Isolation of adenylate-rich RNA from Physarum polycephalum

Characterization of polyadenylate from the fungus Trichoderma viride

“…Differences between poly(A) sedimentation and electrophoretic patterns relative to specific marker RNAs are not unexpected (cf. 19) and have been reported by other investigators (5,17,19). Results similar to those shown in Fig.…”

“…The biological significance of this size variation is not yet clear. Although short segments have been found attached to a few specific messenger RNAs in certain higher organisms (7, 9), generally speaking, the average nucleotide length of the poly(A) population within an organism seems to increase with increasing genome complexity (5,20).…”

The Effect of Age on the Properties of Poly(A)-containing Messenger RNA in Physarum polycephalum

“…However, it was clear that the distribution of lengths for the poly(A) segments in poly(A)+ mRNA was in the range 140 to 220 nucleotides. These results are consistent with those of Fouquet et al (1974), but disagree with the average length of 65 nucleotides reported by Adams & Jeffery (1978). The reason for these discrepancies is not known, though it is likely to reside in differences in the conditions of digestion with ribonucleases.…”

Poly(adenylic acid) degradation by two distinct processes in the cytoplasmic RNA of Physarum polycephalum

“…Poly(A) in Nuclear and Cytoplasmic RNA. Figure 1 illustrates the electrophoretic mobility of the RNase A and T] resistant polynucleotides present in Physarum high molecular weight nuclear and cytoplasmic RNA (Fouquet et al, 1974). In most of these poly(acrylamide) gels, two distinct, although quite heterogeneous, nucleotide classes are evident.…”