Isolation of an enterovirus-like agent from the meconium of dead-in-shell chicken embryos

Spackman, D; Re, Gough; Ms, Collins; Lanning, D. G.

doi:10.1136/vr.114.9.216-a

Cited by 44 publications

(43 citation statements)

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“…The isolation of enteroviruses and enterovirus-like viruses from chickens has been reported previously (Butterfield et al, 1969;Yamaguchi et al, 1979;McNulty et al" , 1987Spackman et al, 1984;Decaesstecker et al, 1986;Takase et al, 1989;Shirai et al, 1992). Several of these viruses have been compared by cross-indirect immunofluorescence and cross-serum neutralization tests and 5 serogroups identified (Takase et al, 1989;McNulty et al, 1990;Shirai étal, 1991Shirai étal, , 1992.…”

Section: Discussionmentioning

confidence: 99%

“…These include avian nephritis virus (ANV) which was first isolated from the rectal contents of a clinically normal broiler chicken in Japan (Yamaguchi et al, 1979), two enterovirus-like viruses (ELV-1, ELV-2) isolated from the faeces of broiler chickens , a virus (BELV-1) isolated from broiler chickens with runting stunting syndrome in Belgium (Decaesstecker et al, 1986) and FP3 virus, isolated from the meconium of dead-in-shell chicken embryos in England (Spackman et al } 1984).…”

Section: Introductionmentioning

confidence: 99%

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Studies on a new enterovirus‐like virus isolated from chickens

et al. 1994

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SUMMARYDuring investigations into an outbreak of respiratory distress in broilers chicks, a small round virus was isolated following inoculation into chicken embryos. The isolate, designated 612, was identified as an enterovirus-like virus on the basis of its size and morphology, resistance to chloroform and to treatment at pH 3.0, and intracytoplasmic replication in cell culture. The virus produced a partial cytopathic effect following inoculation into chick embryo kidney cell cultures and viral antigens could be detected by immunostaining. The preferred culture method for 612 virus was by inoculation onto the CAM of chick embryos. Cross-immunofluorescence indicated that the virus is not antigenically related to five previously identified chicken enterovirus/enterovirus-like virus serogroups. Following experimental inoculation of 1-day-old male broilers a number of which had maternal antibody to 612, growth retardation ranging from 9.6 to 20.4% was detected. Serological studies demonstrated antibody to 612 virus was widespread in commercial chicken flocks in N. Ireland.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Studies on a new enterovirus‐like virus isolated from chickens

et al. 1994

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“…English enterovirus-like viruses. The FP-3 isolate was isolated from the meconium of dead-in-shell chick embryos at the Central Veterinary Laboratory, Weybridge, Surrey, England (Spackman et al, 1984). The 1821/9 isolate was also isolated at Weybridge from the gut contents of 1-week-old broilers.…”

Section: Enterovirus-like Virusmentioning

confidence: 99%

“…These include viruses isolated from the faeces of broiler chickens with runting-stunting syndrome in Northern Ireland (McFerran et al, 1983;McNulty et al 1984) and Belgium (Meulemansef al,986; Decaessteckere/a/., 986), a virus isolated in England from the meconium of dead-in-shell chicken embryos (Spackman et al, 1984) and a virus isolated from the faeces of a 27-day-old broiler in Northern Ireland . This paper describes the antigenic relationship of these viruses to AEV and ANV, their growth properties in a number of cell cultures and embryonated fowl eggs and some aspects of their pathogenicity for chickens.…”

Section: Introductionmentioning

confidence: 99%

Biological characterisation of avian enteroviruses and enterovirus‐like viruses

et al. 1990

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SUMMARYThe antigenic relationships and in vitro growth properties of 10 avian enteroviruses and enterovirus-like viruses were investigated. By cross-indirect immunofluorescence tests, six serogroups were identified. The lack of an antigenic relationship between viruses selected from three of these serogroups was confirmed by cross-neutralisation tests. Most of the viruses grew poorly or not at all in a range of primary avian cell cultures, but the majority grew following inoculation of chick embryos via the yolk sac.

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“…Thus, the 612 isolate of CAstV, described by McNeilly et al (1994), shares a close antigenic and genetic relationship with the first characterized P22-18.8.00 isolate, but differs antigenically and genetically from the 11672 and FP3 CAstV isolates (Todd et al, 2009a). The FP3 isolate, originally recognized as an enterovirus-like virus (ELV), which was isolated from the meconium of dead-in-shell chicks during an investigation of early broiler mortality in the UK (Spackman et al, 1984), is closely related antigenically and genetically to the 11672 isolate, which was isolated in 2005 in our laboratories from one-day-old chicks as part of an investigation into hatchability problems in the UK. A recent study into the capsid protein sequence diversity of 25 CAstVs sourced mainly from the UK, but also including CAstVs from the USA, Europe (isolate P22-18.8.00) and South Africa (isolate 612), showed that CAstVs could be assigned to one of two major capsid groups, designated A and B, which shared 38-40% amino acid identities (Smyth et al, 2012).…”

Section: Introductionmentioning

confidence: 99%

Development of an indirect enzyme-linked immunosorbent assay test for detecting antibodies to chicken astrovirus in chicken sera

et al. 2015

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The development of an indirect enzyme-linked immunosorbent assay (ELISA) for the serological diagnosis of Group B chicken astrovirus (CAstV) infections is described. The test was based on the use of an affinity-purified capsid antigen, specific to CAstV isolate 11672, produced as a glutathione-S-transferase N-terminal fusion protein by a recombinant baculovirus. Strongly positive ELISA signals were elicited against experimentally produced antisera raised to CAstVs from Group B (subgroups i and ii) but were negative for antisera raised to a Group A CAstV. Using a panel of 240 selected serum samples, 99% agreement was observed when the results obtained by ELISA were compared to those from an indirect immunofluorescence test for CAstV 11672. The ELISA test was applied to 68 serum sets comprising 1864 samples, which were obtained from parent and grandparent flocks originating mainly in the UK. Of the 52 sets containing ELISA-positive samples, 24 sets had >75% samples positive and nine sets had <25% samples positive and were regarded as having high and low seropositivities, respectively. Of the 1864 serum samples tested 1090 (58.5%) were ELISA positive and of these, 234 sera (21.5%) produced strongly positive signals, whereas moderately positive and weakly positive signals were produced by 562 (51.5%) and 294 (27%) sera. When used for flock screening purposes, this ELISA test can be used to (i) investigate the occurrence of first-time CAstV infections of parent flocks during lay and the possible adverse effects caused by vertically transmitted CAstV infections on broiler hatchability and performance and (ii) diagnose Group B CAstV infections within specific pathogen free flocks.

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Isolation of an enterovirus-like agent from the meconium of dead-in-shell chicken embryos

Cited by 44 publications

References 0 publications

Studies on a new enterovirus‐like virus isolated from chickens

Studies on a new enterovirus‐like virus isolated from chickens

Biological characterisation of avian enteroviruses and enterovirus‐like viruses

Development of an indirect enzyme-linked immunosorbent assay test for detecting antibodies to chicken astrovirus in chicken sera

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