Isolation of an Inhibitor of β-Glucuronidase from Human Saliva

Sakamoto, Wataru; Nishikaze, O; Sakakibara, Eiichi

doi:10.1093/oxfordjournals.jbchem.a130439

Cited by 4 publications

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“…Fortunately, the recent work of Sakamoto et al. (15)(16)(17)(18) has provided a solution to this problem. In retrospect, it is now apparent that much of the published literature on glucuronidase excretion is of uncertain validity.…”

Section: Introductionmentioning

confidence: 99%

Coordinacy of lysosomal enzyme excretion in human urine.

Paigen¹,

Peterson²

1978

J. Clin. Invest.

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A B S T R A C T Assay conditions have been developed for the determination of urinary 8-glucuronidase, ,8-galactosidase, a-galactosidase, and, -hexosaminidase using fluorometric substrates. The assay conditions for ,8-glucuronidase overcome interference by both low and high molecular weight inhibitors, a problem that has confused earlier studies of enzyme excretion.The fouir lysosomal enzymes are excreted coordinately; although their absolute levels (in units per milligram of creatinine) vary during the day and from one day to the next, the ratio of one enzyme to another remains relatively constant. The lack of correlationi between plasma aind urine enzyme levels, together with the high molecular weights of these enzymes, suggests that the urinary enzymes are not derived by glomerular filtration. The lack of coordinacy with lactate dehydrogenase suggests they are not derived from exfoliated cells. By analogy with experimental animals, they' may be derived from lysosomes extruded into the lumnen of the proxinmal tubule by epithelial cells.There is considerable variation among a poptulation of 125 healthy adult subjects for total enzyme exeretionl. Both total enzyme excretion and coordinacy ratios are log-normially' distributed, suggesting that they are the resuiltants of many factors, each of which has a relative, or proportional, effect on enzyme excretion. About one-half the population variation resides in a process common to the exeretion of all four enzymes (possibly the lsosomiie extruisioni pathway'), and about one-half r-esides in factors affectiing each enzviye independently.

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Section: Introductionmentioning

confidence: 99%