Isolation of a vahlkampfiid amoeba from a contact lens: Tetramitus ovis (Schmidt, 1913) n. comb.

Jonckheere, Johan F. De; Brown, S.

doi:10.1016/j.ejop.2004.11.006

Cited by 12 publications

(14 citation statements)

References 14 publications

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“…Several strains of Vahlkampfia sp., Tetramitus ovis and Paravahlkampfia sp. were isolated from keratitis patients (Aitken et al, 1996;Alexandrakis et al, 1998;De Jonckheere & Brown S., 2005a;Dua et al 1998;Kinnear, 2003;Ozkoc et al, 2008;Walochnik et al, 2000). However, their importance in pathogenesis is unclear and no direct evidence of their pathogenity was indicated.…”

Section: Ecology Of Heteroloboseamentioning

confidence: 99%

Diversity of Heterolobosea

Pánek¹,

Čepička²

2012

Genetic Diversity in Microorganisms

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Section: Ecology Of Heteroloboseamentioning

confidence: 99%

Diversity of Heterolobosea

Pánek¹,

Čepička²

2012

Genetic Diversity in Microorganisms

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“…Except for two species, T. jugosus in the marine environment and T. thermacidophilus which develops at a pH of 1.2-5 and at temperatures up to 54°C, Tetramitus are usually isolated from freshwater with abundant organic matter, as in the CAR where this amoeba was isolated [16–18]. The two strains identified by sequencing, T. entericius and T. waccamawensis are similar to the two species already described [13]. The unidentified strain is also similar to these two species; a further study will be conducted to identify this strain.…”

Section: Discussionmentioning

confidence: 87%

Free-living amoebae isolated in the Central African Republic: epidemiological and molecular aspects

Farra¹,

Bekondi²,

Tricou³

et al. 2017

Pan Afr Med J

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Among the many species of free-living amoebae infecting humans, only Naegleria fowleri, a few species of Acanthamoeba, Balamuthia mandrillaris recently Sappinia diploïdea and Paravahlkampfia Francina are responsible for human diseases especially deadly encephalitis outside of Acanthamoeba keratitis related. In the Central African Republic (CAR), no studies have previously been conducted about free amoebae and no suspicious cases of encephalitis or amoebic keratitis was reported even though the ecosystem supported the proliferation of these microorganisms. The objective of this study was to identify free-living amoebae present in CAR and to define the molecular characteristic. Bathing sites and cerebrospinal fluid from patients died of bacterial meningitis untagged were explored by culture and PCR and the amplicons were sequenced which allowed to characterize the species found. Only species of the genus Tetramitus, namely T. Entericus, T. waccamawensis and T.sp similar to those already described in the world and not pathogenic for humans were found in bathing sites, the cerebrospinal fluid meanwhile remained negative. Although no pathogen species such as Naegleria fowleri or species of Acanthamoeba have been isolated, this study worth pursuing because this investigation was very limited in space because of the insecurity in the country.

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“…). In contrast, no flagellate form has been observed for T. horticolus , T. russelli , T. pararusselli , T. parangularis , T. hohokami , T. anasazii , and T. ovis (De Jonckheere ; De Jonckheere and Brown ; De Jonckheere et al. ; Read et al.…”

Section: Discussionmentioning

confidence: 99%

“…). Tetramitus horticolus and T. ovis have a pink color, cyst pores, and thick endo‐ and ectocyst layers (De Jonckheere and Brown ; De Jonckheere et al. ), whereas isolate MG4 lacks these characteristics.…”

Section: Discussionmentioning

confidence: 99%

“…Assignment of heterolobose amoebae to genus or species by morphology alone is difficult (Brown and De Jonckheere ). However, the 18S rRNA gene and ITS1‐5.8S rRNA gene‐ITS2 sequences are very distinct, and can allow quick identification of new isolates (De Jonckheere and Brown ,b). Unfortunately, the ITS1‐5.8S rRNA gene‐ITS2 sequence might be not useful for new species description because the levels of intraspecific polymorphism within the Heterolobosea is still unknown (Pánek and Čepička ).…”

Section: Discussionmentioning

confidence: 99%

See 1 more Smart Citation

A New Heterolobosean Amoeboflagellate, Tetramitus dokdoensis n. sp., Isolated from a Freshwater Pond on Dokdo Island in the East Sea, Korea

Park

2017

J Eukaryotic Microbiology

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The genus Tetramitus is a representative amoeboflagellate group within the Heterolobosea, and currently contains over a dozen species. Here, a new heterolobosean amoeboflagellate was isolated from a freshwater pond on Dokdo Island, Korea. The amoebae have eruptive pseudopodia, no uroidal filament, and a nucleus with a central nucleolus. The length and width of the amoebae are 15.5-28.0 μm and 5.4-12.6 μm, respectively. The flagellates are conical, with 4 flagella of equal length (~10 μm). There is a discrete rostrum in the subapical region of the flagellate form. The cyst has thin endo- and ectocyst layers and no cyst pores. The amoeba shows slow movement at 37 °C, but does not move at 42 °C under a light microscope. Phylogenies of the 18S rRNA gene and the ITS1-5.8S rRNA gene-ITS2 sequence show that the strain belongs to a subclade of Tetramitus that includes Tetramitus rostratus, Tetramitus waccamawensis and Tetramitus entericus, amongst others. Nonetheless, the strain is distinct from other species in both molecular phylogenetic trees. Thus the strain isolated from the Dokdo Island is proposed as a novel species, Tetramitus dokdoensis n. sp.

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Isolation of a vahlkampfiid amoeba from a contact lens: Tetramitus ovis (Schmidt, 1913) n. comb.

Cited by 12 publications

References 14 publications

Diversity of Heterolobosea

Diversity of Heterolobosea

Free-living amoebae isolated in the Central African Republic: epidemiological and molecular aspects

A New Heterolobosean Amoeboflagellate, Tetramitus dokdoensis n. sp., Isolated from a Freshwater Pond on Dokdo Island in the East Sea, Korea

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