Isolation of Bacillus amyloliquefaciens Strains with Antifungal Activities from Meju

Lee, Hwang A; Kim, Jeong Hwan

doi:10.3746/pnf.2012.17.1.064

Cited by 20 publications

(10 citation statements)

References 18 publications

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“…The difference in inhibition efficiency also indicated that media composition has an effect on anti- P. palmivora metabolite production. Although generally, TSB is the richest nutrient composition medium when compared with NB and LB media, and can well support the growth of several bacteria, TSB and NB may interfere with antifungal production as previously observed [ 31 , 32 ]. The original conditions, pH 7 and 37 °C, seemed to be optimal and demonstrated the highest percentage of inhibition when compared with other tested conditions.…”

Section: Discussionmentioning

confidence: 74%

Biocontrol of Orchid-pathogenic Mold,Phytophthora palmivora, by Antifungal Proteins fromPseudomonas aeruginosaRS1

Sowanpreecha

Rerngsamran

2018

Mycobiology

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Black rot disease in orchids is caused by the water mold Phytophthora palmivora. To gain better biocontrol performance, several factors affecting growth and antifungal substance production by Pseudomonas aeruginosa RS1 were verified. These factors include type and pH of media, temperature, and time for antifungal production. The results showed that the best conditions for P. aeruginosa RS1 to produce the active compounds was cultivating the bacteria in Luria-Bertani medium at pH 7.0 for 21 h at 37 °C. The culture filtrate was subjected to stepwise ammonium sulfate precipitation. The precipitated proteins from the 40% to 80% fraction showed antifungal activity and were further purified by column chromatography. The eluted proteins from fractions 9–10 and 33–34 had the highest antifungal activity at about 75% and 82% inhibition, respectively. SDS-PAGE revealed that the 9–10 fraction contained mixed proteins with molecular weights of 54 kDa, 32 kDa, and 20 kDa, while the 33–34 fraction contained mixed proteins with molecular weights of 40 kDa, 32 kDa, and 29 kDa. Each band of the proteins was analyzed by LC/MS to identify the protein. The result from Spectrum Modeler indicated that these proteins were closed similarly to three groups of the following proteins; catalase, chitin binding protein, and protease. Morphological study under scanning electron microscopy demonstrated that the partially purified proteins from P. aeruginosa RS1 caused abnormal growth and hypha elongation in P. palmivora. The bacteria and/or these proteins may be useful for controlling black rot disease caused by P. palmivora in orchid orchards.

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Section: Discussionmentioning

confidence: 74%

Biocontrol of Orchid-pathogenic Mold,Phytophthora palmivora, by Antifungal Proteins fromPseudomonas aeruginosaRS1

Sowanpreecha

Rerngsamran

2018

Mycobiology

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“…Activity was expressed as an arbitrary unit (AU) per 1 mL and 1 AU was defined as the highest dilution showing a clear zone of inhibition (16).…”

Section: Methodsmentioning

confidence: 99%

“…Indicators are listed in Table 1. When fungi were used as indicators, potato dextrose agar (PDA) top agar (0.7%, w/v) containing 1x10 5 fungal spores was overlaid on an LB plate (16). Holes were made in plates using a capillary glass tube and 100 µL of FS was placed into each hole.…”

Section: Methodsmentioning

confidence: 99%

Antimicrobial activity of Bacillus amyloliquefaciens EMD17 isolated from Cheonggukjang and potential use as a starter for fermented soy foods

Lee

Shim

Zhuang

et al. 2016

Food Sci Biotechnol

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A strain with antimicrobial activity was isolated from and identified as amyloliquefaciens. Filtered culture supernatants of EMD17 strongly inhibited growth of ATCC14579, ATCC19111, and an ochratoxin (OTA) producing spp. The antimicrobial activity was not decreased by heat, pH, and proteases treatment, indicating a non-proteinous nature of the antimicrobialsubstance. A EMD17 culture added to a ATCC14579 culture killed cells completely in 6 h, showing the bacteriocidal effect. The gene encoding surfactin synthetase A was detected using PCR, indicating that surfactin might be the responsible agent. Genes encoding fengycin and iturin were also detected. MS indicated production of lipopeptides, including surfactin. and were prepared using EMD17 as a starter and growth of spiked cells was completely inhibited.

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“…For sequencing gyrB , primers UP-1 ( ) and UP-2r ( ) were used for gyrB gene amplification, and UP-1S ( ) and UP-2Sr ( ) were used for sequencing [ 70 ]. For sequencing recA , primers rec A-F ( ) and rec A-R ( ) were used for both gene amplification and sequencing [ 71 ]. Each reaction for PCR has a total volume of 25 μl containing 2 μl of ~100 ng/μl of genomic DNA as a template, 2.5 μl of 10X PCR buffer (Agilent Technologies, Inc., Santa Clara, CA), 0.5 μl of 10 mM dNTPs, 0.2 μl of 5 U/μl of Paq5000 DNA polymerase (Agilent Technologies, Inc., Santa Clara, CA), 1 μl of 10 μM of forward and reverse primers, and 17.8 μl of sterilized ddH 2 O.…”

Section: Methodsmentioning

confidence: 99%

Biological Control Activities of Rice-Associated Bacillus sp. Strains against Sheath Blight and Bacterial Panicle Blight of Rice

et al. 2016

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Potential biological control agents for two major rice diseases, sheath blight and bacterial panicle blight, were isolated from rice plants in this study. Rice-associated bacteria (RABs) isolated from rice plants grown in the field were tested for their antagonistic activities against the rice pathogens, Rhizoctonia solani and Burkholderia glumae, which cause sheath blight and bacterial panicle blight, respectively. Twenty-nine RABs were initially screened based on their antagonistic activities against both R. solani and B. glumae. In follow-up retests, 26 RABs of the 29 RABs were confirmed to have antimicrobial activities, but the rest three RABs did not reproduce any observable antagonistic activity against R. solani or B. glumae. According to16S rDNA sequence identity, 12 of the 26 antagonistic RABs were closest to Bacillus amyloliquefaciens, while seven RABs were to B. methylotrophicus and B, subtilis, respectively. The 16S rDNA sequences of the three non-antagonistic RABs were closest to Lysinibacillus sphaericus (RAB1 and RAB12) and Lysinibacillus macroides (RAB5). The five selected RABs showing highest antimicrobial activities (RAB6, RAB9, RAB16, RAB17S, and RAB18) were closest to B. amyloliquefaciens in DNA sequence of 16S rDNA and gyrB, but to B. subtilis in that of recA. These RABs were observed to inhibit the sclerotial germination of R. solani on potato dextrose agar and the lesion development on detached rice leaves by artificial inoculation of R. solani. These antagonistic RABs also significantly suppressed the disease development of sheath blight and bacterial panicle blight in a field condition, suggesting that they can be potential biological control agents for these rice diseases. However, these antagonistic RABs showed diminished disease suppression activities in the repeated field trial conducted in the following year probably due to their reduced antagonistic activities to the pathogens during the long-term storage in -70C, suggesting that development of proper storage methods to maintain antagonistic activity is as crucial as identification of new biological control agents.

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Isolation of Bacillus amyloliquefaciens Strains with Antifungal Activities from Meju

Cited by 20 publications

References 18 publications

Biocontrol of Orchid-pathogenic Mold,Phytophthora palmivora, by Antifungal Proteins fromPseudomonas aeruginosaRS1

Biocontrol of Orchid-pathogenic Mold,Phytophthora palmivora, by Antifungal Proteins fromPseudomonas aeruginosaRS1

Antimicrobial activity of Bacillus amyloliquefaciens EMD17 isolated from Cheonggukjang and potential use as a starter for fermented soy foods

Biological Control Activities of Rice-Associated Bacillus sp. Strains against Sheath Blight and Bacterial Panicle Blight of Rice

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