Isolation of Dental Pulp Stem Cells and their In Vitro Differentiation into Odontoblast-like Cells

Ahmed, Nermeen; Aboul-Ezz, Eman H.; Zakhary, Siza; El-Badry, Tarek H.; Ramzy, Magda

doi:10.3889/mjms.1857-5773.2011.0142

Cited by 5 publications

(3 citation statements)

References 33 publications

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“…These cells were successfully differentiated into odontoblast-like cells by odontogenic-induction medium, and this differentiation was confirmed by the positive mineralisation capability. Moreover, these cells successfully adipogenic differentiated and that was confirmed by the oil red O stain [ 13 , 14 , 10 ].…”

Section: Discussionmentioning

confidence: 99%

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The Effect of Three Different Biomaterials on Proliferation and Viability of Human Dental Pulp Stem Cells (In-vitro Study)

Mohamed¹,

Abdelfattah²,

Aboul-Ezz³

2017

Open Access Maced J Med Sci

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BACKGROUND:Biomaterial cytotoxicity on dental stem cells plays a critical role in managing the regeneration of dental tissue.AIM:The aim of the present study was to evaluate the effect of Nano-hydroxy apatite (NHA), Mineral trioxide aggregate (MTA), and Calcium-enriched mixture (CEM) on the proliferation, and viability of human dental pulp stem cells (hDPSCs) isolated from third molar teeth.METHODS:Cultured DPSCs were characterized and the tested biomaterials were shaped into cylinders then inserted directly on the DPSCs. Proliferation and viability percentage of DPSCs were evaluated at 1, 3, 5, 7, 9, 11, and 14 days of culture.RESULTS:The biomaterials supplemented DPSCs showed a significant initial decrease in cell count and viability percentage at day one. Then, a rise in cell counts and viabilities was noticed after that. There was a decrease in cell counts, and viabilities in the NHA supplemented cells in comparison to other tested biomaterials.CONCLUSIONS:All tested biomaterials maintain the proliferation of DPSCs for different durations. NHA showed less proliferative and more cytotoxic effect than other tested materials.

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Section: Discussionmentioning

confidence: 99%

“…All primers used in the present study were selected and designed according to Ahmed et al . [ 10 ] ( Table 1 ) then synthesised by Invitrogen. PCR amplification experiment was triplicated, and then the PCR products were analysed.…”

Section: Methodsmentioning

confidence: 99%

The Effect of Three Different Biomaterials on Proliferation and Viability of Human Dental Pulp Stem Cells (In-vitro Study)

Mohamed¹,

Abdelfattah²,

Aboul-Ezz³

2017

Open Access Maced J Med Sci

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“…Regenerative endodontics may offer an alternative method to save teeth that may have compromised structural integrity. Recently, the role of stem cells for hard tissue formation has considerably increased attention of researchers as these cells can be a possible, fascinating source of stable differentiated cells, capable of inducing hard tissue formation (16) .…”

Section: Discussionmentioning

confidence: 99%

Effect of Bioactive Glass Nano Particulates and Nano Hydroxyapatite Coated by Chitosan on Odontogenic Differentiation and Proliferation of human Dental Pulp Stem Cells

Ali

Mahran

Yehia

2022

Egyptian Dental Journal

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This study aimed to evaluate effect of Nano hydroxyapatite coated by Chitosan (NHAP/CS) and bioactive glass nano particulates (nBG) on odontogenic differentiation and proliferation of human dental pulp stem cells (hDPSCs).Methods: hDPSCs were seeded in direct contact with the corresponding Nano-biomaterials in 5 groups: Group (Ι): NHAP (10µg/ml), Group (ΙΙ): NHAP/CS (10µg/ml), Group (ΙΙΙ): nBG (500µg/ml). Group (IV): hDPSCs were cultured with osteogenic medium as a positive control while Group (V): hDPSCs were cultured with Dulbecco's Modified Eagle Medium (DMEM) only as a negative control. Odontogenic differentiation was evaluated based on mineralization related genes .Gene expressions were verified using alkaline phosphatase (ALP) assay and immunofluorescence staining of Dentin matrix protein (DMP-1). Proliferation of cells was evaluated via Trypan Blue staining test and methylthiazol tetrazolium (MTT) assay.Results: All the tested Nano biomaterials exhibited favorable media for odontogenic differentiation and proliferation of hDPSCs. NHAP/CS showed the highest proliferation index via MTT, highest ALP concentration and highest DMP-1Immunofluorescence staining.Conclusions: Compared to pure NHAP and a major biomaterial as nBG, NHAP/CS can induce odontogenic differentiation and proliferation of hDPSCs and could be considered as a good candidate for dentin-pulp regenerative procedures.

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A systematic review of postural control during single-leg stance in patients with untreated anterior cruciate ligament injury

Negahban

Mazaheri

Kingma

et al. 2013

Knee Surg Sports Traumatol Arthrosc

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The present review indicates that postural control is impaired in both legs, especially injured leg. The result of within-group difference in eyes open condition confirms bilateral deficit of postural control. However, the within-group difference during eyes closed condition indicates again that ACL injury affects the injured leg more than the uninjured leg. In designing rehabilitation protocols, clinicians should consider training postural control of not just the injured but also the uninjured leg.

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Isolation of Dental Pulp Stem Cells and their In Vitro Differentiation into Odontoblast-like Cells

Cited by 5 publications

References 33 publications

The Effect of Three Different Biomaterials on Proliferation and Viability of Human Dental Pulp Stem Cells (In-vitro Study)

The Effect of Three Different Biomaterials on Proliferation and Viability of Human Dental Pulp Stem Cells (In-vitro Study)

Effect of Bioactive Glass Nano Particulates and Nano Hydroxyapatite Coated by Chitosan on Odontogenic Differentiation and Proliferation of human Dental Pulp Stem Cells

A systematic review of postural control during single-leg stance in patients with untreated anterior cruciate ligament injury

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