Isolation of Beauveria Strains and Their Potential as Control Agents for Lema bilineata Germar (Coleoptera: Chrysomelidae)

Furuie, Jason Lee; Stuart, Andressa Katiski da Costa; Voidaleski, Morgana Ferreira; Zawadneak, María Aparecida Cassilha; Pimentel, Ida Chapaval

doi:10.3390/insects13010093

Cited by 4 publications

(1 citation statement)

References 15 publications

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“…They can also avoid ecological balance and provide another safe, economical and effective option. For example, Beauveria bassiana has been used as an environmentally friendly alternative chemical insecticide ( Gao et al, 2011 ; Furuie et al, 2022 ). As mentioned above, the importance of fungi as microbial control agents has created great interest in the regulation of the formation of these products.…”

Section: Introductionmentioning

confidence: 99%

Whole-genome DNA methylome analysis of different developmental stages of the entomopathogenic fungus Beauveria bassiana NCHU-157 by nanopore sequencing

Chang²,

Yen³

et al. 2023

Front. Genet.

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The entomopathogenic fungus (EPF), Beauveria bassiana, is an important and commonly used EPF for microbial control. However, the role of DNA methylation has not been thoroughly studied. Therefore, the whole genomic DNA methylome of one promising EPF isolate, B. bassiana NCHU-157 (Bb-NCHU-157), was investigated by Oxford Nanopore Technologies (ONT). First, the whole genome of Bb-NCHU-157 was sequenced by next-generation sequencing (NGS) and ONT. The genome of Bb-NCHU-157 contains 16 contigs with 34.19 Mb and 50% GC content, which are composed of 10,848 putative protein-coding genes. Two putative DNA methyltransferases (DNMTs) were found, including Dim-2 and C-5 cytosine-specific DNA methylases. Both DNMTs showed higher expression levels in the mycelium stage than in the conidia stage, indicating that development of DNA methylation in Bb-NCHU-157 might occur in the mycelium stage. The global methylation level of the mycelium stage (5 mC = 4.56%, CG = 3.33%, CHG = 0.74%, CHH = 0.49%) was higher than that of the conidial stage (5 mC = 2.99%, CG = 1.99%, CHG = 0.63%, CHH = 0.37%) in both the gene and transposable element (TE) regions. Furthermore, the TE regions showed higher methylation frequencies than the gene regions, especially for CHH site methylation, suggesting regulation of genomic stabilization during mycelium development. In the gene regions, high methylation frequencies were found around the transcription start site (TSS) and transcription end site (TES). Moreover, CG and CHG methylation mainly occur in the promoter and intergenic regions, while CHH methylation occurs in the TE region. Among the methylated regions, 371, 661, and 756 differentially DNA methylated regions (DMRs) were hypermethylated in the mycelium in CG, CHG, and CHH, while only 13 and 7 DMRs were hypomethylated in the mycelium in CHG, and CHH, respectively. Genes located in the DMR shared the GO terms, DNA binding (GO: 0003677), and sequence-specific DNA binding (GO: 0043565) for hypermethylation in the mycelium, suggesting that methylation might regulate gene expression from the initial process. Evaluation of the DNA methylome in Bb-NCHU-157 by ONT provided new insight into this field. These data will be further validated, and epigenetic regulation during the development of B. bassiana will be explored.

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Section: Introductionmentioning

confidence: 99%

Whole-genome DNA methylome analysis of different developmental stages of the entomopathogenic fungus Beauveria bassiana NCHU-157 by nanopore sequencing

Chang²,

Yen³

et al. 2023

Front. Genet.

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Potential of microbial control agents in a strategy against Duponchelia fovealis Zeller (Lepidoptera: Crambidae)

Da Luz,

Poitevin,

Zimmermann

et al. 2024

Crop Protection

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First report of Beauveria bassiana in the in vivo control of Eriosoma lanigerum in Brazilian apple trees

Matsumura,

Silva

et al. 2023

Rev. Ceres

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This study was conducted in a commercial apple orchard (Malus domestica) with high woolly apple aphid (Eriosoma lanigerum) incidence, which is highly harmful to apple culture and infects the trunk, branch, roots, shoot, and fruit.Although the fungus Beauveria bassiana is widely reported as a control agent, there is no evidence of its control action against this pest. This case study was conducted under natural infestation conditions to evaluate the effective action of B. bassiana in E. lanigerum nymph mortality and aimed to evaluate the control potential of E. lanigerum treated with different formulation doses based on B. bassiana strain ICBBb252. The experimental unit consisted of ten plants in four randomized blocks, where one colony per plant was previously marked in an orchard (2500 plants/ha). The treatments consisted of applying the formulate in 100, 200, and 300 mL/ha dosages at a 10 9 spore/mL concentration and one control group; 10, 20, 30, and 40 days after application, the treatments were evaluated by counting the number of colonies alive.The control of woolly apple aphids by B. bassiana strain ICBBb252 was more effective at 200 and 300 mL/ha dosages and has no phytotoxic effects on plants.

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Isolation of Beauveria Strains and Their Potential as Control Agents for Lema bilineata Germar (Coleoptera: Chrysomelidae)

Cited by 4 publications

References 15 publications

Whole-genome DNA methylome analysis of different developmental stages of the entomopathogenic fungus Beauveria bassiana NCHU-157 by nanopore sequencing

Whole-genome DNA methylome analysis of different developmental stages of the entomopathogenic fungus Beauveria bassiana NCHU-157 by nanopore sequencing

Potential of microbial control agents in a strategy against Duponchelia fovealis Zeller (Lepidoptera: Crambidae)

First report of Beauveria bassiana in the in vivo control of Eriosoma lanigerum in Brazilian apple trees

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