Isolation of dengue serotype 3 virus from the cerebrospinal fluid of an encephalitis patient in Hai Phong, Vietnam in 2013

Ly, Minh Huong Phu; Takamatsu, Yuki; Nabeshima, Takeshi; Hoai, Linh Ly Pham; Thi, Hang Pham; Thi, Dinh Dang; Nguyen, Ngoc Linh; Thi, Thu Thuy Nguyen; Thi, Quynh Mai Le; Buerano, Corazon C.; Morita, Kouichi; Hasebe, Futoshi

doi:10.1016/j.jcv.2015.07.295

Cited by 8 publications

(9 citation statements)

References 19 publications

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“…Outside of Thailand, DENV-3 genotype III also has been disseminated to other countries in Asia. DENV-3 genotype III was isolated as a minor genotype in Laos in 2012–2013 [ 76 ], and was first observed in Vietnam in 2013 [ 77 ], in Singapore in 2013 [ 78 ], and in Malaysia in 2008–2010 [ 74 ]. Our study revealed that the DENV-3 genotype III currently circulating in Thailand clusters with lineage-C viruses from South Asia (India and Pakistan) and from other areas in Southeast Asia (Singapore, Laos, and Viet Nam) where the virus had been detected; this cluster was independent of the lineage-A viruses (recovered from Sri Lanka, Malaysia, and Singapore) and the lineage-B viruses obtained in the Americas.…”

Section: Discussionmentioning

confidence: 99%

Emergence of genotype Cosmopolitan of dengue virus type 2 and genotype III of dengue virus type 3 in Thailand

et al. 2018

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Dengue is a mosquito-borne disease that has spread to over 100 countries. Dengue fever is caused by dengue virus (DENV), which belongs to the Flavivirus genus of the family Flaviviridae. DENV comprises 4 serotypes (DENV-1 to DENV-4), and each serotype is divided into distinct genotypes. Thailand is an endemic area where all 4 serotypes of DENV co-circulate. To understand the current genotype distribution of DENVs in Thailand, we enrolled 100 cases of fever with dengue-like symptoms at the Bamrasnaradura Infectious Diseases Institute during 2016–2017. Among them, 37 cases were shown to be dengue-positive by real-time PCR. We were able to isolate DENVs from 21 cases, including 1 DENV-1, 8 DENV-2, 4 DENV-3, and 8 DENV-4. To investigate the divergence of the viruses, RNA was extracted from isolated DENVs and viral near-whole genome sequences were determined. Phylogenetic analysis of the obtained viral sequences revealed that DENV-2 genotype Cosmopolitan was co-circulating with DENV-2 genotype Asian-I, the previously predominating genotype in Thailand. Furthermore, DENV-3 genotype III was found instead of DENV-3 genotype II. The DENV-2 Cosmopolitan and DENV-3 genotype III found in Thailand were closely related to the respective strains found in nearby countries. These results indicated that DENVs in Thailand have increased in genotypic diversity, and suggested that the DENV genotypic shift observed in other Asian countries also might be taking place in Thailand.

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Section: Discussionmentioning

confidence: 99%

Emergence of genotype Cosmopolitan of dengue virus type 2 and genotype III of dengue virus type 3 in Thailand

et al. 2018

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“…sequence for DENV-3 and two studies that reported partial E-NS1 sequences for DENV-2, have examined the genetic characteristics of neurotropic DENV. All of these DENV were isolated from CSF samples [8,[42][43][44][45]. To our knowledge, this is the first report to describe the complete genome and the molecular and virological characteristics of DENV-2 isolated from CSF samples from fatal and non-fatal dengue case-patients with neurological manifestations.…”

Section: Plos Onementioning

confidence: 93%

“…To our knowledge, this is the first report to describe the complete genome and the molecular and virological characteristics of DENV-2 isolated from CSF samples from fatal and non-fatal dengue case-patients with neurological manifestations. Compared with other dengue serotypes, DENV-2 and DENV-3 appear to be more frequently associated with encephalitis and other neurological manifestations, regardless of whether it is primary or secondary infections [43,45]. In a meta-analysis examining the severity of dengue during primary or secondary infection with different DENV serotypes, secondary infections with DENV-2 were reported to be associated with severe cases [46].…”

Section: Plos Onementioning

confidence: 99%

Complete genome analysis and characterization of neurotropic dengue virus 2 cosmopolitan genotype isolated from the cerebrospinal fluid of encephalitis patients

et al. 2020

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Dengue virus (DENV) infection remains a major public health concern in many parts of the world, including Southeast Asia and the Americas. Sri Lanka experienced its largest dengue outbreak in 2017. Neurological symptoms associated with DENV infection have increasingly been reported in both children and adults. Here, we characterize DENV type 2 (DENV-2) strains, which were isolated from cerebrospinal fluid (CSF) and/or serum of patients with dengue encephalitis. Acute serum and CSF samples from each patient were subjected to dengue-specific non-structural protein 1 (NS1) antigen test, IgM and IgG enzyme-linked immunosorbent assay (ELISA), virus isolation, conventional and real-time polymerase chain reaction (PCR), and next-generation sequencing (NGS). Among the 5 dengue encephalitis patients examined, 4 recovered and 1 died. DENV-2 strains were isolated from serum and/or CSF samples of 3 patients. The highest viral genome levels were detected in the CSF and serum of the patient who succumbed to the illness. A phylogenetic tree revealed that the DENV-2 isolates belonged to a new clade of cosmopolitan genotype and were genetically close to strains identified in China, South Korea, Singapore, Malaysia, Thailand, and the Philippines. According to the NGS analysis, greater frequencies of nonsynonymous and synonymous mutations per gene were identified in the nonstructural genes. The full genomes of serum-and CSF-derived DENV-2 from the same patient shared 99.7% similarity, indicating that the virus spread across the blood-brain barrier. This is the first report to describe neurotropic DENV-2 using whole-genome analysis and to provide the clinical, immunological, and virological characteristics of dengue encephalitis patients during a severe dengue outbreak in Sri Lanka in 2017.

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“…Although the early diagnosis of dengue fever is usually based on detection of viremia, there are several reports of DENV in cerebrospinal fluid [6], saliva and urine [7]. We are aware of only one case report of the isolation of dengue virus from a respiratory specimen [8].…”

Section: Introductionmentioning

confidence: 99%

Isolation of dengue virus from the upper respiratory tract of four patients with dengue fever

Cheng

Chen

et al. 2017

PLoS Negl Trop Dis

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BackgroundDengue fever is an important arboviral disease. The clinical manifestations vary from a mild non-specific febrile syndrome to severe life-threatening illness. The virus can usually be detected in the blood during the early stages of the disease. Dengue virus has also been found in isolated cases in the cerebrospinal fluid, urine, nasopharyngeal sections and saliva. In this report, we describe the isolation of dengue virus from the upper respiratory tract of four confirmed cases of dengue.MethodsWe reviewed all laboratory reports of the isolation of dengue virus from respiratory specimens at the clinical microbiology laboratory of the Kaohsiung Veterans General Hospital during 2007 to 2015. We then examined the medical records of the cases from whom the virus was isolated to determine their demographic characteristics, family contacts, clinical signs and symptoms, course of illness and laboratory findings.ResultsDengue virus was identified in four patients from a nasopharyngeal or throat culture. Two were classified as group A dengue (dengue without warning signs), one as group B (dengue with warning signs) and one as group C (severe dengue). All had respiratory symptoms. Half had family members with similar respiratory symptoms during the period of their illnesses. All of the patients recovered uneventfully.ConclusionsThe isolation of dengue virus from respiratory specimens of patients with cough, rhinorrhea and nasal congestion, although rare, raises the possibility that the virus is capable of transmission by the aerosol route among close contacts. This concept is supported by studies that show that the virus can replicate in cultures of respiratory epithelium and can be transmitted through mucocutaneous exposure to blood from infected patients. However, current evidence is insufficient to prove the hypothesis of transmission through the respiratory route. Further studies will be needed to determine the frequency of respiratory colonization, viable virus titers in respiratory secretions and molecular genetic evidence of transmission among close contacts.

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Isolation of dengue serotype 3 virus from the cerebrospinal fluid of an encephalitis patient in Hai Phong, Vietnam in 2013

Cited by 8 publications

References 19 publications

Emergence of genotype Cosmopolitan of dengue virus type 2 and genotype III of dengue virus type 3 in Thailand

Emergence of genotype Cosmopolitan of dengue virus type 2 and genotype III of dengue virus type 3 in Thailand

Complete genome analysis and characterization of neurotropic dengue virus 2 cosmopolitan genotype isolated from the cerebrospinal fluid of encephalitis patients

Isolation of dengue virus from the upper respiratory tract of four patients with dengue fever

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