Isolation of Ascomycota fungi with capability to transform PAHs: Insights into the biodegradation mechanisms of Penicillium oxalicum

Aranda, Elisabet; Godoy, Patricia; Reina, Rocío; Badia-Fabregat, Marina; Rosell, Mònica; Marco‐Urrea, Ernest; García-Romera, Inmaculada

doi:10.1016/j.ibiod.2017.05.015

Cited by 73 publications

(45 citation statements)

References 42 publications

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“…Aranda et al . (2017) mentioned that fungi belonging to this division resist stress conditions compared to other organisms. Because of this, they can grow in contaminated environments.…”

Section: Resultsmentioning

confidence: 99%

Analysis of phenanthrene degradation by Ascomycota fungi isolated from contaminated soil from Reynosa, Mexico

Cruz-Izquierdo

Paz-González

Reyes-Espinosa

et al. 2021

Lett Appl Microbiol

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Polycyclic aromatic hydrocarbons (PAHs) are organic compounds generated mainly by anthropogenic sources. They are considered toxic to mammals, since they have carcinogenic, mutagenic and genotoxic properties, among others. Although mycoremediation is an efficient, economical and eco‐friendly technique for degrading PAHs, the fungal degradation potential of the phylum Ascomycota has not been widely studied. In this work, we evaluated different fungal strains from the polluted soil of ‘La Escondida’ lagoon in Reynosa, Mexico to know their potential to degrade phenanthrene (PHE). Forty‐three soil isolates with the capacity to grow in the presence of PHE (0·1% w/v) were obtained. The fungi Aspergillus oryzae MF13 and Aspergillus flavipes QCS12 had the best potential to degrade PHE. Both fungi germinated and grew at PHE concentrations of up to 5000 mg l−1 and degraded 235 mg l−1 of PHE in 28 days, with and without an additional carbon source. These characteristics indicate that A. oryzae MF13 and A. flavipes QCS12 could be promising organisms for the remediation of sites contaminated with PAHs and detoxification of recalcitrant xenobiotics.

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“…Aranda et al . (2017) mentioned that fungi belonging to this division resist stress conditions compared to other organisms. Because of this, they can grow in contaminated environments.…”

Section: Resultsmentioning

confidence: 99%

Analysis of phenanthrene degradation by Ascomycota fungi isolated from contaminated soil from Reynosa, Mexico

Cruz-Izquierdo

Paz-González

Reyes-Espinosa

et al. 2021

Lett Appl Microbiol

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“…Wu et al, (2020a) and Wu et al, (2020b) reported the presence of Ochroconis species in two different petroleumcontaminated soils in China at relative abundances of 1-5%. Aranda et al, (2015) reported the ability of Pseudallescheria isolates to degrade PAHs, and Covino et al, (2015) found that a species of Pseudallescheria was highly efficient (71% removal rates) at degrading aliphatic hydrocarbons from clayey soils. The presence of these groups of fungi in the Ecopile soil suggests that they have a possible role in hydrocarbon degradation.…”

Section: Discussionmentioning

confidence: 99%

Soil Microbiome Structure and Function in Ecopiles Used to Remediate Petroleum-Contaminated Soil

Wang

Garrido‐Sanz

Sansegundo‐Lobato

et al. 2021

Front. Environ. Sci.

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The soil microbiome consists of a vast variety of microorganisms which contribute to essential ecosystem services including nutrient recycling, protecting soil structure, and pathogen suppression. Recalcitrant organic compounds present in soils contaminated with fuel oil can lead to a decrease in functional redundancy within soil microbiomes. Ecopiling is a passive bioremediation technique involving biostimulation of indigenous hydrocarbon degraders, bioaugmentation through inoculation with known petroleum-degrading consortia, and phytoremediation. The current study investigates the assemblage of soil microbial communities and pollutant-degrading potential in soil undergoing the Ecopiling process, through the amplicon marker gene and metagenomics analysis of the contaminated soil. The analysis of key community members including bacteria, fungi, and nematodes revealed a surprisingly diverse microbial community composition within the contaminated soil. The soil bacterial community was found to be dominated by Alphaproteobacteria (60–70%) with the most abundant genera such as Lysobacter, Dietzia, Pseudomonas, and Extensimonas. The fungal community consisted mainly of Ascomycota (50–70% relative abundance). Soil sequencing data allowed the identification of key enzymes involved in the biodegradation of hydrocarbons, providing a novel window into the function of individual bacterial groups in the Ecopile. Although the genus Lysobacter was identified as the most abundant bacterial genus (11–46%) in all of the contaminated soil samples, the metagenomic data were unable to confirm a role for this group in petrochemical degradation. Conversely, genera with relatively low abundance such as Dietzia (0.4–9.0%), Pusillimonas (0.7–2.3%), and Bradyrhizobium (0.8–1.8%) did possess genes involved in aliphatic or aromatic compound degradation.

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“…Most of the strains isolated after the enrichment steps belonged to the phylum Ascomycota, although few Basidiomycota were isolated. The dominance of Ascomycota in polluted soil has been extensively acknowledged: Zhang et al (2018) reported that Ascomycota represented up to 73–96% of the total 18S sequences of a coking area soil; eight of the 10 strains isolated from a PAHs contaminated pond were Ascomycetes and were also able to remove anthracene ( Aranda et al, 2017 ). Finally, an oilfield with a history of 50 years pollution influenced mainly the bacterial community, whereas fungi, mostly related to this phylum, were abundant, demonstrating to be less sensitive to soil PAHs ( Zhou et al, 2017 ).…”

Section: Discussionmentioning

confidence: 99%

Molecular and Microbiological Insights on the Enrichment Procedures for the Isolation of Petroleum Degrading Bacteria and Fungi

et al. 2018

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Autochthonous bioaugmentation, by exploiting the indigenous microorganisms of the contaminated environment to be treated, can represent a successful bioremediation strategy. In this perspective, we have assessed by molecular methods the evolution of bacterial and fungal communities during the selective enrichment on different pollutants of a soil strongly polluted by mixtures of aliphatic and polycyclic hydrocarbons. Three consecutive enrichments were carried out on soil samples from different soil depths (0–1, 1–2, 2–3 m), and analyzed at each step by means of high-throughput sequencing of bacterial and fungal amplicons biomarkers. At the end of the enrichments, bacterial and fungal contaminants degrading strains were isolated and identified in order to (i) compare the composition of enriched communities by culture-dependent and culture-independent molecular methods and to (ii) obtain a collection of hydrocarbon degrading microorganisms potentially exploitable for soil bioremediation. Molecular results highlighted that for both bacteria and fungi the pollutant had a partial shaping effect on the enriched communities, with paraffin creating distinct enriched bacterial community from oil, and polycyclic aromatic hydrocarbons generally overlapping; interestingly neither the soil depth or the enrichment step had significant effects on the composition of the final enriched communities. Molecular analyses well-agreed with culture-dependent analyses in terms of most abundant microbial genera. A total of 95 bacterial and 94 fungal strains were isolated after selective enrichment procedure on different pollutants. On the whole, isolated bacteria where manly ascribed to Pseudomonas genus followed by Sphingobacterium, Bacillus, Stenothrophomonas, Achromobacter, and Serratia. As for fungi, Fusarium was the most abundant genus followed by Trichoderma and Aspergillus. The species comprising more isolates, such as Pseudomonas putida, Achromobacter xylosoxidans and Ochromobactrum anthropi for bacteria, Fusarium oxysporum and Fusarium solani for fungi, were also the dominant OTUs assessed in Illumina.

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Isolation of Ascomycota fungi with capability to transform PAHs: Insights into the biodegradation mechanisms of Penicillium oxalicum

Cited by 73 publications

References 42 publications

Analysis of phenanthrene degradation by Ascomycota fungi isolated from contaminated soil from Reynosa, Mexico

Analysis of phenanthrene degradation by Ascomycota fungi isolated from contaminated soil from Reynosa, Mexico

Soil Microbiome Structure and Function in Ecopiles Used to Remediate Petroleum-Contaminated Soil

Molecular and Microbiological Insights on the Enrichment Procedures for the Isolation of Petroleum Degrading Bacteria and Fungi

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