Isolation of an Aerobic Denitrifying Bacterial Strain from a Biofilter for Removal of Nitrogen Oxide

Abstract

A strain of bacteria CP1 with high nitrogen removal efficiency was newly isolated from the biofilm of a biofilter for removal of NO x from flue gas. The isolate was identified as Pseudomonas aeruginosa based on its physiological and biochemical characteristics and the results of 16S rRNA gene homology analysis. The new isolate had a high denitrifying ability, removing 98.49% of the nitrate in a 24-h period under aerobic conditions, with no nitrite accumulation. With regard to the nitrogen balance, the percentage of nitrogen lost in the flask culture was estimated to be 32.3%, which was presumed to be converted to nitrogen gas. An analysis of its denitrification activity showed that the optimal C/N and temperature were 12 and 30°C-40°C, respectively. By using glucose, sodium citrate and succinate, CP1 removed nitrate with high denitrification efficiency. The change in DO did not influence the effects of denitrification when it varied from 0 to 7.2 mg/L. The results show that CP1 could be a good candidate for the process of aerobic denitrification.

Nitrogen-removal efficiency of a novel aerobic denitrifying bacterium, Pseudomonas stutzeri strain ZF31, isolated from a drinking-water reservoir

“…For instance, Wang et al (2013) investigated the effect of various carbon sources, temperatures, and pH on the denitrification performance of the aerobic denitrifying bacterium strain HS-N62. Wu et al (2013) determined the optimal C/N ratio, temperature, and dissolved oxygen parameters for the denitrification activity of the aerobic denitrifier strain CP1. Most previous studies have primarily been single-factor experiments, but simultaneous changes in multiple environmental factors may impact nitrogen removal efficiency (Su et al, 2015).…”

“…Several studies have focused on nutritional and physical factors that regulate the nitrogen-removal characteristics of aerobic denitrifying bacteria (Zhang et al, 2012;Wu et al, 2013). For instance, Wang et al (2013) investigated the effect of various carbon sources, temperatures, and pH on the denitrification performance of the aerobic denitrifying bacterium strain HS-N62.…”

NAP enzyme recruitment in simultaneous bioremediation and nanoparticles synthesis

“…The beads entrapped with MTT-cells / NAP-enzyme were applied in sterile distilled water artificially inoculated with 3 mM of AgNO 3 for 288 h (12 days). The concentrations of NO 3 − and NO 2 − were determined according to [ 32 ] every 12 h interval [ 33 , 34 ]. For assuring AgNPs synthesis by both systems, the immobilized beads were examined SEM, EDX and XRD at the end of the experiment.…”

Complete genome of a denitrifying Halioglobus sp. RR3-57 isolated from a seawater recirculating aquaculture system

“…For identification of denitrifying bacteria, bacteria were isolated from biofilters in a seawater RAS operated in National Institute of Fisheries Science (NIFS), Busan, South Korea (Lee et al, 2016). Bacterial strains were cultivated on a modified denitrification medium (Wu et al, 2013) at 25°C for 7 days. Among isolated strains, a Gram-negative, aerobic bacterium, the strain RR3-57 showed 96.2% of 16S rRNA gene similarity with encoded 37 predicted nitrogen metabolism-related genes and among them, 18 genes were associated with denitrification, such as respiratory nitrate reductase, cytochrome cd1 nitrite reductase and nitricoxide reductase which reduce nitrate to nitrite, nitrite to nitric oxide and nitric oxide to dinitrogen oxide sequentially.…”