Isolation of an Encapsulated Strain of <i>Staphylococcus epidermidis</i>

Yoshida, Kenichi; Minegishi, Yoshihisa

doi:10.1111/j.1365-2672.1979.tb01758.x

Cited by 34 publications

(13 citation statements)

References 8 publications

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“…Little is known about the localization and distribution of surface components which mediate bacterial adhesion to hydrocarbons (Rosenberg, 1984). Yoshida & Minegishi (1979) reported that an encapsulated S . epidermidis strain had proteincontaining spike-like structures protruding from the cell wall.…”

Section: Discussionmentioning

confidence: 99%

Adhesion of Staphylococcus epidermidis and Staphylococcus saprophyticus to a Hydrophobic Biomaterial

Hogt

Dankert²,

Feijén

1985

Microbiology

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~The relative surface charge and hydrophobicity of 16 strains of Staphylococcus epidermidis showed large variations. For this species no relationship between the two surface parameters was found. A highly negative surface charge was observed in all seven encapsulated strains (one S. epidermidis and six Staphylococcus saprophyticus strains). The adhesion of the staphylococci to fluorinated polyethylene-propylene films was not related to the relative surface charge and the hydrophobicity of the bacteria. On films pre-exposed to human plasma, the bacterial adhesion was substantially reduced. Mechanisms involved in the adhesion of coagulase-negative staphylococci to this biomaterial are discussed.

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Section: Discussionmentioning

confidence: 99%

Adhesion of Staphylococcus epidermidis and Staphylococcus saprophyticus to a Hydrophobic Biomaterial

Hogt

Dankert²,

Feijén

1985

Microbiology

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“…hyicus, well-defined antiphagocytic capsule was observed in a strain by electron microscopy (Yoshida et al 1988a) as in Staph. aureus (Yoshida & Minegishi 1979) and Staph. epidermidis (Ichiman & Yoshida 1981), and an encapsulated strain was examined in our experiments to compare the immunological properties of three capsular type strains of Staph.…”

Section: Discussionmentioning

confidence: 99%

Cross protection between an encapsulated strain of Staphylococcus hyicus and encapsulated strains of Staphylococcus aureus

Yoshida

Ichiman

Umeda

1988

Journal of Applied Bacteriology

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Strain ST67P of Staphylococcus hyicus grew diffusely in regular serum-soft agar. With the addition of rabbit antisera prepared with Staph. aureus strains, Smith, NS58D or NS41D, capsular type A, B or C, respectively, the organisms converted to compact type growth. Mice immunized with heat-killed vaccine of strain ST67P showed significant resistance against either homologous or heterologous strains, Smith, NS58D and NS41D. Passive protective activities in rabbit antisera prepared with strains Smith, NS58D and NS41D were absorbed out with either homologous cell surface polysaccharide fraction or cell surface polysaccharide fraction extracted from strain ST67P. Well-defined large capsules were observed in ultra-thin sections treated with rabbit antiserum prepared with homologous strain conjugated with ferritin. Also, the capsule surrounded by ferritin granules was shown in ultra-thin sections treated with ferritin conjugated with antisera prepared with those heterologous strains although the capsular size was significantly smaller than those observed by homologous antiserum.

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“…Staphylococcal slime is water-soluble and can be removed from the bacterial surface by washing with water [60,61]. Growth on media solidified with agar, preferably under liquid, was at first recommended for the production of extracellular staphylococcal slime, perhaps because the slimy appearance of the bacteria is then very obvious [62,63].…”

Section: Studies With Complex Media and The Use Of Agar As A Gelling mentioning

confidence: 99%

“…Glycerol phosphate was also present in significant amounts and glucose and glucuronic acid were also detected. Seltmann and Beer [61] isolated extracellular material (which they termed 'capsular material') using the method of Ekstedt and Bernhard [60]. The isolated material gave 13 peaks on elution from DEAE-cellulose, all except one being rich in galactose.…”

Section: Studies With Complex Media and The Use Of Agar As A Gelling mentioning

confidence: 99%

The slime of coagulase-negative staphylococci: Biochemistry and relation to adherence

Hussain

Wilcox

White

1993

FEMS Microbiology Letters

128

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In recent years, infections of implanted plastic devices by coagulase‐negative staphylococci have become a major cause of septicaemia in human patients. The causal bacterial species is usually Staphylococcus epidermidis and these organisms grow as a biofilm adherent to a solid surface. Several methods have been introduced to assess the mass of adherent bacteria and the slimy matrix in which they are embedded. Some methods measure total biofilm, others measure the organisms or the slime alone. In vitro, the type of medium, the atmosphere during incubation, and the nature of the solid surface, affect the quantity of biofilm that is formed. In most studies on the chemistry of the slime, the material used was formed on complex media solidified with agar. Contamination by ingredients of the media or by agar may not always have been recognised. Recent work with chemically defined medium (liquid or solidified with silica gel) shows that the slime is a mixture of about 80% (w/w) teichoic acid and 20% protein. Growth as a biofilm may protect the staphylococci from antibiotics. At present, the greatest success in preventing infection has come from improved surgical techniques during the insertion of implants.

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Isolation of an Encapsulated Strain of Staphylococcus epidermidis

Abstract: An encapsulated strain of Staphylococcus epidermidis isolated from a human clinical specimen was demonstrated by electron microscopy. The outermost layer of the cell wall of these organisms probably consists mostly of polysaccharide.

Cited by 34 publications

References 8 publications

Adhesion of Staphylococcus epidermidis and Staphylococcus saprophyticus to a Hydrophobic Biomaterial

Adhesion of Staphylococcus epidermidis and Staphylococcus saprophyticus to a Hydrophobic Biomaterial

Cross protection between an encapsulated strain of Staphylococcus hyicus and encapsulated strains of Staphylococcus aureus

The slime of coagulase-negative staphylococci: Biochemistry and relation to adherence

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