Isolation of an ampicillin-resistant, non-beta-lactamase-producing strain of Haemophilus influenzae

Markowitz, Sheldon M.

doi:10.1128/aac.17.1.80

Cited by 109 publications

(57 citation statements)

References 9 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…BLNAR isolates of NTHi were first reported in 1980 [19] and generally continue to be isolated at low frequencies in Western Europe and the USA [20][21][22][23] . However, recent surveillance studies have reported higher proportions of BLNAR isolates [10,11,17,24,25] .…”

Section: Discussionmentioning

confidence: 99%

Clonal spread of β-lactamase-producing amoxicillin–clavulanate-resistant (BLPACR) strains of non-typeable Haemophilus influenzae among young children attending a day care in Japan

Ito

Hotomi

Maruyama

et al. 2010

International Journal of Pediatric Otorhinolaryngology

View full text Add to dashboard Cite

Objective: Resistant strains of non-typeable Haemophilus influenzae (NTHi) are one of the principal causes of recurrent acute otitis media (otitis prone), rhinosinusitis, and pneumonia in young children. β-lactamase-nonproducing ampicillin-resistant (BLNAR) strains are particularly common in Japan, and β-lactamase-producing amoxicillin-clavulanate resistant (BLPACR) strains are now emerging. We investigated the nasopharyngeal carriage status of these resistant strains among children attending a same day care center during a 10-year period. Methods:From 1999 to 2008, we obtained nasopharyngeal swab specimens from young children attending a same day care center and examined the incidence of resistant strains of NTHi.Antimicrobial resistance of NTHi was identified based on PCR analysis of mutation of the penicillin binding protein (PBP) genes. Pulsed-field gel electrophoresis (PFGE) was performed to examine the clonal relationship of each resistant strain. Results:The prevalence of resistant strains of NTHi among the children attending this day care has significantly increased during the past 10 years and most of this day care children recently have resistant strains with PBP gene mutations in their nasopharynx. Genetically BLPACR (gBLPACR) strains have rapidly increased since 2007 and PFGE analysis demonstrated that all gBLPACR were clonally identical. This is the first report of apparent clonal dissemination of gBLPACR strains of NTHi occurring in a certain environment such as day care. Conclusions:The rapidly increasing prevalence of resistant strains, in particular gBLPACR, in this day care center may predict a high incidence of these resistant bacteria from clinical isolates in the near future and potential serious medical problems worldwide.

show abstract

Section: Discussionmentioning

confidence: 99%

Clonal spread of β-lactamase-producing amoxicillin–clavulanate-resistant (BLPACR) strains of non-typeable Haemophilus influenzae among young children attending a day care in Japan

Ito

Hotomi

Maruyama

et al. 2010

International Journal of Pediatric Otorhinolaryngology

View full text Add to dashboard Cite

show abstract

“…The major mechanism of this resistance was the production of beta-lactamases [7]. Non-beta-lactamase-mediated resistance to ampicillin in Haemophilus influenzae was first reported in the early 1980s [8]. This beta-lactamase-negative ampicillin-resistant (BLNAR) determinant was associated with the alteration of penicillin-binding proteins (PBPs) as a result of ftsI gene mutation [9] [10].…”

Section: Introductionmentioning

confidence: 99%

Clinical Characteristics of Haemophilus influenzae at General Hospital in the Central Region of Japan

Minami¹,

Sakakibara²,

Imura³

et al. 2016

JBM

View full text Add to dashboard Cite

Haemophilus influenzae is an important pathogen that caused several infection diseases, such as sinusitis, otitis media, sepsis, and meningitis. This study was conducted to find out the prevalence and antimicrobial susceptibility pattern of Haemophilus influenzae isolates at general hospital in the central region of Japan from December 2015 to January 2016. Haemophilus influenzae was identified by standard laboratory procedure. Antimicrobial susceptibility testing was performed by micro dilution assay according to CLSI recommendation. One hundred ninety-one Haemophilus influenzae were isolated, among which 95 (49.7%) were from male and 96 (50.3%) were from female. The age incidence of (0) years, (≤2) years, (≤5) years, and (6≤) years groups were 22(11.5%), 92(48.2%), 61(31.9%), and 16(8.4%), respectively. Positive samples were received mostly from the nasal discharge (177/92.7%), sputum (6/3.1%), tonsillar (6/3.1%), and pharynx (2/1.0%). Ceftriaxone was the most active antibiotics with 100% susceptible rates, followed by ciprofloxacin (99.5%) and minocycline (99%) in our study. Furthermore, we categorized four patterns: beta lactamase-negative ampicillin-sensitive strain (BLNAS), beta lactamase-negative ampicillin-resistant strain (BLNAR), beta lactamase-positive ampicillin resistant strain (BLPAR), and beta lactamase-positive amoxicillin-clavulanic acid-resistant strain (BLPACR) from those ampicillin susceptible results. The numbers of female were significant greater than those of male in BLPAR (p = 0.0336). With respect to antimicrobial susceptible pattern, there was no minocycline and piperacillin resistant strain in both BLNAS and BLNAR (p < 0.0001). Haemophilus influenzae infection spreads worldwide and inadequate use of antibiotics contributes to uptake of their new antimicrobial resistance. Continuous antimicrobial surveys are need for control the emergence and spread of antimicrobial resistance to reduce the morbidity and mortality.

show abstract

“…At the same time there is some evidence that the resistance does not necessarily correlate with the enzyme activity (8,12,17). The existence of mutants resistant to new p-lactam antibiotics which are highly resistant to hydrolysis by p-lactamases (3,5,14) led us to consider the possibility that they might contain resistance mechanisms other than p-lactamase production.…”

Section: Distribution Of R Plasmids Conferring a Permeabilitymentioning

confidence: 99%

Distribution of R Plasmids Conferring a Permeability Barrier to Cephalosporins on Bacteria Isolated from Clinical Specimens

Ikeuchi

Osada

1981

Microbiology and Immunology

View full text Add to dashboard Cite

Isolation of an ampicillin-resistant, non-beta-lactamase-producing strain of Haemophilus influenzae

Cited by 109 publications

References 9 publications

Clonal spread of β-lactamase-producing amoxicillin–clavulanate-resistant (BLPACR) strains of non-typeable Haemophilus influenzae among young children attending a day care in Japan

Clonal spread of β-lactamase-producing amoxicillin–clavulanate-resistant (BLPACR) strains of non-typeable Haemophilus influenzae among young children attending a day care in Japan

Clinical Characteristics of Haemophilus influenzae at General Hospital in the Central Region of Japan

Distribution of R Plasmids Conferring a Permeability Barrier to Cephalosporins on Bacteria Isolated from Clinical Specimens

Contact Info

Product

Resources

About