Isolation of Biogenic Amines Using Paramagnetic Microparticles Off-Line Coupled with Ion Exchange Liquid Chromatography

Cernei, Natalia; Heger, Zbyněk; Kopel, Pavel; Skládanka, Jiří­; Zítka, Ondřej; Adam, Vojtěch; Kizek, René

doi:10.1007/s10337-014-2731-8

Cited by 9 publications

(12 citation statements)

References 32 publications

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“…The surface of paramagnetic iron oxide particles can be easily modified to provide binding sites for various kinds of molecules and due to their iron component they can be easily immobilized using an external magnetic field . Therefore, these structures were used as a platform for magnetic immobilization of influenza A virus particles and their nucleic acid after lysis.…”

Section: Resultsmentioning

confidence: 99%

A two‐step protocol for isolation of influenza A (H7N7) virions and their RNA for PCR diagnostics based on modified paramagnetic particles

et al. 2016

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Annual epidemics of influenza cause death of hundreds of thousands people and they also have a significant economic impact. Hence, a need for fast and cheap influenza diagnostic method is arising. The conventional methods for an isolation of the viruses are time-consuming and require expensive instrumentation as well as trained personnel. In this study, we modified the surface of nanomaghemite (γ-Fe2 O3 ) paramagnetic core with tetraethyl orthosilicate and (3-aminopropyl)triethoxysilane and the resulting particles were utilized for the isolation of H7N7 influenza virions. Consequently, we designed γ-Fe2 O3 paramagnetic core modified with calcium tripolyphosphate which was employed for the isolation of viral nucleic acid after virion's lysis. Both of these procedures can be performed rapidly in less than 10 min and, in combination with the RT-PCR, the whole influenza detection can be shortened to few hours. Moreover, the whole protocol could be easily automated and/or miniaturized, and thus can serve as a basis for use in a lab-on-a-chip device. We assume that magnetic isolation is an exceptional procedure which can significantly accelerate the diagnostic possibilities of a broad spectrum of diseases.

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Section: Resultsmentioning

confidence: 99%

A two‐step protocol for isolation of influenza A (H7N7) virions and their RNA for PCR diagnostics based on modified paramagnetic particles

et al. 2016

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“…The superparamagnetic iron oxide nanoparticles (SPIONs ‐ γ‐Fe 2 O 3 and Fe 3 O 4 ) are versatile nanoscaled materials that have been intensively examined for numerous applications . They can be simply functionalized to provide binding sites toward various molecules and moreover, they can be conveniently immobilized via external magnetic field.…”

Section: Resultsmentioning

confidence: 99%

Bioconjugation of peptides using advanced nanomaterials to examine their interactions in 3D printed flow‐through device

et al. 2015

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Peptide-peptide interactions are crucial in the living cell as they lead to the formation of the numerous types of complexes. In this study, synthetic peptides containing 11 of cysteines (α-domain of metallothionein (MT)) and sialic acid binding region (130-loop of hemagglutinin (HA)) were employed. The aim of the experiment was studying the interactions between MT and HA-derived peptides. For this purpose, fragments were tagged with cysteines at C-terminal part to serve as ligand sites for PbS and CuS quantum dots (QDs), and therefore these conjugates can be traced and quantified during wide spectrum of methods. As a platform for interaction, γ-Fe2O3 paramagnetic particles modified with tetraethyl orthosilicate and (3-aminopropyl)triethoxysilane (hydrodynamic diameter 30-40 nm) were utilized and MT/HA interactions were examined using multi-instrumental approach including electrochemistry, electrophoretic methods, and MALDI-TOF/TOF mass spectrometry. It was found that peptides enter mutual creation of complexes, which are based on some of nonbonded interactions. The higher willingness to interact was observed in MT-derived peptides toward immobilized HA. Finally, we designed and manufactured flow-through electrochemical 3D printed device (reservoir volume 150 μL) and utilized it for automated analysis of the HA/MT metal labels. Under the optimal conditions, (deposition time and flow rate 80 s and 1.6 mL/min for CuS and 120 s and 1.6 mL/min PbS, respectively), the results of peptide-conjugated QDs were comparable with atomic absorption spectrometry.

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“…The elution mobile phase (pH 2.7) contained 11.11 g of citric acid, 4.04 g of sodium citrate, 9.25 g of NaCl, 0.1 g of sodium azide and 2.5 mL of thiodiglycol per liter of solution, using the flow rate of 0.25 mL·min −1 . Other experimental conditions were used as previously published [ 31 ].…”

Section: Methodsmentioning

confidence: 99%

Study of Linkage between Glutathione Pathway and the Antibiotic Resistance of Escherichia coli from Patients’ Swabs

Komínková

Michálek

Číhalová

et al. 2015

IJMS

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In this work, we focused on the differences between bacterial cultures of E. coli obtained from swabs of infectious wounds of patients compared to laboratory E. coli. In addition, blocking of the protein responsible for the synthesis of glutathione (γ-glutamylcysteine synthase—GCL) using 10 mM buthionine sulfoximine was investigated. Each E. coli showed significant differences in resistance to antibiotics. According to the determined resistance, E. coli were divided into experimental groups based on a statistical evaluation of their properties as more resistant and more sensitive. These groups were also used for finding the differences in a dependence of the glutathione pathway on resistance to antibiotics. More sensitive E. coli showed the same kinetics of glutathione synthesis while blocking GCL (Km 0.1 µM), as compared to non-blocking. In addition, the most frequent mutations in genes of glutathione synthetase, glutathione peroxidase and glutathione reductase were observed in this group compared to laboratory E.coli. The group of “more resistant” E. coli exhibited differences in Km between 0.3 and 0.8 µM. The number of mutations compared to the laboratory E. coli was substantially lower compared to the other group.

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Isolation of Biogenic Amines Using Paramagnetic Microparticles Off-Line Coupled with Ion Exchange Liquid Chromatography

Cited by 9 publications

References 32 publications

A two‐step protocol for isolation of influenza A (H7N7) virions and their RNA for PCR diagnostics based on modified paramagnetic particles

A two‐step protocol for isolation of influenza A (H7N7) virions and their RNA for PCR diagnostics based on modified paramagnetic particles

Bioconjugation of peptides using advanced nanomaterials to examine their interactions in 3D printed flow‐through device

Study of Linkage between Glutathione Pathway and the Antibiotic Resistance of Escherichia coli from Patients’ Swabs

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