Isolation of Cellulolytic Bacteria from Mangrove Sediment in Dumai Marine Station Riau and the Antibacterial Activity against Pathogens

Abstract

Cellulolytic bacteria are a group of bacteria that have an ability to degrade material containing cellulose and have potency to inhibit the growth of pathogenic bacteria. The bacteria can be found in soil or sediment in the mangrove ecosystem. This research aimed to: 1) isolate cellulolylitic bacteria from mangrove sediments in Dumai Marine Station, of Riau, Indonesia, 2) to examine the antagonism against pathogenic bacteria (Escherichia coli, Pesudomonas aeruginosa, Vibrio alginolyticus), and 3) to identify and the phenotype and genetic characters of the potential isolates. The bacteria were isolated on Zobell Marine Agar 2216 added with carboxymethyl cellulose (CMC). Cellulolytic index value was obtained by reducing clear zone diameter with the bacterial colony diameter. Disc diffusion agar method was used to examine antagonism of selected isolates against pathogens. The isolates were observed for the colony and cell morphology, biochemical and genetically characters. Twenty four isolates showed cellulolytic activity and index values ranged from 1.00 to 2.86 and 0.01 to 2.12, respectively. Nine of selected isolates performed ranges of zone inhibition against E. coli, P. aeruginosa and V. alginolyticus from 2.38 ± 0.21 mm to 3.58 ± 0.83 mm, 2.75 ± 0. 59 mm - 4.81 ± 0.57 mm and 2.28 ± 0.45 mm - 4.68 ± 1.40 mm, respectively. Based on the 16S rDNA sequence analysis, three cellulolytic isolates indicated similarity to Bacillus toyonensis (99.53 %).

Potential of Single Cell Protein Production Using Waste as Growth Medium

“…In aquaculture, the addition of probiotic bacteria can increase the immunity of cultured fish because it is able to change the variation of the bacterial community in the waters [12]. In living things, cells need proteins to construct these cells [13]. .…”

“…Currently, the search for protein sources of feed containing high protein continues to be developed in the form of finished products that can be marketed. One of the efforts made is the use of B. cereus as a source of protein in feed [2]. In nature, the presence of B. cereus is very abundant so that the use of B. cereus as an alternative feed in aquaculture is very appropriate and a promising innovative solution [3].…”

OPTIMIZATION OF THE GROWTH OF PROTEOLYTIC BACTERIA Bacillus toyonensis AT DIFFERENT SALINITY AND CONCENTRATION OF TOFU WASTEWATER

“…Bacillus toyonensis merupakan salah satu genus Bacillus yang telah dijumpai di berbagai lingkungan seperti dari zona neritik Laut Arabian [5], sedimen laut di Eastern Cape, Afrika Selatan [6], dan pada sedimen laut dalam di Terusan Okinawa Selatan [7]. Jenis bakteri ini juga telah 177 e-issn: 2716-4608 p-issn: 2655-366X dijumpai pada sedimen mangrove di Stasiun Kelautan Dumai, Riau [8][9], namun belum diketahui kondisi optimal pertumbuhannya.…”

“…Isolat bakteri yang digunakan yaitu B. toyonensis strain B.S. SP 1.7 yang diperoleh dari hasil penelitian sebelumnya [8]. Sebagai kontrol bakteri ditumbuhkan pada medium TSB pada salinitas 0‰.…”

Contribution of Aerobic Cellulolytic Gut Bacteria to Cellulose Digestion in Fifteen Coastal Grapsoid Crabs Underpins Potential for Mineralization of Mangrove Production

“…In the last decade, new cellulolytic bacteria from various sources such as the gut of various organisms [ 16 ], soil [ 17 ], and plants [ 18 ] have continually been reported. Most of these studies focus on the benefit of cleaner energy sources from cellulolytic bacteria, while few investigated their ecological roles.…”