Isolation of canine parvovirus from a cat manifesting clinical signs of feline panleukopenia

Mochizuki, Masahito; Horiuchi, Motohiro; Hiragi, H; Gabriel, M C San; Yasuda, Naoki; Uno, Tsukasa

doi:10.1128/jcm.34.9.2101-2105.1996

Cited by 122 publications

(88 citation statements)

References 19 publications

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“…The pathogenicity of CPV variants for cats is not fully understood. Some studies have suggested that CPV had the same pathogenic potential as FPV in cats [3,[32][33][34][35]; in other studies, clinical signs were not observed in infected animals, with the exception of transient leukopenia [36,37]. These results led to the speculation that CPV, compared to FPV, can most frequently cause asymptomatic and persistent infection in cats, even if additional studies are clearly needed to fully understand the potential of cats as CPV carriers.…”

Section: Discussionmentioning

confidence: 99%

Molecular analysis of carnivore Protoparvovirus detected in white blood cells of naturally infected cats

et al. 2018

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BackgroundCats are susceptible to feline panleukopenia virus (FPV) and canine parvovirus (CPV) variants 2a, 2b and 2c. Detection of FPV and CPV variants in apparently healthy cats and their persistence in white blood cells (WBC) and other tissues when neutralising antibodies are simultaneously present, suggest that parvovirus may persist long-term in the tissues of cats post-infection without causing clinical signs. The aim of this study was to screen a population of 54 cats from Sardinia (Italy) for the presence of both FPV and CPV DNA within buffy coat samples using polymerase chain reaction (PCR). The DNA viral load, genetic diversity, phylogeny and antibody titres against parvoviruses were investigated in the positive cats.ResultsCarnivore protoparvovirus 1 DNA was detected in nine cats (16.7%). Viral DNA was reassembled to FPV in four cats and to CPV (CPV-2b and 2c) in four cats; one subject showed an unusually high genetic complexity with mixed infection involving FPV and CPV-2c. Antibodies against parvovirus were detected in all subjects which tested positive to DNA parvoviruses.ConclusionsThe identification of FPV and CPV DNA in the WBC of asymptomatic cats, despite the presence of specific antibodies against parvoviruses, and the high genetic heterogeneity detected in one sample, confirmed the relevant epidemiological role of cats in parvovirus infection.

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Section: Discussionmentioning

confidence: 99%

Molecular analysis of carnivore Protoparvovirus detected in white blood cells of naturally infected cats

et al. 2018

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“…Two nested PCRs amplified a 1,746 nucleotide segment. The external PCR amplified a 2,401 nucleotide segment and was performed by combining the primers VPF and M5mod (Mochizuki, Horiuchi, & Hiragi, 1996;Steinel et al, 2000); whereas the internal PCR was conducted using the primers P1 and VPR (Battilani et al, 2001;Mochizuki, San Gabriel, Nakatani, Yoshida, & Harasawa, 1993) (Table 2). The temperature profile for the external PCR was set at 94°C for 5′, followed by 45 cycles: 94°C for 30″, 55°C for 30″ and 72°C for 2′30″, with a final extension of 72°C for 7′.…”

Section: Molecular Analysismentioning

confidence: 99%

Genetic characterization of Carnivore Parvoviruses in Spanish wildlife reveals domestic dog and cat‐related sequences

Calatayud

Esperón²,

Velarde

et al. 2019

Transbound Emerg Dis

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The impact of carnivore parvovirus infection on wild populations is not yet understood; disease signs are mainly developed in pups and assessing the health of litters in wild carnivores has big limitations. This study aims to shed light on the virus dynamics among wild carnivores thanks to the analysis of 213 samples collected between 1994 and 2013 in wild ecosystems from Spain. We determined the presence of carnivore parvovirus DNA by real‐time PCR and sequenced the vp2 gen from 22 positive samples to characterize the strains and to perform phylogenetic analysis. The presence of carnivore parvovirus DNA was confirmed in 18% of the samples, with a higher prevalence detected in wolves (Canis lupus signatus, 70%). Fourteen sequences belonging to nine wolves, three Eurasian badgers (Meles meles), a common genet (Genetta genetta) and a European wildcat (Felis silvestris) were classified as canine parvovirus 2c (CPV‐2c); five sequences from three wolves, a red fox (Vulpes vulpes) and a stone marten (Martes foina) as CPV‐2b; and three sequences from a badger, a genet and a stone marten as feline parvovirus (FPV). This was the first report of a wildcat infected with a canine strain. Sequences described in this study were identical or very close related to others previously found in domestic carnivores from distant countries, suggesting that cross‐species transmission takes place and that the parvovirus epidemiology in Spain, as elsewhere, could be influenced by global factors.

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“…The molecular characterization of parvovirus from ten mongooses, three common genets, three badgers, three stone martens and eight red foxes was endeavored through amplification of the complete vp2 gene (approximately 1.9 kb), attempted by using different combinations of primers described by [25,50,51,52] ( Table 3).…”

Section: Molecular Characterization Of Parvovirusmentioning

confidence: 99%

Snapshot of Viral Infections in Wild Carnivores Reveals Ubiquity of Parvovirus and Susceptibility of Egyptian Mongoose to Feline Panleukopenia Virus

et al. 2013

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The exposure of wild carnivores to viral pathogens, with emphasis on parvovirus (CPV/FPLV), was assessed based on the molecular screening of tissue samples from 128 hunted or accidentally road-killed animals collected in Portugal from 2008 to 2011, including Egyptian mongoose (Herpestes ichneumon, n = 99), red fox (Vulpes vulpes, n = 19), stone marten (Martes foina, n = 3), common genet (Genetta genetta, n = 3) and Eurasian badger (Meles meles, n = 4). A high prevalence of parvovirus DNA (63%) was detected among all surveyed species, particularly in mongooses (58%) and red foxes (79%), along with the presence of CPV/FPLV circulating antibodies that were identified in 90% of a subset of parvovirus-DNA positive samples. Most specimens were extensively autolysed, restricting macro and microscopic investigations for lesion evaluation. Whenever possible to examine, signs of active disease were not present, supporting the hypothesis that the parvovirus vp2 gene fragments detected by real-time PCR possibly correspond to viral DNA reminiscent from previous infections. The molecular characterization of viruses, based on the analysis of the complete or partial sequence of the vp2 gene, allowed typifying three viral strains of mongoose and four red fox’s as feline panleukopenia virus (FPLV) and one stone marten’s as newCPV-2b type. The genetic similarity found between the FPLV viruses from free-ranging and captive wild species originated in Portugal and publicly available comparable sequences, suggests a closer genetic relatedness among FPLV circulating in Portugal.Although the clinical and epidemiological significance of infection could not be established, this study evidences that exposure of sympatric wild carnivores to parvovirus is common and geographically widespread, potentially carrying a risk to susceptible populations at the wildlife-domestic interface and to threatened species, such as the wildcat (Felis silvestris) and the critically endangered Iberian lynx (Lynx pardinus).

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Isolation of canine parvovirus from a cat manifesting clinical signs of feline panleukopenia

Cited by 122 publications

References 19 publications

Molecular analysis of carnivore Protoparvovirus detected in white blood cells of naturally infected cats

Molecular analysis of carnivore Protoparvovirus detected in white blood cells of naturally infected cats

Genetic characterization of Carnivore Parvoviruses in Spanish wildlife reveals domestic dog and cat‐related sequences

Snapshot of Viral Infections in Wild Carnivores Reveals Ubiquity of Parvovirus and Susceptibility of Egyptian Mongoose to Feline Panleukopenia Virus

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