Isolation of bioactive molecule from Indian hog plum (Spondias mangifera Willd.) fruit

Abstract

Fresh Indian hog plum fruits (Spondias mangifera Willd.) were sliced, dried and powdered. Sequential extraction of this fruit powder was carried out using hexane, chloroform, ethylacetate and methanol. When these extracts were tested for different bioactivities, methanol extract showed the highest antioxidant activity and this extract was used to extract the bioactive molecule by silica gel column chromatography for isolating the pure bioactive molecule. The structure of the bioactive molecule was elucidated by analyzing UV, IR, LC-MS and 2D-HMQCT NMR spectral data and named it as Spondiol. This bioactive molecule showed an EC50 value of 80, 104, 161 and 196 for DPPH radical scavenging activity, Superoxide radical scavenging activity, Lipid peroxidation inhibitory activity and metal chelating activity respectively. Spondiol also inhibited platelet aggregation with IC50 of 250 mg and 350 mg when induced by collagen and Adenosine Diphosphate (ADP) respectively. It also controlled the growth of bacteria viz., b. subtilis, M. luteus, S. fecalis, S. aureus, S. typhi, S. dysenteriae, K. pneumoniae and S. aureus.