Isolation of Cellular Fatty Acid Distributed in Unknown Rhizobial Strains

Abstract

Fatty acid (UFA) biosynthesis is essential for the maintenance of membrane structure and function in many groups of anaerobic bacteria. In this study isolation of cellular fatty acid distributed in unknown rhizobial strains collected from five different soil samples by pour plate method using YEMA medium. The five soil sample are namely Klt 1, Alg 2,Bdl 3, Rf 4 and Mdk 5.The isolated rhizobial sample was identified based on morphological and biochemical characterization. Then the purified culture was mass multiplied and used for isolation of fatty acid. The fatty acid profile of five different rhizobial isolates were identified by GC and the result of fatty acid was totally seven fatty acids from Rhizobial isolates. Among them the Myrestic acid (C:14) were present all the isolates respectively.