Isolation of Amantadine-Resistant Influenza A Viruses (H3N2) from Patients following Administration of Amantadine in Japan

Iwahashi, Jun; Tsuji, Katsuro; Ishibashi, Tetsuya; Kajiwara, Junboku; Imamura, Yoshihiro; Mori, Ryoichi; Hara, Koyu; Kashiwagi, Takanari; Ohtsu, Yasushi; Hamada, Nobuyuki; Maeda, Hideo; Toyoda, Michiko; Toyoda, Tetsuya

doi:10.1128/jcm.39.4.000-000.2001

Cited by 14 publications

(10 citation statements)

References 17 publications

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“…However, it should be noted that chloroquine and hydroxychloroquine overdoses (although not common) do occur with a certain frequency and can result in significantly higher levels of drug (ϳ30 M) in the blood of these patients (19,22). Amantadine has been used as a therapy for influenza virus A infection (18,34). However, its use has diminished due to the fact that resistant strains emerge both in the laboratory and in patients.…”

Section: Discussionmentioning

confidence: 99%

Inhibition of Endosomal/Lysosomal Degradation Increases the Infectivity of Human Immunodeficiency Virus

Fredericksen

Wei

Yao

et al. 2002

J Virol

192

165

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Section: Discussionmentioning

confidence: 99%

Inhibition of Endosomal/Lysosomal Degradation Increases the Infectivity of Human Immunodeficiency Virus

Fredericksen

Wei

Yao

et al. 2002

J Virol

192

165

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“…The two classes of anti-influenza A virus compounds available currently are the ion channel blockers (amantadine or rimantadine) and the neuraminidase inhibitors (oseltamivir or zanamivir). It is well documented that amantadine-or rimantadine-resistant viruses are capable of causing disease and spreading person-to-person (Hayden and Hay, 1992;Iwahashi et al, 2001;Shiraishi et al, 2003). A recent study suggests that oseltamivir-resistant viruses can develop in humans, and patients harboring oseltamivir-resistant viruses can shed virus for significant amounts of time (Kiso et al, 2004).…”

Section: Discussionmentioning

confidence: 99%

Virus-inducible reporter genes as a tool for detecting and quantifying influenza A virus replication

Lutz

Dyall

Olivo

et al. 2005

Journal of Virological Methods

103

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SummaryThe use of influenza A virus-inducible reporter gene segments in detecting influenza A virus replication was investigated. The RNA polymerase I promoter/terminator cassette was used to express RNA transcripts encoding green fluorescence protein or firefly luciferase flanked by the untranslated regions of the influenza A/WSN/33 NP segment. Reporter gene activity was detected after reconstitution of the influenza A virus polymerase complex from cDNA or after virus infection, and was influenza A virus-specific. Reporter gene activity could be detected as early as 6 hours post infection and was virus dose-dependent. Inhibitory effects of antibodies or amantadine could be detected a nd quantified rapidly, providing a means of not only identifying influenza A virus -specific replication, but of determining the antigenic subtype as well as antiviral drug susceptibility. Induction of virus-specific reporter genes provides a rapid, sensitive method for detecting virus replication, quantifying virus titers and assessing antiviral sensitivity as well as antigenic subtype.

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“…There have been several previous reports about the emergence of resistance to the anti-influenza adamantane (amantadane and rimantadine) drugs in seasonal human influenza A(H3N2) viruses, particularly from Japan where anti-influenza drugs are used widely [Iwahashi et al, 2001;Saito et al, 2002Saito et al, , 2003Saito et al, , 2006Saito et al, , 2007Suzuki et al, 2003;Kitahori et al, 2006;Yoneda et al, 2007], from the USA [Bright et al, 2006], and worldwide [Ziegler et al, 1999;Bright et al, 2005]. Bright et al found that out of 7000 influenza A isolates collected between 1994 and 2005, the frequency of adamantane resistance-associated mutations rose from 0.4% in 19940.4% in -19950.4% in to 12.3% in 20030.4% in -20040.4% in [Bright et al, 2005.…”

Section: Introductionmentioning

confidence: 99%

Emergence of adamantane‐resistant influenza A(H3N2) viruses in Hong Kong between 1997 and 2006

Tang

Ngai

Wong

et al. 2008

Journal of Medical Virology

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Resistance to adamantanes for treating influenza A(H3N2) has increased dramatically, worldwide, over the past 5 years. A comprehensive 10-year data set on the rise of adamantane-resistance in Hong Kong is reported. Nucleotide sequences encoding the M2-ion channel of influenza A(H3N2) were obtained from 281 H3N2 isolates collected from adamantane-naive children admitted to a teaching hospital in Hong Kong, between 1997 and 2006. These sequences were screened for the presence of recognized adamantane resistance-associated amino acid mutations (L26F, V27A, A30T, S31N, G34E). The amantadane (rimantadine is not used in Hong Kong) usage for this hospital during this period was also collated. Fifty-eight isolates harbored at least one of these resistance signatures, the majority (57/58) being S31N, which increased rapidly over 2003-2005 from 20% to 83.3%. Other amino acid mutations, not previously associated with adamantane resistance, were also found. In particular, I51V was found to rise in frequency, along with S31N, though the significance of this mutation remains uncertain at present. A rise in amantadane usage occurred in Hong Kong between 2000 and 2002, which slightly preceded the subsequent rise in adamantane-resistant influenza A(H3N2) isolates. This study shows a temporal correlation between increases in amantadane prescriptions and the prevalence of adamantane-resistant influenza A(H3N2) viruses in Hong Kong. The underlying reasons for these findings are unclear and similar studies are required elsewhere to elucidate these and prevent this spread of drug-resistance happening again in future.

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Isolation of Amantadine-Resistant Influenza A Viruses (H3N2) from Patients following Administration of Amantadine in Japan

Cited by 14 publications

References 17 publications

Inhibition of Endosomal/Lysosomal Degradation Increases the Infectivity of Human Immunodeficiency Virus

Inhibition of Endosomal/Lysosomal Degradation Increases the Infectivity of Human Immunodeficiency Virus

Virus-inducible reporter genes as a tool for detecting and quantifying influenza A virus replication

Emergence of adamantane‐resistant influenza A(H3N2) viruses in Hong Kong between 1997 and 2006

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