Isolation of apoproteins from canine surface active material

King, RJ; Klass, DJ; Gikas, E; Clements, J. A.

doi:10.1152/ajplegacy.1973.224.4.788

Cited by 239 publications

(86 citation statements)

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“…Almost all of the protein in bronchoalveolar lavage is found associated with surfactant lipids. SP-A was the first of the surfactant proteins that was purified [171] and analyzed for its primary structure [343]. Butanol extraction is a widely used method to purify SP-A, but recent work from our laboratory suggests that some of the functional characteristics of SP-A are lost during this extraction procedure [317].…”

Section: Structure Of Sp-amentioning

confidence: 99%

“…In 1975 Hallman and co-workers discovered the importance of phosphatidylglycerol (PG) in contributing to surfactant spreading and the decreased levels of this phospholipid in children suffering from respiratory distress syndrome (RDS) [112]. The demonstration of a protein in surfactant was important for the recognition that proteins could be important constituents of surfactant [171]. A landmark was the first successful treatment of neonatal RDS with surfactant replacement therapy [87].…”

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confidence: 99%

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The Pulmonary Surfactant System: Biochemical and Clinical Aspects

Creuwels

Golde

Haagsman

1997

Lung

283

188

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Abstract. This article starts with a brief account of the history of research on pulmonary surfactant. We will then discuss the morphological aspects and composition of the pulmonary surfactant system. We describe the hydrophilic surfactant proteins A and D and the hydrophobic surfactant proteins B and C, with focus on the crucial roles of these proteins in the dynamics, metabolism, and functions of pulmonary surfactant. Next we discuss the major disorders of the surfactant system. The final part of the review will be focused on the potentials and complications of surfactant therapy in the treatment of some of these disorders. It is our belief that increased knowledge of the surfactant system and its functions will lead to a more optimal composition of the exogenous surfactants and, perhaps, widen their applicability to treatment of surfactant disorders other than neonatal respiratory distress syndrome.Key words: Surfactant protein-Pulmonary surfactant-Respiratory distress syndrome. HistoryResearch on surfactant goes back to 1929 when von Neergaard published the first paper about the difference in pressure needed to inflate lungs with air or with liquid [333]. He found that the pressure necessary for filling the lungs with air was higher than when the lungs were filled with liquid. To explain this result he stated that the alveoli were stabilized by lowering the naturally high surface tension of the air/water interface. In 1946 Thannhauser and co-workers reported that lung tissue has a remarkably high content of the lipid dipalmityl lecithin (current name, dipalmitoylphosphatidylcholine)Offprint requests to: Henk P. Haagsman.

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Section: Structure Of Sp-amentioning

confidence: 99%

mentioning

confidence: 99%

The Pulmonary Surfactant System: Biochemical and Clinical Aspects

Creuwels

Golde

Haagsman

1997

Lung

283

188

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show abstract

“…Most of lipids in the precipitate fraction of the pulmonary washings were extracted with ether and ethanol according to the method of King et al (1973), and the proteins obtained after lipid removal were solubilized in 5 mM Tris-HC1 buffer (pH 7.5) containing 5 M urea and 0.02% 2-mercaptoethanol and applied to a column (2.5>< 80 cm) of Sephadex G 200 equilibrated with the same buffer. The elution of peptides was done with the equilibrating buffer.…”

Section: Protein Analysismentioning

confidence: 99%

“…The protein of the precipitate fraction exists in small amounts, and mainly comprises two glycoproteins, which are 62,000 and 36,000 molecular weight proteins, and which appear to be the same proteins (Bhattacharyya et al 1975) as known to be contained in the intra-and extracellular surfactant fractions of normal lung (King et al 1973;Sawada and Kashiwamata 1977). Bhattacharyya et al (1975) reported that glycoproteins isolated from the pulmonary washing of rabbits were identical in moleculr weights, and amino acid and carbohydrate compositions with those found in pulmonary washings of patients with alveolar proteinosis.…”

Section: Protein Analysismentioning

confidence: 99%

Biochemical characterization of pulmonary washings of patients with alveolar proteinosis, interstitial pneumonitis and alveolar cell carcinoma.

Onodera

Nakamura

Sato

et al. 1983

Tohoku J. Exp. Med.

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“…Mam~~ian pulmon~ry surfactant is composed primarily of phospholIpIds~nd ass?cIat~d proteins, Two major protein groups h~ve been prevIOusly IdentIfied: a surfactant-associated glycoprotem of 3~-40,000 daltons first described by King and Clements (1), hereI~called .SAP-35 and smaller molecular weight proteins det~cted m a v~net~of mammalian surfactants (1)(2)(3)(4)(5)(6)(7)(8)(9)(10)(11)(12). ClarificatIon of the IdentIty and nature of the various surfactantassociated proteins has been incomplete.…”

mentioning

confidence: 99%