Isolation of bacteriophages and their application to control Pseudomonas aeruginosa in planktonic and biofilm models

Kwiatek, Magdalena; Parasion, Sylwia; Rutyna, Paweł; Mizak, Lidia; Gryko, Romuald; Niemcewicz, Marcin; Olender, Alina; Łobocka, Małgorzata

doi:10.1016/j.resmic.2016.10.009

Cited by 55 publications

(38 citation statements)

References 75 publications

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“…The best dilution in all three isolates was MOI 0.01(0 6 pfu/ml). Similar to other studies, this study showed that the isolated lytic phage had an impact on the bio lm of Acinetobacter baumannii [20,22,23,34]. The use of phage has been proposed as an alternative method due to the high drug resistance of Acinetobacter baumannii and bacterial adaptation at hospital levels.…”

Section: Discussionsupporting

confidence: 84%

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Efficacy of Isolated Bacteriophage Against Biofilm Embedded Colistin-Resistant Acinetobacter baumannii

Ebrahimi

Sisakhtpour

Mirzaei

et al. 2020

Preprint

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Objective: Acinetobacter baumannii is responsible for most nosocomial infections in hospitals. It has the ability to form biofilms and has a high degree of antibiotic resistance. Colistin is one of the last therapeutic options for the treatment of Multi Drug Resistance infections. Recently, strains of this pathogen resistance to the colistin were reported increasingly. Therefore, alternative antibacterial methods such as phage therapy are being researched. Results: From 15 MDR A. baumannii clinical isolates, 26.6% were resistant to colistin, 80% were able to produce strong biofilm, and 20% produce weak biofilm. The isolated lytic phage (IsfAB78) was able to reduce the biofilm by up to 87%. Since most of the MDR colistin-resistant strains produce biofilm, and MDR A. baumannii infections are difficult to treat, development of phage therapy could be an alternative in the future. Phage IsfAB78 is a good candidate for this purpose.

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Section: Discussionsupporting

confidence: 84%

“…baumannii. Unfortunately, the increasing use of colistin has resulted in the emergence of resistance as well [22][23][24][25]. Therefore, looking for an alternative therapeutic method is imperative.…”

Section: Discussionmentioning

confidence: 99%

Efficacy of Isolated Bacteriophage Against Biofilm Embedded Colistin-Resistant Acinetobacter baumannii

Ebrahimi

Sisakhtpour

Mirzaei

et al. 2020

Preprint

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“…Some studies relied exclusively on PAO1, while other studies focused on clinical isolates or other reference strains. Notably, eight out of 34 studies [ 27 , 28 , 29 , 30 , 31 , 32 , 33 , 34 ] used both clinical and reference strains ( Supplemental Table S4 ). Clearly, reference strains allow comparison of phage performance across laboratories.…”

Section: Discussionmentioning

confidence: 99%

“…Clearly the most rapid reduction was always achieved with the highest MOI, however, with phages SL1 and SL2 suppression of bacteria was possible even at the lowest MOI of 10 −6 . We are not aware of a study reporting a successful application of MOIs < 10 −4 against MDR-PA strains; for instance, Henry et al (2013) used a MOI of 10 −3 [ 41 ], while otherwise typical MOIs used in liquid infection assays against P. aeruginosa range somewhere between 1 and 10 −2 [ 28 , 42 , 43 , 44 , 45 , 46 ] or higher [ 47 ]. In light of the concerns that the patient’s immune system may eliminate or inactivate a substantial proportion of administered therapeutic phages before they enter the site of infection [ 48 , 49 ], an extant effectivity at very low MOIs is reassuring, as it still argues for the functioning of phage therapy as long as a few intact virions reach their target host.…”

Section: Discussionmentioning

confidence: 99%

Differential Effect of Newly Isolated Phages Belonging to PB1-Like, phiKZ-Like and LUZ24-Like Viruses against Multi-Drug Resistant Pseudomonas aeruginosa under Varying Growth Conditions

Latz

Krüttgen

Häfner

et al. 2017

Viruses

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In this study, we characterize three phages (SL1 SL2, and SL4), isolated from hospital sewage with lytic activity against clinical isolates of multi-drug resistant Pseudomonas aeruginosa (MDR-PA). The host spectrum ranged from 41% to 54%, with all three phages together covering 79% of all tested clinical isolates. Genome analysis revealed that SL1 (65,849 bp, 91 open reading frames ORFs) belongs to PB1-like viruses, SL2 (279,696 bp, 354 ORFs) to phiKZ-like viruses and SL4 (44,194 bp, 65 ORFs) to LUZ24-like viruses. Planktonic cells of four of five selected MDR-PA strains were suppressed by at least one phage with multiplicities of infection (MOIs) ranging from 1 to 10−6 for 16 h without apparent regrowth of bacterial populations. While SL2 was most potent in suppressing planktonic cultures the strongest anti-biofilm activity was observed with SL4. Phages were able to rescue bacteria-infected wax moth larvae (Galleria melonella) for 24 h, whereby highest survival rates (90%) were observed with SL1. Except for the biofilm experiments, the effect of a cocktail with all three phages was comparable to the action of the best phage alone; hence, there are no synergistic but also no antagonistic effects among phages. The use of a cocktail with these phages is therefore expedient for increasing host range and minimizing the development of phage resistance.

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“…In general, phages tend to be more stable at a wide range of pHs than at different temperatures, in fact high temperatures cause the loss of phage viability (Jun et al, 2014;Kwiatek et al, 2017;Peng and Yuan, 2018), although Malek et al (2009) reported opposite results for 3 rhizophages ( RP1, RP2, and RP3) specific for Robinia pseudoacacia which were stable at 37 • C and very sensitive at 4 • C.…”

Section: Thermal Ph and Salt Stabilitymentioning

confidence: 99%

Diversity of Sinorhizobium (Ensifer) meliloti Bacteriophages in the Rhizosphere of Medicago marina: Myoviruses, Filamentous and N4-Like Podovirus

et al. 2020

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Using different Sinorhizobium meliloti strains as hosts, we isolated eight new virulent phages from the rhizosphere of the coastal legume Medicago marina. Half of the isolated phages showed a very narrow host range while the other half exhibited a wider host range within the strains tested. Electron microscopy studies showed that phages M_ort18, M_sf1.2, and M_sf3.33 belonged to the Myoviridae family with feature long, contractile tails and icosaedral head. Phages I_sf3.21 and I_sf3.10T appeared to have filamentous shape and produced turbid plaques, which is a characteristic of phages from the Inoviridae family. Phage P_ort11 is a member of the Podoviridae, with an icosahedral head and a short tail and was selected for further characterization and genome sequencing. P_ort11 contained linear, double-stranded DNA with a length of 75239 bp and 103 putative open reading frames. BLASTP analysis revealed strong similarities to Escherichia phage N4 and other N4-like phages. This is the first report of filamentous and N4-like phages that infect S. meliloti.

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Isolation of bacteriophages and their application to control Pseudomonas aeruginosa in planktonic and biofilm models

Cited by 55 publications

References 75 publications

Efficacy of Isolated Bacteriophage Against Biofilm Embedded Colistin-Resistant Acinetobacter baumannii

Efficacy of Isolated Bacteriophage Against Biofilm Embedded Colistin-Resistant Acinetobacter baumannii

Differential Effect of Newly Isolated Phages Belonging to PB1-Like, phiKZ-Like and LUZ24-Like Viruses against Multi-Drug Resistant Pseudomonas aeruginosa under Varying Growth Conditions

Diversity of Sinorhizobium (Ensifer) meliloti Bacteriophages in the Rhizosphere of Medicago marina: Myoviruses, Filamentous and N4-Like Podovirus

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