1997
DOI: 10.1128/mcb.17.6.3094
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Isolation of an AP-1 Repressor by a Novel Method for Detecting Protein-Protein Interactions

Abstract: Transcription factor AP-1 transduces environmental signals to the transcriptional machinery. To ensure a quick response yet maintain tight control over AP-1 target genes, AP-1 activity is likely to be negatively regulated in nonstimulated cells. To identify proteins that interact with the Jun subunits of AP-1 and repress its activity, we developed a novel screen for detecting protein-protein interactions that is not based on a transcriptional readout. In this system, the mammalian guanyl nucleotide exchange fa… Show more

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Cited by 442 publications
(458 citation statements)
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References 40 publications
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“…Jun-dimerization protein (JDP2), another AP-1 protein, is a dimerization partner of Jun [3] and ATF2 [4] and represses transcriptional activation. JDP2 has effects on various cellular processes.…”
Section: Introductionmentioning
confidence: 99%
“…Jun-dimerization protein (JDP2), another AP-1 protein, is a dimerization partner of Jun [3] and ATF2 [4] and represses transcriptional activation. JDP2 has effects on various cellular processes.…”
Section: Introductionmentioning
confidence: 99%
“…12,13 Jun dimerization protein 2 (JDP2) is a DNA-binding protein that forms homodimers or heterodimers with c-Jun, ATF2 and C/EBPg. [14][15][16] JDP2 can function not only as a transcriptional repressor but also as a coactivator in various types of cell, 15,[17][18][19][20] and it is involved in a variety of biological phenomena such as proliferation and differentiation of cells and apoptosis. 14,15,17,18,[20][21][22] For example, forced expression of JDP2 represses the retinoic acid-mediated (RA-mediated) transcription of the c-jun gene and the differentiation of F9 cells in response to RA.…”
mentioning
confidence: 99%
“…Recently, two novel protein-protein interaction assays were developed which complement and overcome some of the problems and limitations of the two hybrid system (11,12). These assays are based on a completely different readout to monitor a successful protein-protein interaction in yeast.…”
Section: Introductionmentioning
confidence: 99%