Isolation of Bacterial Strain with Xylanase and Xylose/Glucose Isomerase (GI) Activity and Whole Cell Immobilization for Improved Enzyme Production

Sharma, Hem Kanta; Xu, Chunbao; Qin, Wensheng

doi:10.1007/s12649-020-01013-5

Cited by 10 publications

(3 citation statements)

References 43 publications

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“…In a further, SDS profiles indicated that the molecular weight of lactase was about 120 kDa and that of glucose isomerase was about 70 kDa. The relative molecular mass of glucose isomerase reported in the relevant literature was about 63 kDa [ 62 ] and the molecular weight of lactase was between about 60 and 120 kDa [ 61 , 63 ], similar to the results we obtained.…”

Section: Resultssupporting

confidence: 90%

Co-Immobilization of Lactase and Glucose Isomerase on the Novel g-C3N4/CF Composite Carrier for Lactulose Production

et al. 2022

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The g-C3N4/CF composite carrier was prepared by ultrasound-assisted maceration and high-temperature calcination. The enzyme immobilization using the g-C3N4/CF as the novel carrier to immobilize lactase and glucose isomerase was enhanced for lactulose production. The carbon fiber (CF) was mixed with melamine powder in the mass ratio of 1:8. The g-C3N4/CF composite carrier was obtained by calcination at 550 °C for 3 h. After the analysis of characteristics, the g-C3N4/CF was successfully composited with the carbon nitride and CF, displaying the improvement of co-immobilization efficiency with the positive effects on the stability of the enzyme. The immobilization efficiency of the co-immobilized enzyme was 37% by the novel carrier of g-C3N4/CF, with the enzyme activity of 13.89 U g−1 at 60 °C. The relative activities of co-immobilized enzymes maintained much more steadily at the wider pH and higher temperature than those of the free dual enzymes, respectively. In the multi-batches of lactulose production, the relative conversion rates in enzymes co-immobilized by the composite carrier were higher than that of the free enzymes during the first four batches, as well as maintaining about a 90% relative conversation rate after the sixth batch. This study provides a novel method for the application of g-C3N4/CF in the field of immobilizing enzymes for the production of lactulose.

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Section: Resultssupporting

confidence: 90%

Co-Immobilization of Lactase and Glucose Isomerase on the Novel g-C3N4/CF Composite Carrier for Lactulose Production

et al. 2022

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“…SDS-PAGE results of SmGI used in the experiment are shown in Figure 1a. The main bind at approximately 60 kD was detected, which is consistent with published reports [38]. Also, the purity of the protein bind showed that the protein was suitable for further experiments.…”

Section: Discussionsupporting

confidence: 91%

Enhanced High-Fructose Corn Syrup Production: Immobilizing Serratia marcescens Glucose Isomerase on MOF (Co)-525 Reduces Co2+ Dependency in Glucose Isomerization to Fructose

Geng,

Li,

Wang

et al. 2024

Foods

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The escalating demand for processed foods has led to the widespread industrial use of glucose isomerase (GI) for high-fructose corn syrup (HFCS) production. This reliance on GIs necessitates continual Co2+ supplementation to sustain high catalytic activity across multiple reaction cycles. In this study, Serratia marcescens GI (SmGI) was immobilized onto surfaces of the metal-organic framework (MOF) material MOF (Co)-525 to generate MOF (Co)-525-GI for use in catalyzing glucose isomerization to generate fructose. Examination of MOF (Co)-525-GI structural features using scanning electron microscopy-energy dispersive spectroscopy, Fourier-transform infrared spectroscopy, and ultraviolet spectroscopy revealed no structural changes after SmGI immobilization and the addition of Co2+. Notably, MOF (Co)-525-GI exhibited optimal catalytic activity at pH 7.5 and 70 °C, with a maximum reaction rate (Vmax) of 37.24 ± 1.91 μM/min and Km value of 46.25 ± 3.03 mM observed. Remarkably, immobilized SmGI exhibited sustained high catalytic activity over multiple cycles without continuous Co2+ infusion, retaining its molecular structure and 96.38% of its initial activity after six reaction cycles. These results underscore the potential of MOF (Co)-525-GI to serve as a safer and more efficient immobilized enzyme technology compared to traditional GI-based food-processing technologies.

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“…Glucose/xylose isomerase reversely converts D-glucose and D-Xylose into D-fructose and D-Xylulose, respectively [148,149]. Though glucose/xylose isomerase was extensively reported from many bacteria as Bacillus licheniformis [147], Serratia marcescens [150], Caldicellulosiruptor bescii [151], and Streptomyces lividans [152], the fungal enzyme was reported from a few species of Aspergillus genus [153]. Marshall and Kooi reported for the first time the ability of xylose isomerase from Pseudomonas hydrophila to convert D-glucose into D-fructose [154].…”

Section: Isomerases (Ec 5)mentioning

confidence: 99%

A Comprehensive Insight into Fungal Enzymes: Structure, Classification, and Their Role in Mankind’s Challenges

El‐Gendi

Saleh

Badierah

et al. 2021

JoF

122

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Enzymes have played a crucial role in mankind’s challenges to use different types of biological systems for a diversity of applications. They are proteins that break down and convert complicated compounds to produce simple products. Fungal enzymes are compatible, efficient, and proper products for many uses in medicinal requests, industrial processing, bioremediation purposes, and agricultural applications. Fungal enzymes have appropriate stability to give manufactured products suitable shelf life, affordable cost, and approved demands. Fungal enzymes have been used from ancient times to today in many industries, including baking, brewing, cheese making, antibiotics production, and commodities manufacturing, such as linen and leather. Furthermore, they also are used in other fields such as paper production, detergent, the textile industry, and in drinks and food technology in products manufacturing ranging from tea and coffee to fruit juice and wine. Recently, fungi have been used for the production of more than 50% of the needed enzymes. Fungi can produce different types of enzymes extracellularly, which gives a great chance for producing in large amounts with low cost and easy viability in purified forms using simple purification methods. In the present review, a comprehensive trial has been advanced to elaborate on the different types and structures of fungal enzymes as well as the current status of the uses of fungal enzymes in various applications.

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Isolation of Bacterial Strain with Xylanase and Xylose/Glucose Isomerase (GI) Activity and Whole Cell Immobilization for Improved Enzyme Production

Cited by 10 publications

References 43 publications

Co-Immobilization of Lactase and Glucose Isomerase on the Novel g-C3N4/CF Composite Carrier for Lactulose Production

Co-Immobilization of Lactase and Glucose Isomerase on the Novel g-C3N4/CF Composite Carrier for Lactulose Production

Enhanced High-Fructose Corn Syrup Production: Immobilizing Serratia marcescens Glucose Isomerase on MOF (Co)-525 Reduces Co2+ Dependency in Glucose Isomerization to Fructose

A Comprehensive Insight into Fungal Enzymes: Structure, Classification, and Their Role in Mankind’s Challenges

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