Isolation of Agrobacterium tumefaciens Strains from Crown Gall Sample of Dicot Plants in Bangladesh

Islam, Soriful; Akter, MY; Rahman, Md. Atikur; Rahman, M. M.; Akhtar, Muhammad; Alam, Mohammad Firoz

doi:10.3923/crb.2010.27.36

Cited by 12 publications

(6 citation statements)

References 9 publications

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“…It was chosen because a distinct colony could be formed on this medium, but not on other media (Davoodi et al, 2013). As characteristics of colony formed on selective medium matched with colony morphologies reported previously (Atlas, 2010;Clark, 1969;Islam et al, 2010;Kado and Heskett, 1970), strains isolated from roses with crown gall disease were confirmed to be A. tumefaciens.…”

Section: Resultssupporting

confidence: 83%

“…A. tumefaciens can produce opines and agrocinopines and, use them as carbon and energy sources (Ellis and Murphy, 1981). In addition, not all A. tumefaciens strains isolated from individuals infected with crown gall were identical in pathogenicity (Islam et al, 2010;Xu et al, 2011). A. tumefaciens RC12 isolate was chosen as an inoculation strain because it was positive for agrocinopine by PCR analysis and identified as a pathogenic A. tumefaciens (Chandrasekaran et al, 2019).…”

Section: Resultsmentioning

confidence: 99%

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Cultivar Resistance of Korean Breeding Cut-Rose against Crown Gall by Agrobacterium tumefaciens Evaluated by an In Vitro Inoculation

2023

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Rose crown gall caused by Agrobacterium tumefaciens is a major disease that damages the production of cut-roses in Korea. The effective prevention methods for this disease include the use of resistant varieties. This study was conducted to evaluate the resistance of 58 Korean cultivars and six foreign cultivars to crown gall disease with nodal explants in vitro. Among 180 A. tumefaciens strains, pathogenic strain RC12 was selected as an inoculant strain. The strain RC12 was identified based on characteristics of some selective media, pathogenicity test, and polymerase chain reaction analysis. Forty rose cultivars formed tumors on explants inoculated with A. tumefaciens RC12. However, 24 cultivars, including 22 Korean cultivars and 2 foreign cultivars, showed resistance to A. tumefaciens RC12 without forming any tumors. Six cultivars with tumor formation rates of over 30% formed initial tumors within 23 days after inoculation. Six cultivars with low tumor formation rates of around 5% formed initial tumors after 28 days of inoculation. It was found that gall formation rate was highly correlated with the initial gall formation period. Thus, the relationship between the period of gall formation and the rate of gall formation could be useful for assessing resistance to crown gall disease. In vitro inoculation methods could be used to evaluate resistance of cut-rose cultivars to crown gall diseases.

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Section: Resultssupporting

confidence: 83%

Section: Resultsmentioning

confidence: 99%

Cultivar Resistance of Korean Breeding Cut-Rose against Crown Gall by Agrobacterium tumefaciens Evaluated by an In Vitro Inoculation

2023

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“…The isolates were gram negative rods, motile, catalase positive, urease positive, citrate positive and exhibited negative reaction to both indole and hydrogen sulfide tests. These properties were in agreement with those reported by Islam (2010), for physical and biochemical characteristics of A.tumefaciens. Unlike most bacteria, Agrobacterium tumefaciens is known to produce ketolactose enzyme which causes precipitation of cuprous oxide in Benedict's reagent found in lactose broth, differentiating the isolates from Rhizobium species (Aysan and Sahin, 2003).…”

Section: Isolation and Characterization Of Agrobacterium Tumefacienssupporting

confidence: 92%

Antibacterial effect of Artemisia and ginger extracts in controlling Agrobacterium tumefaciens in roses

Njagi

Nyamwange²,

Njeru

et al. 2021

JFCLS

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Rose is the world’s most traded cut flowers with 74 % being produced in Kenya. Pests like spider mites, caterpillar, aphids, thrips, nematodes and diseases such as crown gall, downy mildew, powdery mildew and botrytis highly compromise rose production. Crown gall disease caused by Agrobacterium tumefaciens is the most problematic disease of roses in Kenya, causing a production loss of up to 60 % depending on the age and variety of rose. An experiment to determine the antibacterial effect of Artemisia and ginger extracts was carried out in vitro, where the inhibitory zones around filter discs soaked with extracts on Muller Hinton agar was established. The extracts were emulsified with dimethylsulfoxide (50 %) and minimum inhibitory concentrations of Artemisia (125 mg/ml), ginger (62.5 mg/ml) and mixture of Artemisia and ginger (31.25 mg/ml) were used to soak the filter discs whereas the commercial recommended rate of copper hydroxide of 6.25 mg/ml was used. Artemisia and copper hydroxide (commercial antibiotic) had highest inhibition zone of 12.80 mm compared to ginger 10.60 mm. A mixture of Artemisia and Ginger had a slightly lower inhibition zone (10.20 mm) though not significantly different from ginger (P> 0.001). An eight-month greenhouse experiment was also done to determine the efficacy of the extracts on inoculated rose plants. The results showed that crown gall incidence and gall weight were low but not significantly different from copper hydroxide and Artemisia. Crop vigor, which was indicated by stem length was highest for Artemisia treatment with an average of 73.54 cm followed by copper hydroxide (67.25 cm) while ginger and mixture of ginger and Artemisia had 53.44 cm and 64.70 cm respectively. From the results of this research, Artemisia and Ginger extracts are promising alternative to control crown gall and possibly other diseases in field crops. Artemisia performance compares well with copper hydroxide and therefore the best alternative to replace copper hydroxide.

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“…Loopful of sample was taken from the serially diluted culture tubes and inoculated into the prepared YEM/MacConkey plates and kept for incubation at 27 ± 1° C for 2 days. Then, the isolated single colonies were maintained at Mannitol agar slants and were stored in refrigerator at 4°C for further use (Islam et al, 2010).…”

Section: Isolation Of Agrobacterium Tumefaciens From Gall Samplementioning

confidence: 99%

Mutagenesis Induced Improvement of Coenzyme Q10 Production by Agrobacterium tumefaciens

Singh

Akhter

2022

J. Res. Appl. Sci. Biotechnol.

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Mutagenesis induced improvement of coenzyme Q10 production by Agrobacterium tumefaciens with the view of increasing CoQ10 production via strain development by UV and EMS mutagenesis is of great importance. Further optimisation of fermentation parameters such as pH, temperature, and inoculum load was done to maximise CoQ10 yield. Soil and gall samples were collected from vegetable field of Research farm located at Chatha, Jammu. Repeated isolations and purification resulted in 05 Agrobacterium tumefaciens cultures from soil samples and 07 from that of gall samples. Identification of cultures was confirmed by morphological studies. Agrobacterium tumefaciens cultures were grown on selective media to screen CoQ10 producing strains. G12 was best amongst 12 isolates; it produced 2.36 mg/g DCW. Mutagenesis using UV and EMS treatment was done to obtain a high-CoQ10-producing strain from the native isolate (G12). When exposed for 7 minutes to mutagenic UV radiation from a distance of 10 cm, the native strain (G12) showed reduction in number of colonies. Also the viability of cells was reduced when they were treated with 80 μM EMS for 30 min but it was less effective than UV mutagenesis. G12 UV mutant strain was selected after screening and tested for CoQ10 production potential by flask culturing. Our results indicate that CoQ10 content increased from 2.36 mg/g DCW to 4.34 mg/ g DCW after mutagenesis, indicating positive mutations. G12 UV mutant was further studied in batch cultures with different inoculum loads at various temperatures and range of pH, to maximize CoQ10 production along with constant fermentation parameters like agitation (180 rpm) and incubation time (96 hours). Therefore, this study suggests that amongst the culture conditions tested so far for CoQ10 production, G12 mutant strain showed maximum CoQ10 content when 108 CFU/ml of inoculum load is used at 32°C and pH 7.0.

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Isolation of Agrobacterium tumefaciens Strains from Crown Gall Sample of Dicot Plants in Bangladesh

Cited by 12 publications

References 9 publications

Cultivar Resistance of Korean Breeding Cut-Rose against Crown Gall by Agrobacterium tumefaciens Evaluated by an In Vitro Inoculation

Cultivar Resistance of Korean Breeding Cut-Rose against Crown Gall by Agrobacterium tumefaciens Evaluated by an In Vitro Inoculation

Antibacterial effect of Artemisia and ginger extracts in controlling Agrobacterium tumefaciens in roses

Mutagenesis Induced Improvement of Coenzyme Q10 Production by Agrobacterium tumefaciens

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