Isolation of DNA polymerase alpha from germinated wheat embryos

Balmukhanov, T. S.; Erekenov, Abay M.; Ajtkhozhina, Nagima A.

doi:10.1007/bf00788748

Cited by 5 publications

(3 citation statements)

References 16 publications

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“…a-type DNA polymerases have been reported for different plant species (Bryant, 1980;Litvak and Castroviejo, 1987) and purified to different extents from rice (Amileni et al, 1979), cauliflower (Yamaguchi et al, 1979), wheat (Laquel et al, 1990;Balmukhanov et al, 1992), pea (Bryant et al, 1992) and maize (Coello et al, 1992).…”

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confidence: 99%

Maize DNA Polymerase 2 is a Phosphoprotein with Increasing Activity During Germination

Coello

Vázquez‐Ramos

1995

European Journal of Biochemistry

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DNA replication is a late event during maize germination and DNA polymerase activity increases as germination proceeds. A replicative a-type DNA polymerase has been purified from maize seeds (DNA polymerase 2) and has been shown to be a multisubunit complex [Coello, P., Rodriguez, R., Garcia, E. Phosphate incorporated into the different polypeptides in the 11 -14-h period remained stable for at least the next 10 h (to 24 h of germination), the period of maximal enzyme activity. However, DNA polymerase 2 processivity was very similar in freshly prepared 3-h and 24-h enzymes, and no evidence was found that polymerase activity was modified by in vitro phosphorylation. The significance of these results is discussed.

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confidence: 99%

Maize DNA Polymerase 2 is a Phosphoprotein with Increasing Activity During Germination

Coello

Vázquez‐Ramos

1995

European Journal of Biochemistry

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“…It is worth mentioning that a wheat a-type DNA polymerase described recently has subunits of molecular mass 104, 79,60, and 25 kDa (2), not very different to the mass of some of the polypeptides described in this work. The authors give no indi----cation of which could be the catalytic subunit or of any other accompanying activity.…”

Section: Discussionmentioning

confidence: 91%

“…An a-type enzyme was partially purified from pea and shown to be part of a large multiprotein complex (4). Evidence for a and 6 types was found in wheat (2,11,14). In maize seeds, a high molecular mass D N A polymerase was purified and it w a s s h o w n to be an a-type using antibodies against calf thymus D N A polymerase a.…”

Section: Introductionmentioning

confidence: 98%

Preparation of an antibody against a maize DNA polymerase holoenzyme: identification of the polymerase catalytic subunit

Coello-Coutiño¹,

García-Ramírez²,

Vázquez‐Ramos³

1994

Can. J. Bot.

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COELLO-COUTIRO, P., GARC~A-RAM~REZ, E., and ~AZQUEZ-RAMOS, J.M. 1994. Preparation of an antibody against a maize DNA polymerase holoenzyme: identification of the polymerase catalytic subunit. Can. J. Bot. 72: 818-822. Three different DNA polymerase activities can be separated from germinating maize axes through DEAE-cellulose chromatography. Of these, DNA polymerase 2 appears to be a replicative-type enzyme composed of several subunits. An antibody has been developed against the DNA polymerase 2 multisubunit complex, which mainly recognizes a polypeptide of molecular weight around 90 kDa. Polypeptides of molecular mass of 83, 70, 60, 55, 45, and 24 kDa are also recognized. Activity gels showed that the 90-kDa polypeptide possesses catalytic activity. DNA polymerases 1 and 3 are not recognized by the antibody and their activities are not reduced. However, DNA polymerase 2 activity is reduced by 70%. The nature of the different DNA polymerase accompanying subunits is discussed.

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Regulating DNA Replication in Plants

Sánchez

Costas

Sequeira-Mendes

et al. 2012

Cold Spring Harbor Perspectives in Biology

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Chromosomal DNA replication in plants has requirements and constraints similar to those in other eukaryotes. However, some aspects are plant-specific. Studies of DNA replication control in plants, which have unique developmental strategies, can offer unparalleled opportunities of comparing regulatory processes with yeast and, particularly, metazoa to identify common trends and basic rules. In addition to the comparative molecular and biochemical studies, genomic studies in plants that started with Arabidopsis thaliana in the year 2000 have now expanded to several dozens of species. This, together with the applicability of genomic approaches and the availability of a large collection of mutants, underscores the enormous potential to study DNA replication control in a whole developing organism. Recent advances in this field with particular focus on the DNA replication proteins, the nature of replication origins and their epigenetic landscape, and the control of endoreplication will be reviewed.

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Isolation of DNA polymerase alpha from germinated wheat embryos

Cited by 5 publications

References 16 publications

Maize DNA Polymerase 2 is a Phosphoprotein with Increasing Activity During Germination

Maize DNA Polymerase 2 is a Phosphoprotein with Increasing Activity During Germination

Preparation of an antibody against a maize DNA polymerase holoenzyme: identification of the polymerase catalytic subunit

Regulating DNA Replication in Plants

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