Isolation of Antibiotic Resistant Bacteria from Rivers in Kelantan, Malaysia

Hassan, Nurul Liyana; Yern, Kam Kar; Zain, Nor Azimah Mohd

doi:10.38001/ijlsb.712542

Cited by 7 publications

(4 citation statements)

References 10 publications

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“…For years, a culture-dependent method would be relied upon to identify a microbial community in various settings, including the indoor air [11][12][13]. The collection of indoor air samples is a crucial step as it requires drawing a sample that represents the exposure of bacterial community which is present in the given location.…”

Section: Discussionmentioning

confidence: 99%

“…Studies on indoor air microbiomes may help to understand the potential causative agents that contribute to nosocomial infections. In the past, microbial communities in indoor air and water sources were often identi ed using a culture-dependent method [11][12][13], which involved collecting samples and culturing bacteria on agar plates using NMAM 0800 [14][15][16]. This method had limitations, including the inability to culture certain microbes and being timeconsuming.…”

Section: Introductionmentioning

confidence: 99%

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May the Pathogenic Microbes Not Be with You: Core Microbiome Profiling in Hospital Airspace

Hussin,

Tay,

Zainulabid

et al. 2024

Preprint

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The hospital indoor air microbiome, a diverse range of microorganisms, gains prominence amid the COVID-19 pandemic. Elevated awareness underscores implications for patient and staff well-being. Concerns about risks to indoor air quality persist due to prolonged indoor exposure, necessitating further research on specific threats within the hospital environment. In this study, an independent culture-based approach was used to analyze the baseline core microbiome present in hospital environments, utilizing amplicon sequencing on the next-generation sequencing technology to target the V3 region of the 16S rRNA gene. Firmicutes, Proteobacteria, and Actinobacteria were the main bacterial phyla that were most isolated from the wards and clinics with different orders of abundance; Firmicutes being associated more in clinics and Actinobacteriota in wards. The bacteria Niallia taxi, Methyloversatilis universalis, unclassified Rummeliibacillus, unclassified Clostridium, and unclassified Sphingomonadaceae dominated the clinic area while ward areas reported Pseudonocardia bannensis, Rubrobacter A aplysinae, unclassified Brachybacterium, unclassified Bradyrhizobium, and unclassified Mycobacterium to be the top five features. While the alpha-diversity index showed no significant differences, the beta-diversity analysis showed a significant differences between clinic and ward areas (p < 0.05). Certain bacterial species associated with opportunistic pathogens as well as normal skin flora such as Methylobacterium spp., Cutibacterium spp., unclassified Sphingomonadaceae, and Anoxybacillus B spp., were also identified across all samples. The methods described in this research aim to establish a rapid and sensitive screening process that could be valuable for disease surveillance within the healthcare setting, shedding light on the potential impacts of the hospital microbiome on human illness.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

May the Pathogenic Microbes Not Be with You: Core Microbiome Profiling in Hospital Airspace

Hussin,

Tay,

Zainulabid

et al. 2024

Preprint

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“…In Malaysia, the presence of Flavobacterium sp. is widely spread in freshwater habitats to the extent that some may have developed antibiotic resistance (Hassan et al, 2020). A study among the ornamental fish industry in Malaysia has also found the presence of this genus infecting fishes sold in local retail pet shops (Anjur et al, 2021).…”

Section: Importance and Application Of Bacterial Species Found In Put...mentioning

confidence: 99%

Untitled

2023

JST

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This study determines the associations between the bacterial communities and water physicochemical parameters in Putrajaya Lake and Putrajaya Wetlands Park, Malaysia. Bacterial communities were assessed by metagenomics of the 16S rRNA gene from lake water input, central wetlands, and primary lake area. Water samples (n=18) were collected during two different periods: post-high rainfall events (samples collected in May) and dry periods (July). The data revealed that bacterial communities of the three sites were taxonomically distinct and associated with different environmental parameters. However, no significant differences were found between the wet and dry periods. Alpha diversity analyses revealed the highest index in May 2018 in the constructed wetlands (H'= 5.397) than those from water input or primary lake (p<0.05). Overall, 49 phyla, 147 classes, 284 orders, 471 families, 778 genera and 62 species of bacteria were identified.

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“…Because of their better biodegradability than synthetic materials, plants can also reduce treatment costs or minimize the possibility of bacteria to develop drug resistance, in addition to their alternative uses to antibiotics (Reverter et al 2014;Salikan et al 2020;Hassan et al 2020). Pitahaya is a type of climbing cactus belonging to the genus Hylocereus sp.…”

Section: Introductionmentioning

confidence: 99%

Biological Activity of Red Pitahaya Extracts on Lactococcus garvieae and Vibrio alginolyticus

ÇELİK,

AŞAN ÖZÜSAĞLAM

2023

Manas Journal of Agriculture Veterinary and Life Sciences

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The enhancement population of the world every day brings with it the demand for protein needs. In our study, it was aimed to identify the utilization potential of red pitahaya fruit obtained from Turkey as a natural feed additive. The disc diffusion assay, micro-dilution method for determination of minimum inhibitory (MIC) and minimum bactericidal (MBC) concentration values of the extracts were used to identify the biological activity of the extracts from red pitahaya. The red pitahaya pulp methanol extract against L. garvieae and V. alginolyticus showed 10.61 mm and 7.65 mm of inhibition zone diameters. The methanol extract of red pitahaya peel has 10.18 mm and 11.25 mm inhibition zone diameters on L. garvieae and V. alginolyticus. MIC values were determined as 20 µg/µl and 40 µg/µl for pulp methanol extract and as 80 µg/µl and 40 µg/µl for peel methanol extract against L. garvieae and V. alginolyticus. MBC values of pulp and peel methanol extracts against fish pathogens were determined as 80 µg/µl. As a result, the red pitahaya extracts may have usage potential as natural antimicrobial agents or feed additive in aquaculture.

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Isolation of Antibiotic Resistant Bacteria from Rivers in Kelantan, Malaysia

Cited by 7 publications

References 10 publications

May the Pathogenic Microbes Not Be with You: Core Microbiome Profiling in Hospital Airspace

May the Pathogenic Microbes Not Be with You: Core Microbiome Profiling in Hospital Airspace

Untitled

Biological Activity of Red Pitahaya Extracts on Lactococcus garvieae and Vibrio alginolyticus

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