Isolation of chlorophylls and carotenoids from freshwater algae using different extraction methods

Fabrowska, Joanna; Messyasz, Beata; Szyling, Jakub; Walkowiak, Jędrzej; Łęska, Bogusława

doi:10.1111/pre.12191

Cited by 64 publications

(28 citation statements)

References 29 publications

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“…In other studies, MAE and UAE methodologies were shown to be the more efficient techniques for the chlorophyll and carotenoid isolation from freshwater green algae: C. glomerata , C. rivularis and U. flexuosa compared to the Soxhlet extraction and solid phase extraction [ 191 ].…”

Section: Novel Liquid–liquid and Solid–liquid Extraction Technologmentioning

confidence: 99%

Macroalgae as a Source of Valuable Antimicrobial Compounds: Extraction and Applications

et al. 2020

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In the last few decades, attention on new natural antimicrobial compounds has arisen due to a change in consumer preferences and the increase in the number of resistant microorganisms. Macroalgae play a special role in the pursuit of new active molecules as they have been traditionally consumed and are known for their chemical and nutritional composition and their biological properties, including antimicrobial activity. Among the bioactive molecules of algae, proteins and peptides, polysaccharides, polyphenols, polyunsaturated fatty acids and pigments can be highlighted. However, for the complete obtaining and incorporation of these molecules, it is essential to achieve easy, profitable and sustainable recovery of these compounds. For this purpose, novel liquid–liquid and solid–liquid extraction techniques have been studied, such as supercritical, ultrasound, microwave, enzymatic, high pressure, accelerated solvent and intensity pulsed electric fields extraction techniques. Moreover, different applications have been proposed for these compounds, such as preservatives in the food or cosmetic industries, as antibiotics in the pharmaceutical industry, as antibiofilm, antifouling, coating in active packaging, prebiotics or in nanoparticles. This review presents the main antimicrobial potential of macroalgae, their specific bioactive compounds and novel green extraction technologies to efficiently extract them, with emphasis on the antibacterial and antifungal data and their applications.

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Section: Novel Liquid–liquid and Solid–liquid Extraction Technologmentioning

confidence: 99%

Macroalgae as a Source of Valuable Antimicrobial Compounds: Extraction and Applications

et al. 2020

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“…Each extract was dissolved with acetone p.a at 100 ppm and measured at a wavelength of 645 nm, 662 nm and 470 nm [26]. Most carotenoids absorb lights in the region between 400 and 500 nm, while chlorophylls absorb light at 645 and 662 nm at room temperature [27].…”

Section: Assessment Of Chlorophyll a And Carotenoids Compoundsmentioning

confidence: 99%

Comparative Study on Antioxidant Activities, Total Phenolic Compound and Pigment Contents of Tropical Spirulina platensis, Gracilaria arcuata and Ulva lactuca Extracted in Different Solvents Polarity

et al. 2020

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These study aimed to determine antioxidant activity, total phenolic compound and pigment content of tropical S. platensis, G. arcuata and U. lactuca. S. platensis was extracted with aquadest (SPA), while G. arcuata and U. lactuca were extracted with n-hexane (GAH and ULH), ethyl-acetate (GAE and ULE) and methanol (GAM and ULM). The IC50 value was used to calculate the ability of extract to inhibit free radicals using DPPH (515 nm). The TPC was determined using Folin-Ciocalteu (725 nm). Phycocyanin (620 nm), phycoerythrin (652 nm), allophycocyanin (562 nm), carotenoid (470 nm) and chlorophyll (645 nm and 662 nm) were measured based on color intensity. The results indicated that SPA was able to inhibit free radicals (46.12±2.03%), contains TPC (26.64±0.16 mg/GAE samples) and dominated by phycocyanin pigments (0.301±0.09 mg/g). G. arcuata extracted with ethyl-acetate (GAE) showed the best value with IC50 136.267±0.28 ppm, TPC 303.507±1.155 mg/GAE samples, carotenoid 0.528 ±0.009 μmol/g and chlorophyll a 2.845 ±0.069 mg/g. The U. lactuca extracted with ethyl-acetate also showed the best value with IC50 462.560±2.44 ppm, TPC 210.129±1.387 mg/GAE samples, carotenoid 0.442 ±0.046 μmol/g and chlorophyll a 9.216 ±0.103 mg/g. This results conlcuded that macroalgae extracted with ethyl acetate had better activity which categorized as moderate antioxidant.

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“…Using sonication in water (and subsequent ammonium sulphate-induced protein precipitation) provided the greatest protein yields, but the protein and fatty acids content was higher with the alkaline protein solubilization [87]. An optimized ultrasound-assisted extraction of crude Ulva sulphated polysaccharides showed radical scavenging, reducing power and a macrophage-stimulating capacity [100].…”

Section: Emerging Technologiesmentioning

confidence: 99%

Ulva lactuca, A Source of Troubles and Potential Riches

2019

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Ulva lactuca is a green macro alga involved in devastating green tides observed worldwide. These green tides or blooms are a consequence of human activities. Ulva blooms occur mainly in shallow waters and the decomposition of this alga can produce dangerous vapors. Ulva lactuca is a species usually resembling lettuce, but genetic analyses demonstrated that other green algae with tubular phenotypes were U. lactuca clades although previously described as different species or even genera. The capacity for U. lactuca to adopt different phenotypes can be due to environment parameters, such as the degree of water salinity or symbiosis with bacteria. No efficient ways have been discovered to control these green tides, but the Mediterranean seas appear to be protected from blooms, which disappear rapidly in springtime. Ulva contains commercially valuable components, such as bioactive compounds, food or biofuel. The biomass due to this alga collected on beaches every year is beginning to be valorized to produce valuable compounds. This review describes different processes and strategies developed to extract these different valuable components.

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Isolation of chlorophylls and carotenoids from freshwater algae using different extraction methods

Cited by 64 publications

References 29 publications

Macroalgae as a Source of Valuable Antimicrobial Compounds: Extraction and Applications

Macroalgae as a Source of Valuable Antimicrobial Compounds: Extraction and Applications

Comparative Study on Antioxidant Activities, Total Phenolic Compound and Pigment Contents of Tropical Spirulina platensis, Gracilaria arcuata and Ulva lactuca Extracted in Different Solvents Polarity

Ulva lactuca, A Source of Troubles and Potential Riches

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