Isolation of Borrelia burgdorferi from arthropods Collected in Texas

Teltow, Glenna J.; Fournier, Paul V.; Rawlings, Julie A.

doi:10.4269/ajtmh.1991.44.469

Cited by 51 publications

(33 citation statements)

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“…have been largely refractory to the culture medium that supports B. burgdorferi. B. lonestari has never been cultured (3,9), and few instances of culture of B. burgdorferi from A. americanum have been reported in the literature (5,23,29,34,38). The relationship of B. lonestari to its tick and vertebrate hosts, to other Borrelia spp., and to human disease awaits explanation.…”

Section: Discussionmentioning

confidence: 99%

Evidence ofBorrelia lonestariDNA inAmblyomma americanum(Acari: Ixodidae) Removed from Humans

et al. 2003

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Section: Discussionmentioning

confidence: 99%

Evidence ofBorrelia lonestariDNA inAmblyomma americanum(Acari: Ixodidae) Removed from Humans

et al. 2003

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“…5 The increasing heterogeneity of B burgdorferi in North America may relate to the possibility that strain variants may have adapted to the Lone Star tick. [6][7][8][9] Additionally, the identification of Borrelia lonestari in Lone Star ticks 10 and the findings of other researchers of spirochetes consistent with B burgdorferi or Borrelia DNA in Lone Star ticks 8,[11][12][13][14][15] have warranted a closer look at Lone Star ticks as a possible vector of a borreliosis. The exact cause of physician-diagnosed erythema migrans in the South, including Missouri, is currently being investigated.…”

Section: Discussionmentioning

confidence: 99%

Physician-Diagnosed Erythema Migrans and Erythema Migrans–like Rashes Following Lone Star Tick Bites

Masters¹,

Granter²,

Duray³

et al. 1998

Arch Dermatol

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“…Transverse sections of spirochetes revealed a trilaminar membrane consisting of an outer membrane (sheath), a cell wall, and an inner cytoplasmic membrane, analogous to what has been described for other Borrelia spp. (7,49) (Fig. 3C and 5C).…”

Section: Culture Isolationmentioning

confidence: 99%

“…A. americanum has been shown to be an incompetent vector for B. burgdorferi, and serologic testing of STARI patients does not support a diagnosis of classic Lyme disease despite microscopic evidence of spirochetes in biopsy samples of affected skin, leading researchers to speculate that another Borrelia sp. may be responsible (16,24,29,31,33,35,39,42,48,49). However, all attempts to culture spirochetes from patients with STARI and from ticks have failed.…”

mentioning

confidence: 99%

First Culture Isolation of Borrelia lonestari , Putative Agent of Southern Tick-Associated Rash Illness

Varela¹,

Luttrell

Howerth³

et al. 2004

J Clin Microbiol

108

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Southern tick-associated rash illness (STARI) is a Lyme disease-like infection described in patients in the southeastern and south-central United States, where classic Lyme disease is relatively rare. STARI develops following the bite of a lone star tick (Amblyomma americanum) and is thought to be caused by infection with an "uncultivable" spirochete tentatively named Borrelia lonestari. In this study, wild lone star ticks collected from an area where B. lonestari is endemic were cocultured in an established embryonic tick cell line (ISE6). The cultures were examined by dark-field microscopy for evidence of infection, and spirochete identity and morphology were evaluated by flagellin B and 16S rRNA gene sequence, by reaction to Borrelia-wide and B. burgdorferi-specific monoclonal antibodies, and by electron microscopy. Live spirochetes were first visualized in primary culture of A. americanum ticks by dark-field microscopy 14 days after the cell culture was inoculated. The sequences of the flagellin B and 16S rRNA genes of cultured spirochetes were consistent with previously reported sequences of B. lonestari. The cultured spirochetes reacted with a Borrelia-wide flagellin antibody, but did not react with an OspA antibody specific to B. burgdorferi, by indirect fluorescent antibody testing. Electron microscopy demonstrated organisms that were free and associated with ISE6 cells, with characteristic Borrelia sp. morphology. This study describes the first successful isolation of B. lonestari in culture, providing a much needed source of organisms for the development of diagnostic assays and forming a basis for future studies investigating the role of the organism as a human disease agent.

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Isolation of Borrelia burgdorferi from arthropods Collected in Texas

Cited by 51 publications

References 0 publications

Evidence ofBorrelia lonestariDNA inAmblyomma americanum(Acari: Ixodidae) Removed from Humans

Evidence ofBorrelia lonestariDNA inAmblyomma americanum(Acari: Ixodidae) Removed from Humans

Physician-Diagnosed Erythema Migrans and Erythema Migrans–like Rashes Following Lone Star Tick Bites

First Culture Isolation of Borrelia lonestari , Putative Agent of Southern Tick-Associated Rash Illness

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