Isolation of Antigen-Specific Unconventional Bovine Ultra-Long CDR3H Antibodies Using Cattle Immunization in Combination with Yeast Surface Display

Arras, Paul; Zimmermann, Jasmin; Lipinski, Britta; Yanakieva, Desislava; Klewinghaus, Daniel; Krah, Simon; Kolmar, Harald; Pekar, Lukas; Zielonka, Stefan

doi:10.1007/978-1-0716-3279-6_8

Methods in Molecular Biology

2023

DOI: 10.1007/978-1-0716-3279-6_8

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Isolation of Antigen-Specific Unconventional Bovine Ultra-Long CDR3H Antibodies Using Cattle Immunization in Combination with Yeast Surface Display

Paul Arras

Jasmin Zimmermann

Britta Lipinski

et al.

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2024

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Bovine ultralong CDR-H3 derived knob paratopes elicit potent TNF-α neutralization and enable the generation of novel adalimumab-based antibody architectures with augmented features

Arras,

Zimmermann,

Lipinski

et al. 2024

Biological Chemistry

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In this work we have generated cattle-derived chimeric ultralong CDR-H3 antibodies targeting tumor necrosis factor α (TNF-α) via immunization and yeast surface display. We identified one particular ultralong CDR-H3 paratope that potently neutralized TNF-α. Interestingly, grafting of the knob architecture onto a peripheral loop of the CH3 domain of the Fc part of an IgG1 resulted in the generation of a TNF-α neutralizing Fc (Fcknob) that did not show any potency loss compared with the parental chimeric IgG format. Eventually, grafting this knob onto the CH3 region of adalimumab enabled the engineering of a novel TNF-α targeting antibody architecture displaying augmented TNF-α inhibition.

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Bovine ultralong CDR-H3 derived knob paratopes elicit potent TNF-α neutralization and enable the generation of novel adalimumab-based antibody architectures with augmented features

Arras,

Zimmermann,

Lipinski

et al. 2024

Biological Chemistry

View full text Add to dashboard Cite

show abstract

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Isolation of Antigen-Specific Unconventional Bovine Ultra-Long CDR3H Antibodies Using Cattle Immunization in Combination with Yeast Surface Display

Cited by 1 publication

References 20 publications

Bovine ultralong CDR-H3 derived knob paratopes elicit potent TNF-α neutralization and enable the generation of novel adalimumab-based antibody architectures with augmented features

Bovine ultralong CDR-H3 derived knob paratopes elicit potent TNF-α neutralization and enable the generation of novel adalimumab-based antibody architectures with augmented features

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