Isolation of Ascomycetous Fungi, Neosartorya spp. and Screening for Its Antibacterial Metabolites

Abstract

 Abstract-The aim of the present study was to identify ascomycetous fungi (Neosartorya spp.) and examined the antibacterial activities of crude mycelium extracted by ethyl acetate from these fungal genera. Among 10 ascomycetous fungal isolates, Neosartorya sp., were screened from soil and showed a broader antimicrobial spectrum than the other. Especially, fungal strain KKU-1NK1 showed strongly antibacterial activities. This fungal strain was belonging to Neosartorya spinosa which was identified using 5.8S rRNA gene sequencing analysis. The results revealed that the growth of clinical isolates of gram-positive pathogenic bacteria (Methicillin-resistant Staphylococcus aureus DMST 20654, Staphylococcus aureus ATCC 25923, Staphylococcus saprophyticus ATCC 15305, Streptococcus pneumonia DMST 15319 and Bacillus subtilis ATCC 6633) was well inhibited by crude mycelial extracted from N. spinosa KKU-1NK1 at concentrations values of 10 mg/ml. Moreover, the effects of submerge culture media variation on mycelia growth which affected to total production of bioactive compounds and antibacterial activity by N. spinosa KKU-1NK1 was observed.

Isolation, Characterization and Screening for Anticancer and Antimicrobial Properties of the Crude Extract from Genus Neosartorya

“…The dendrogram of calmodulin gene was demonstated representative of these fungi (Figure 3). Our result was supported with findings of Sanmanoch et al [4] who reported the nucleotide sequences from the ITS region is useful and suitable for Neosartorya identification. However, identification of Neosartorya should be using both molecular and morphological techniques [12].…”

“…Crude extracts from Neosartorya showed well inhibited the growth of gram positive bacteria (B. subtilis, S. aureus and M. luteus) ( Table 2). Our result was supported with findings of Sanmanoch et al [4] who reported to the antibacterial activities of crude ethyl acetate extract from N. spinosa KKU1NK1 most strongly inhibited the growth of gram positive pathogenic bacteria (S. aureus, S. saprophyticus, S. pneumonia and B. subtilis).…”

“…(Table 1). Our findings are consistent with the some species of the genus Neosartorya such as N. pseudofischeri, N. tatenoi and N. spinosa have been reported for compounds which potent cytotoxicity against the mammalians and insect cell lines [1][2][3][4]13].…”

“…Including, a new meroterpenoid: tatenoic acid was isolated from the fungus N. tatenoi exhibited cytotoxicity against human small cell lung cancer (NCI-H187) and human epidermoid carcinoma in the mouth (KB) [3]. Additionally, the crude mycelial extracted from N. spinosa were reported as antibacterial [4]. The screening for bioactive compounds from the Neosartorya is challenging.…”

Filamentous Fungi PKF121 Isolated from Dry Dipterocarp Forest Soil in Northeast Thailand Produces Antimicrobial Agents Active Aginst Methicillin-Resistant Staphylococcus aureus

“…[28][29][30][31]. However, P. citrinum with antimicrobial and antiyeast activities was reported from Surat Thani province in southern part of Thailand [32].…”