Isolation of DNA and DNA polymerase from MC29 tumor virus

Weber, George H.; Deeney, A.O'c.; Beaudreau, G. S.

doi:10.1016/0005-2787(73)90392-4

Cited by 6 publications

(1 citation statement)

References 14 publications

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“…A number of reports (19,20) show that the RNA from L cell virions, is normal but the possibility of substantial amounts of DNA in these particles as in other retroviral virions (24,25) has not been examined. A number of reports (19,20) show that the RNA from L cell virions, is normal but the possibility of substantial amounts of DNA in these particles as in other retroviral virions (24,25) has not been examined.…”

Section: Discussionmentioning

confidence: 99%

Isolation and characterisation of the L cell virion DNA polymerase

Hallinan

Lee

Rozee

1981

Archives of Virology

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The DNA polymerase of the L-cell virion (LCV) was partially purified by chromatography on DEAE cellulose. This enzyme transcribed poly(A) . oligo (dT), poly(C) . oligo(dG), and poly(Cm) . oligo(dG), had a molecular weight of 77,000 daltons and reacted like other murine viral RNA directed DNA polymerases to anti reverse transcriptase specific IgG preparations indicating that it was probably a typical murine viral reverse transcriptase. In addition, like other partially purified mammalian viral reverse transcriptases the LCV DNA polymerase exhibited template independent primer stimulated DNA synthesis. The significance of these results to the unusual endogenous activity of the LCV is discussed.

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