Isolation of collagen from fish waste material — skin, bone and fins

Nagai, Takeshi

doi:10.1016/s0308-8146(99)00188-0

Cited by 497 publications

(317 citation statements)

References 8 publications

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“…The presence of collagen in the fish scales, which are considered waste, was also supported by the findings of Zhang et al (2007), who reported a high amount of collagen in fish scales and bones. These results that identify collagen from a waste source, such as fish processing byproducts, have also been reported by Nagai and Suzuki (1999), who revealed that type I collagen could be isolated from fish skin, bones, and fins. We observed the most protein content was in the skin, whereas the scales and fins displayed relatively low amounts of protein.…”

Section: Resultssupporting

confidence: 81%

“…The collagens were isolated using the method described by Nagai and Suzuki (1999) with modifications. All of the samples were prepared at ambient temperature (22-23°C) with the exception of centrifugation, which was performed at a temperature not higher than 4°C.…”

Section: Isolation Of Skin Fin and Scale Collagensmentioning

confidence: 99%

“…ASC was extracted as per the method of Nagai and Suzuki (1999). All processes were carried out at the 4°C with continuous stirring.…”

Section: Extraction Of Acid Soluble Collagen (Asc)mentioning

confidence: 99%

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Isolation and characterization of collagen from fish waste material- skin, scales and fins of Catla catla and Cirrhinus mrigala

Mahboob

2014

J Food Sci Technol

122

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The collagen of skin, scales and fins of Catla catla and Cirrhinus mrigala were isolated and characterised. Nine fishes of each fish species of three weight groups were collected from a commercial fish farm. Collagen characterisation using SDS-PAGE revealed the molecular weights (kDa) of the C. catla skin, scales, and fins which ranged from 120 to 210, 70 to 201, and 68 to 137 kDa, respectively. The size of the collagen of C. mrigala skin, scales and fins ranged from 114 to 201, 77 to 210, and 70 to 147 kDa, respectively. Glycine and alanine were the most abundant amino acid, whereas tryptophan was totally absent in all selected tissues. Thus, significant variation exists in type of collagen and amino acid profile within the weight groups of the two fish species. The imino acid (proline and hydroxyproline) contents estimated in C. catla and C. mrigala skin (161-165 and 160-168), scales (155-159 and 152-161) and fins (162-171 and (152-155) residues/1,000 residues, respectively. The proximate analysis was also performed for skin, scales and fins. The maximum protein content of the skin was determined as 26.10 % and 22.90 % in the C. catla and C. mrigala, respectively, from the W 3 weight group. The scales of the W 3 weight group exhibited maximum protein contents of 25.90 and 21.77 % for C. catla and C. mrigala, respectively. The maximum protein contents (19.04 % and 18.12 %) were recorded for C. catla and C. mrigala, respectively in the fins.

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Section: Resultssupporting

confidence: 81%

Section: Isolation Of Skin Fin and Scale Collagensmentioning

confidence: 99%

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Isolation and characterization of collagen from fish waste material- skin, scales and fins of Catla catla and Cirrhinus mrigala

Mahboob

2014

J Food Sci Technol

122

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“…Search for new gelling agents to replace mammalian gelatin led to patents for fish gelatin production (Grossman and Bergman 1992;Holzer 1996) as well as several published methods for fish gelatin production (Gomez-Guillen and Montero 2001; Gudmundsson and Hafsteinsson 1997;Nagai and Suzuki 2000;Arnesen and Gildberg 2002). The commercial interest in fish gelatin has this far, however, been relatively low.…”

Section: Introductionmentioning

confidence: 99%

A comparative study on the physical, chemical and functional properties of carp skin and mammalian gelatins

2012

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Carp species forms the bulk of the aquaculture production in India and studies have shown that the filleting waste of these species, particularly skin can be a good source of gelatin. This study is a comparison of the gelatins from these unexploited sources with that of mammalian gelatins to get a better understanding of their physicochemical and functional properties with respect to mammalian gelatins. The study showed that mammalian gelatins had significantly superior physical properties viz., higher viscosity, melting & setting temperature and faster setting time. The odour scores were significantly higher (P<0.05) for bovine and porcine skin gelatins (3.1-3.12), indicating that they had a distinguishable odour and hence can be considered as inferior to fish skin gelatins in organoleptic qualities. The gel strengths of rohu and common carp skin gelatins were significantly lower than mammalian gelatins. Among the carp skin gelatins, grass carp skin gelatin was found to have better compatibility with gelatin from bovine and porcine skins.

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“…The collagen was extracted according to the method of Nagai and Suzuki (2000). All the following processes were carried out at 4°C.…”

Section: Isolation Of Collagenmentioning

confidence: 99%

Detection of collagen through FTIR and HPLC from the body and foot of Donax cuneatus Linnaeus, 1758

Nazeer¹,

Kavitha²,

Ganesh³

et al. 2011

J Food Sci Technol

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To make more effective use of available marine resources, acid soluble collagen (ASC) was isolated from body and foot of wedge clam Donax cuneatus Linnaeus, 1758 with acetic acid and was characterized for their potential and commercial applications. The yield of ASC was 17% and 23% respectively. SDS PAGE, UV and FTIR spectroscopy showed that both were type I mainly with slight differences. HPLC was used for identifying the presence of different types of amino acids, where glycine was more or less 20% in both the samples and takes the lead amino acid position and presence of imino acids (11.8 and 12.6%) has been the characteristic feature of type I collagen.

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Isolation of collagen from fish waste material — skin, bone and fins

Cited by 497 publications

References 8 publications

Isolation and characterization of collagen from fish waste material- skin, scales and fins of Catla catla and Cirrhinus mrigala

Isolation and characterization of collagen from fish waste material- skin, scales and fins of Catla catla and Cirrhinus mrigala

A comparative study on the physical, chemical and functional properties of carp skin and mammalian gelatins

Detection of collagen through FTIR and HPLC from the body and foot of Donax cuneatus Linnaeus, 1758

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