Isolation of Cryptosporidium andersoni Kawatabi type in a slaughterhouse in the northern island of Japan

Koyama, Yukari; Satoh, Masaaki; Maekawa, Kanako; Hikosaka, Kenji; Nakai, Yutaka

doi:10.1016/j.vetpar.2005.03.014

Cited by 38 publications

(25 citation statements)

References 9 publications

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“…In these reports, Cryptosporidium spp. were detected in the weaned piglets [13,21], but were rarely observed in the pigs imported to slaughterhouses or the 6-month-old pigs [13,14,23]. The present results were similar to those of previous reports.…”

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confidence: 92%

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Coprological Survey of Parasitic Infections in Pigs and Cattle in Slaughterhouse in Osaka, Japan

Matsubayashi

Kita²,

Narushima³

et al. 2009

The Journal of Veterinary Medical Science

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ABSTRACT. A coprological survey was performed at a slaughterhouse in Osaka, Japan, from 2004 to 2007 on 129 pigs reared in 8 prefectures, and on 213 cattle reared in 21 prefectures. Eimeria spp., Trichuris suis, Ascaris suum and Metastrongylus spp. infections were found in 52 (40.3%), 32 (24.8%), 19 (14.7%) and 3 pigs (2.3%), respectively, while Eimeria spp., Capillaria bovis and Trichuris sp. infections were detected in 163 (76.5%), 15 (7.0%) and 8 cattle (3.8%), respectively. Our results suggest that environmentally resistant oocysts and eggs of parasites could be widespread at the farms examined.

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supporting

confidence: 92%

“…At present, surveys on Cryptosporidium infections in pigs in Japan have been reported [13,14,21,23], but not on Giardia spp. In these reports, Cryptosporidium spp.…”

mentioning

confidence: 99%

Coprological Survey of Parasitic Infections in Pigs and Cattle in Slaughterhouse in Osaka, Japan

Matsubayashi

Kita²,

Narushima³

et al. 2009

The Journal of Veterinary Medical Science

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“…andersoni is also a major species detected in cattle, especially post-weaned or adults [24]. There are several studies on C. andersoni detected in cattle in Japan [2,11,14,16,17,20,26]. Here, we did not detect this species, perhaps because we only sampled from calves.…”

Section: Discussionmentioning

confidence: 63%

Molecular Characterization of <i>Cryptosporidium</i> Isolates from Calves in Ishikari District, Hokkaido, Japan

et al. 2013

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ABSTRACT. Cattle are major hosts of Cryptosporidium. Cryptosporidiosis in neonatal calves is associated with retarded growth, weight loss and calf mortality, and zoonotic infections in humans. Fecal samples were collected from calves in Ishikari District, Hokkaido, Japan and examined by PCR and sequence analyses. Among the 107 fecal samples collected in May and June 2012, 25 (23%) were positive for Cryptosporidium, including 8 samples (7%) having C. parvum, 10 (9%) having C. bovis and 7 (7%) having C. ryanae. This is first time C. ryanae has been detected in Hokkaido. Furthermore, it is the first detection of C. ryanae from pre-weaned calves in Japan. Microscopic observation with the flotation method is powerful and traditional tool for screening for Cryptosporidium species, but it sometimes leads to low detection of Cryptosporidium with low oocyst shedding intensity. If calves with or without diarrhea are examined using the molecular diagnostic tools, C. bovis and C. ryanae might be detected in other areas of Japan including Hokkaido. Here, the zoonotic species, C. parvum, was also observed. Therefore, calves can be potential sources of cryptosporidial infections for humans and other animals. The detection of C. parvum was statistically correlated with diarrhea in calves.

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“…Oocysts similar to those obtained from cattle in the US were not transmissible to neonatal or adult BALB/c mice and SCID mice (Koudela et al 1998). However, several successful transmissions of bovine-derived large-type oocysts, referred to as C. andersoni, to SCID mice have been reported in Japan (Kaneta and Nakai 1998;Satoh et al 2003;Matsubayashi et al 2004a;Koyama et al 2005). In the present study, we also confirmed the ability of the C. andersoni Kawatabi strain to infect SCID mice.…”

Section: Discussionmentioning

confidence: 99%

“…Wild mammals, particularly rodents, have been identified as reservoirs of Cryptosporidium (Chalmers et al 1997;Morgan et al 1999). In Japan, the presence of Cryptosporidium has been extensively studied in domestic animals raised on farms and slaughter houses (Kaneta and Nakai 1998;Koyama et al 2005); however, only a few wild animals have been investigated (Iseki 1998). We studied the presence of Cryptosporidium oocysts in wild animals and zoo housed animals in Japan and detected oocysts in large Japanese field mice, Apodemus speciosus, which were captured on the beef farm where the C. andersoni Kawatabi strain was isolated (Satoh et al 2003, Nakai et al 2004.…”

Section: Introductionmentioning

confidence: 99%

A novel genotype of Cryptosporidium muris from large Japanese field mice, Apodemus speciosus

Hikosaka

Nakai

2005

Parasitol Res

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Cryptosporidium muris-like oocysts were isolated from large Japanese field mice, Apodemus speciosus. Morphologically, these oocysts resembled those obtained from a C. andersoni Kawatabi isolate but were smaller in size than those from a C.muris isolate. Following oral inoculation of the oocysts into large Japanese field mice and SCID mice, developing stages were found in the stomach epithelium. The infectivity of the isolate to wild and laboratory mice was slightly different from that of C.muris. DNA sequences of the 18S ribosomal RNA (rRNA) gene of the isolate were not identical to those of any known Cryptosporidium spp.; however, phylogenetic analysis indicated that the isolate was a member of the C.muris cluster. Differences between the isolate and C. muris are not significant at this point; therefore, we propose that this isolate is a novel genotype of C.muris and denote it as C. muris Japanese field mouse genotype.

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Isolation of Cryptosporidium andersoni Kawatabi type in a slaughterhouse in the northern island of Japan

Cited by 38 publications

References 9 publications

Coprological Survey of Parasitic Infections in Pigs and Cattle in Slaughterhouse in Osaka, Japan

Coprological Survey of Parasitic Infections in Pigs and Cattle in Slaughterhouse in Osaka, Japan

Molecular Characterization of <i>Cryptosporidium</i> Isolates from Calves in Ishikari District, Hokkaido, Japan

A novel genotype of Cryptosporidium muris from large Japanese field mice, Apodemus speciosus

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