactose (Alencar et al, 2010;Santos et al, 2009;Souza et al, 2011;Sultan et al, 2009), a polysaccharide isolated from Spondias purpurea (Teixeira et al, 2007), a crosslinked seed gum matrix prepared from plant Leucaena leucocephala (Seshagirirao et al, 2005), a galactomannan from Adenanthera pavonina (Moreira et al, 1998;Teixeira-Sá et al, 2009;Trindade et al, 2006), and a cross-linked galactoxyloglucan from Mucuna sloanei (Teixeira-Sá et al, 2009). Moreover, adsorbent matrices from yeast glucan or curdlan, a polymer of glucose, were prepared to isolate glucose-specific lectins (Mikes & Man, 2003).…”

Section: Adsorbent Matricesmentioning

confidence: 99%