Isolation of bacterial culture capable of degrading triphenyltin pesticides

Visoottiviseth, Pornsawan; Kruawan, K.; Bhumiratana, Amaret; Wilairat, Prapin

doi:10.1002/aoc.590090103

Cited by 14 publications

(12 citation statements)

References 17 publications

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“…To our knowledge, the biochemical mechanism of organotin degradation by a microorganism has not been investigated in de- tail, but microbial degradation of organotin by water samples (16,17), soils (3,22,28), and isolated bacteria (4,23,36) have been demonstrated. To clarify the TPT degradation mechanism by strain CNR15, we prepared a TPT-grown resting-cell suspension and its cell-free culture supernatant and investigated the localization of the TPT degradation activities.…”

Section: Resultsmentioning

confidence: 99%

“…TPT in estuarine water samples was scarcely degraded (15%) during a 60-day incubation (16). Visoottiviseth et al have reported the degradation of TPT by Pseudomonas putida no.C under pure culture conditions (36). While these investigations suggest that microorganisms play an important role in the TPT mineralization in the environment, little is known about the degradation mechanism of TPT and other organotins, and it remains also questionable whether the microbial degradation of organotin is an enzymatic reaction (4).…”

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confidence: 99%

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Degradation of Triphenyltin by a Fluorescent Pseudomonad

Inoue

Takimura

Fuse

et al. 2000

Appl Environ Microbiol

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Triphenyltin (TPT)-degrading bacteria were screened by a simple technique using a post-column highperformance liquid chromatography using 3,3,4,7-tetrahydroxyflavone as a post-column reagent for determination of TPT and its metabolite, diphenyltin (DPT). An isolated strain, strain CNR15, was identified as Pseudomonas chlororaphis on the basis of its morphological and biochemical features. The incubation of strain CNR15 in a medium containing glycerol, succinate, and 130 M TPT resulted in the rapid degradation of TPT and the accumulation of approximately 40 M DPT as the only metabolite after 48 h. The culture supernatants of strain CNR15, grown with or without TPT, exhibited a TPT degradation activity, whereas the resting cells were not capable of degrading TPT. TPT was stoichiometrically degraded to DPT by the solid-phase extract of the culture supernatant, and benzene was detected as another degradation product. We found that the TPT degradation was catalyzed by low-molecular-mass substances (approximately 1,000 Da) in the extract, termed the TPT-degrading factor. The other fluorescent pseudomonads, P. chlororaphis ATCC 9446, Pseudomonas fluorescens ATCC 13525, and Pseudomonas aeruginosa ATCC 15692, also showed TPT degradation activity similar to strain CNR15 in the solid-phase extracts of their culture supernatants. These results suggest that the extracellular low-molecular-mass substance that is universally produced by the fluorescent pseudomonad could function as a potent catalyst to cometabolite TPT in the environment.

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Section: Resultsmentioning

confidence: 99%

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confidence: 99%

Degradation of Triphenyltin by a Fluorescent Pseudomonad

Inoue

Takimura

Fuse

et al. 2000

Appl Environ Microbiol

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“…Microorganisms capable of TBT uptake include certain fungi, viz., Coniophora puteana, Trametes versicolor, Chaetomium globosum, Aureobacidium pullulans, and Cunninghamella elegans (Barug 1981;Orsler and Holland 1982;Gadd et al 1990), bacteria, e.g., Alcaligenes faecalis, Flavobacterium sp., and many Pseudomonas species (Visoottiviseth et al 1994;Kawai et al 1998;Inoue et al 2000Inoue et al , 2003Yamaoka 2003;Roy et al 2004;Roy and Bhosle 2005;Stasinakis et al 2005). Seligman et al 1988 reported that microbial degradation was the primary process for TBT degradation in seawater and determined that the half-life for TBT was approximately 6-7 days.…”

Section: Introductionmentioning

confidence: 99%

Biochemistry of TBT-Degrading Marine Pseudomonads Isolated from Indian Coastal Waters

Sampath

Venkatakrishnan

Ravichandran

et al. 2011

Water Air Soil Pollut

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Tributyltin (TBT) is a very effective biocide and an active ingredient in antifouling paints. Screening along the Indian coast yielded 49 bacterial isolates capable of TBT assimilation. The screening was done based on the ability of bacteria to grow in mineral salt medium (MSM) containing TBT as the sole source of carbon. All the isolates produced exopolysaccharides (biosurfactants) in the medium which aid in emulsification and thus ease bioavailability of TBT. Five isolates were identified as potent TBT degraders (namely, Pseudomonas pseudoalcaligenes, Pseudomonas stutzeri, Pseudomonas mendocina, Pseudomonas putida, and Pseudomonas balearica) based on their biomass production in MSM containing TBT as the sole source of carbon. In addition to evaluating the potential of individual bacterial strains, the study also focused on using a consortium of bacteria to explore their synergistic effect when grown on TBT. Further tests like growth profile, rhamnolipid secretion profile, extracellular protein secretion profile, and detection of siderophores were performed on these isolates when grown in MSM supplemented with 2 mM TBT concentration. Emulsification activity of the crude extracellular polysaccharides against kerosene was evaluated. It can be therefore inferred that TBT degradation by these marine pseudomonads is a twostep process: (a) dispersion of TBT in the aqueous phase and (b) tin-carbon bond cleavage by siderophores affecting debutylation of TBT. The consortium of bacteria may be effective in the treatment of TBT-contaminated waste water in dry docks.

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“…Several strains have been isolated from the sediment or soil, and TPT degradation by these strains was investigated under pure culture conditions (13,24). Although microbe-mediated dealkylation of organotin has been reported, information about details of the mechanism of microbial TPT degradation/ decomposition is still limited (13,14).…”

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Mechanism of Augmentation of Organotin Decomposition by Ferripyochelin: Formation of Hydroxyl Radical and Organotin-Pyochelin-Iron Ternary Complex

Sun

Zhong

2006

Appl Environ Microbiol

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Pyochelin (PCH), a kind of siderophore secreted by Pseudomonas aeruginosa, was recently found to have triphenyltin (TPT)-decomposing capacity. In this work, significant augmentation of TPT decomposition by ferripyochelin (FePCH), the chelating compound of PCH with iron, was demonstrated in Tris-HCl buffer (pH 8.0). The generation of hydroxyl radical (HO·) in the presence of FePCH was observed. Inhibition of HO· generation by adding catalase and HO· scavengers (methanol and dimethyl sulfoxide) decreased TPT decomposition, while an increase in HO· formation in the presence of H 2 O 2 enhanced its decomposition. Our findings indicated that HO· generated in the reaction system was responsible for the enhanced TPT decomposition by FePCH versus PCH. The existence of the TPT-pyochelin-iron ternary complex was demonstrated by electron spray ionization-mass spectrometry, tandem mass spectrometry, and 1 H nuclear magnetic resonance. On the basis of the above results, HO· produced in the presence of FePCH was deduced to be in close proximity to TPT and has more opportunity to attack the Sn-C bond, which resulted in the enhanced organotin decomposition. The information obtained may have considerable environmental significance.Triorganotin compounds, in particular triphenyltin (TPT), have been introduced into the aquatic environment in large quantities due to their extensive use as pesticides in agriculture and as antifouling agents in boat paints (11). As a highly toxic compound, TPT has caused harmful effects on a variety of nontarget organisms, such as mollusks and fish (2, 12), even at aqueous concentrations of a few nanograms per liter (2). Although organotins are banned in most developed countries, they are still being introduced into water and sediment around the world, causing an obvious health risk that has raised public interest in the removal of organotin in and from the environment (14). Microorganisms play a crucial role in organotin removal from the natural environment because of their surprising ability under evolution to degrade xenobiotics (26).Several strains have been isolated from the sediment or soil, and TPT degradation by these strains was investigated under pure culture conditions (13, 24). Although microbe-mediated dealkylation of organotin has been reported, information about details of the mechanism of microbial TPT degradation/ decomposition is still limited (13,14). Walts and Walsh (25) demonstrated that only a few kinds of organotins were substrates for bacterial organomercurial lyase. Most organotin compounds, such as tributyltin and triethyltin, were not substrates for lyase, and some even caused irreversible inhibition of enzymes. Inoue and coworkers (13, 14) have identified a kind of siderophore, pyoverdine (PVD), which was produced by Pseudomonas chlororaphis and was capable of decomposing triphenyltin and tributyltin. We recently showed that pyochelin (PCH), a siderophore secreted by Pseudomonas aeruginosa, could decompose triphenyltin, and its decomposition mechanism was proposed as che...

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Isolation of bacterial culture capable of degrading triphenyltin pesticides

Cited by 14 publications

References 17 publications

Degradation of Triphenyltin by a Fluorescent Pseudomonad

Degradation of Triphenyltin by a Fluorescent Pseudomonad

Biochemistry of TBT-Degrading Marine Pseudomonads Isolated from Indian Coastal Waters

Mechanism of Augmentation of Organotin Decomposition by Ferripyochelin: Formation of Hydroxyl Radical and Organotin-Pyochelin-Iron Ternary Complex

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