Isolation of Cytochrome C for Proteomics with Lindqvist-type Polyiodate Modified Metal Organic Framework

Separation of the heme protein cytochrome C using a 3D structured graphene oxide bionanocomposite as an adsorbent

“…29 The isoelectric point of Cyto C is between pH = 9.5-11.2. [1][2][3]30 Thus, when the pH is below the Cyto C isoelectric point, the protein is positively charged, and when the pH is above the Cyto C isoelectric point, the protein is negatively charged. Moreover, the GO-Agar composite has an isoelectric point below pH = 2.0, which means that when the solution pH is above 2.0, the adsorbent is negatively charged.…”

“…2 Adsorption processes using nanomaterials or bionanocomposites as adsorbents provide efficient approaches for isolating and purifying proteins. 3 The comprehension of complex interactions between the surface of biological macromolecules and nanomaterials underlies the potential biomedical applications of novel nanomaterials and nanocomposites. 4 Several applications in biotechnology rely on protein adsorption, and interactions of proteins with nanomaterials can be exploited for tissue engineering, regenerative medicine, drug delivery, and biosensors development primarily due to their mechanical properties, versatility in functionalization, and high specific surface area.…”

“…Furthermore, after Cyto C removal, some low-abundance proteins that can hardly be detected in the presence of Cyto C can be easily identified. 3 On the other hand, three-dimensional (3D) porous graphenebased composites have attracted attention for applications in adsorption processes because of their functionalities. The surface of the nanosheets in 3D graphene structures could be exploited, offering the nanocomposites with increased adsorption potential.…”

“…Furthermore, after Cyto C removal, some low-abundance proteins that can hardly be detected in the presence of Cyto C can be easily identified. 3…”