Isolation of anticancer and antimicrobial metabolites from Epicoccum nigrum; endophyte of Ferula sumbul

Perveen, Irum; Raza, Muhammad Asam; Iqbal, Tahir; Sehar, Shama; Ahmed, Safia

doi:10.1016/j.micpath.2017.06.033

Cited by 46 publications

(23 citation statements)

References 49 publications

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“…Previous studies showed that the fungus Epicoccum produces active substances such as flavipin, epirodins, epicorazines, and prodiginine to inhibit the growth of plant-pathogenic fungi [ 39 , 40 , 41 ]. Epicoccum sp.…”

Section: Discussionmentioning

confidence: 99%

The MAPKKK CgMck1 Is Required for Cell Wall Integrity, Appressorium Development, and Pathogenicity in Colletotrichum gloeosporioides

Fang

Xia

Wang

et al. 2018

Genes

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Mitogen-activated protein kinase (MAPK) signaling pathway plays key roles in sensing extracellular signals and transmitting them from the cell membrane to the nucleus in response to various environmental stimuli. A MAPKKK protein CgMck1 in Colletotrichum gloeosporioides was characterized. Phenotypic analyses of the ∆Cgmck1 mutant showed that the CgMck1 was required for vegetative growth, fruiting body development, and sporulation. Additionally, the CgMCK1 deletion mutant showed significant defects in cell wall integrity, and responses to osmotic stresses. The mutant abolished the ability to develop appressorium, and lost pathogenicity to host plants. The ∆Cgmck1 mutant also exhibited a higher sensitivity to antifungal bacterium agent Bacillus velezensis. The deletion mutants of downstream MAPK cascades components CgMkk1 and CgMps1 showed similar defects to the ∆Cgmck1 mutant. In conclusion, CgMck1 is involved in the regulation of vegetative growth, asexual development, cell wall integrity, stresses resistance, and infection morphogenesis in C. gloeosporioides.

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Section: Discussionmentioning

confidence: 99%

The MAPKKK CgMck1 Is Required for Cell Wall Integrity, Appressorium Development, and Pathogenicity in Colletotrichum gloeosporioides

Fang

Xia

Wang

et al. 2018

Genes

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“…Talontis et al (2013) characterized three poly-oxygenated polyketides named epicolactone, epiccocclides A, and epiccocclides B which showed an antimicrobial effect against several pathogenic bacteria such as Bacillus subtilis , Staphylococcus aureus, and Escherichia coli [23]. Perveen et al (2017) isolated 2-methyl-3-nonyl prodiginine, bis (2-ethylhexyl) phthalate, and preaustinoid A, and found that these compounds could be used as antimicrobial agents [24]. Flavipin, a yellow crystalline powder produced by E. nigrum and other fungi has been claimed to be involved in the inhibitory effect due to its antifungal activity [25].…”

Section: Discussionmentioning

confidence: 99%

Fungal Endophytes Control Fusarium graminearum and Reduce Trichothecenes and Zearalenone in Maize

Abdallah

Boevre

Landschoot

et al. 2018

Toxins

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Fusarium graminearum can cause Giberella Ear Rot (GER) and seedling blight in maize, resulting in major yield losses. Besides GER, the infected grains are consequently contaminated with multiple mycotoxins of F. graminearum. Zearalenone and trichothecenes, such as deoxynivalenol and its acetylated forms, are among the major mycotoxins associated with F. graminearum infection in maize. In the current work, we explored the effect of the endophytic fungal genera of Epicoccum and Sordaria, to control F. graminearum infection in comparative trials with Piriformospora spp., an elusive endophytic genus. Furthermore, we investigated the effect of these endophytes on zearalenone, deoxynivalenol, and 15-acetyldeoxynivalenol levels using in vitro and in planta assays. As plants are endowed with several detoxification mechanisms comprising e.g., glucosylation of trichothecenes, the effect of the isolated fungal endophytes on the deoxynivalenol-3-glucoside level was also assessed. In general, results showed a considerable variability in the antifungal activity, both among species and among isolates within one species. Additionally, the effect on mycotoxin levels was variable, and not necessarily related to the antifungal activity except for zearalenone levels which were consistently reduced by the endophytes. These results highlight the great potential of certain endophytic fungal strains as new biocontrol agents in agricultural science.

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“…E. nigrum has been used as a tool of biological control for plant pathogens and produces a variety of bioactive compounds such as flavipin (Bamford et al 1961), epicorazins A-B (Baute et al 1978), epirodin (Ikawa et al 1978), orevactaene (Shu et al 1997), epicocconone (Bell et al 2003), epicoccins A-D (Zhang et al 2007) and epicolactone (Silva et al 2012). Recently, Perveen et al (2017) extracted three new agents (2-methyl-3-nonyl prodiginine, Bis (2-ethylhexyl) phthalate and a meroterpenoid, Preaustinoid A), which displayed anticancer and antimicrobial activities, from E. nigrum. Future studies will be directed toward structure determination of effective compounds from Colletotrichum sp.…”

Section: Analysing Extract Bioactivitiesmentioning

confidence: 99%

Antimicrobial Activity of Fungal Endophytes from Vaccinium dunalianum var. urophyllum

Tong¹,

Xiao²,

Cheng³

2018

JSM

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Fungi associated with Vaccinium species play important roles in plant growth and disease control, especially in the final blueberry production. Vaccinium dunalianum var. urophyllum (Ericaceae) is a well-known medicinal plant in Southern China used to treat inflammation and microbial infections. The endophytic fungi from these plants are therefore anticipated as potential new sources of antimicrobials. In this report, the inhibitory effects of endophytes against clinical bacteria and yeast were comprehensively screened and 11 isolates indicated high bioactivity by the agar diffusion method. The corresponding crude extracts of these fungi under submerged fermentation also demonstrated distinct differences and n-butyl alcohol displayed the lowest extraction efficiency among the extracts. The ethyl acetate and dichloromethane extracts of filtrates from the Colletotrichum sp. VD001, Epicoccum nigrum VD021 and E. nigrum VD022 strains displayed good properties against pathogenic microorganisms according to disc diffusion assays and minimal inhibitory concentration (MIC). This study is the first indicating that cultivable endophytic fungi associated with blueberry plants produce potential compounds against clinical pathogens. ABSTRAKKulat yang dikaitkan dengan spesies Vaccinium memainkan peranan penting dalam pertumbuhan tumbuhan dan kawalan penyakit, terutamanya dalam pengeluaran beri biru akhir. Vaccinium dunalianum var. urophyllum (Ericaceae) ialah tumbuhan ubatan yang terkenal di Selatan China yang digunakan untuk merawat jangkitan keradangan dan mikrob. Oleh itu, kulat endofit daripada tumbuhan ini dijangka sebagai sumber baru antimikrob yang berpotensi. Dalam laporan ini, kesan perencatan endofit terhadap bakteria klinikal dan suatu ragi telah ditayangkan secara komprehensif dan 11 pencilan menunjukkan bioaktiviti tinggi melalui kaedah penyebaran agar. Ekstrak mentah yang sepadan dengan kulat ini di bawah penapaian terendam juga menunjukkan perbezaan yang ketara serta n-butil alkohol menunjukkan kecekapan pengekstrakan terendah dalam kalangan ekstrak. Ekstrak etil asetat dan diklorometana filtrat daripada Colletotrichum sp. VD001, Epicoccum nigrum VD021 dan E. nigrum VD022 menunjukkan sifat-sifat yang baik terhadap mikroorganisma patogen berdasarkan ujian penyebaran cakera dan kepekatan perencatan minimum (MIC). Kajian ini adalah kajian yang pertama menunjukkan bahawa kulat endofit yang boleh dikaitkan dengan tumbuhan beri biru menghasilkan sebatian yang berpotensi terhadap patogen klinikal.Kata kunci: Aktiviti antimikrob; kepekatan perencatan minimum; kulat endofit; spesies Vaccinium

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Isolation of anticancer and antimicrobial metabolites from Epicoccum nigrum; endophyte of Ferula sumbul

Cited by 46 publications

References 49 publications

The MAPKKK CgMck1 Is Required for Cell Wall Integrity, Appressorium Development, and Pathogenicity in Colletotrichum gloeosporioides

The MAPKKK CgMck1 Is Required for Cell Wall Integrity, Appressorium Development, and Pathogenicity in Colletotrichum gloeosporioides

Fungal Endophytes Control Fusarium graminearum and Reduce Trichothecenes and Zearalenone in Maize

Antimicrobial Activity of Fungal Endophytes from Vaccinium dunalianum var. urophyllum

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