Isolation of Elemental Sulfur as a Self-Growth-Inhibiting Substance Produced by
            <i>Legionella pneumophila</i>

Inoue, Hiroaki; Kawano, Genji; Nagasawa, Hiromichi; Sakuda, Shohei

doi:10.1128/aem.68.10.4809-4811.2002

Cited by 11 publications

(5 citation statements)

References 9 publications

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“…14 At the same time, sulfur is recognized as an important element for plant growth through balanced fertilization and is sometimes synthesized naturally in its elemental form by some plants and microorganisms as protection. 11,12 Sulfur is indeed more acceptable as a safer organic pesticide for agricultural firms and also a useful nutrient for the plants. 13 Some reports have shown the potential inhibitory effect of sulfur nanoparticles on several microorganisms viz., Pseudomonas areuginosa, Styphylococcus areus, Candida albicans, Aspergillus flavus, Aspergillus niger, Fusarium oxysporum.…”

Section: Introductionmentioning

confidence: 99%

Use of sulfur nanoparticles as a green pesticide on Fusarium solani and Venturia inaequalis phytopathogens

2013

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Applications of nanoparticle based fungicides have been increasing gradually in recent years because of many advantages over conventional chemical fungicides. This study presents fungicidal efficiency of sulfur nanoparticles (SNPs) against two phytopathogens, Fusarium solani (isolated from an infected tomato leaf, responsible for early blight and Fusarium wilt diseases) and Venturia inaequalis (responsible for the apple scab disease). Different parameters, such as particle size, particle concentration, surfactant media etc., which affect the efficiency of the fungicidal effect were also studied. It has been found that small sized particles (y35 nm) are very effective in preventing the fungal growth. Microscopic study confirmed that the fungicidal effect is mainly because of the deposition of particles on the cell wall and subsequent damage of the cell wall. A plausible imbalance in the cell wall structure was also supported by a Biuret assay test. SNP based fungicide can be useful for the protection of important crops such as tomato, potato, apple, grape etc., from different diseases, mainly for ''organic'' farming.

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Section: Introductionmentioning

confidence: 99%

Use of sulfur nanoparticles as a green pesticide on Fusarium solani and Venturia inaequalis phytopathogens

2013

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“…Diaion HP-20 resin is a synthetic absorbent of cross-linked polystyrene, and spherical particles of the resin with diameters of 0.5 mm can be easily recovered by filtration from the culture broth without contamination of the bacterial cells. We recently reported that elemental sulfur was isolated from methanol extracts of Diaion HP-20 resins used to culture the bacteria as a main growth-inhibiting substance produced endogenously by L. pneumophila (Inoue et al, 2002). Since some active fractions other than that containing (60/40) fraction.…”

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confidence: 99%

Isolation of Indole-3-Aldehyde As a Growth Inhibitor of Legionella pneumophila from Diaion HP-20 Resins Used to Culture the Bacteria

Inoue¹,

Yajima

Kawano³

et al. 2004

Biocontrol Sci.

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“…To date, some biochemical information regarding such growth inhibitors is available; for example, the precise characterization of the high-cell-density culture by Nakano et al (11) revealed that acetic acid serves as a self-growth inhibitor for E. coli. Inoue et al (6) found that the growth of Legionella pneumophila was enhanced by the addition of Diaion resin to the culture, and sulfur was identified as the self-growth inhibitor of this bacterium. It is predicted that the elimination of lactic acid and ammonia is responsible for the growth enhancement of mammalian cells in dialysis culture (9,14).…”

Section: Discussionmentioning

confidence: 99%

Identification of Indole Derivatives as Self-Growth Inhibitors of Symbiobacterium thermophilum , a Unique Bacterium Whose Growth Depends on Coculture with a Bacillus sp

Watsuji

Yamada

Yamabe

et al. 2007

Appl Environ Microbiol

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Symbiobacterium thermophilum is a syntrophic bacterium whose growth depends on coculture with a Bacillus sp. Recently, we discovered that CO 2 generated by Bacillus is the major inducer for the growth of S. thermophilum; however, the evidence suggested that an additional element is required for its full growth. Here, we studied the self-growth-inhibitory substances produced by S. thermophilum. We succeeded in purifying two substances from an ether extract of the culture supernatant of S. thermophilum by multiple steps of reverse-phase chromatography. Electron ionization mass spectrometry and nuclear magnetic resonance analyses of the purified preparation identified the substances as 2,2-bis(3-indolyl)indoxyl (BII) and 1,1-bis(3-indolyl)ethane (BIE). The pure growth of S. thermophilum was inhibited by authentic BII and BIE with MICs of 12 and 7 g/ml, respectively; however, its growth in coculture with Bacillus was not inhibited by BII at the saturation concentration and was inhibited by BIE with an MIC of 14 g/ml. Both BII and BIE inhibited the growth of other microorganisms. Unexpectedly, the accumulation levels of both BII and BIE in the pure culture of S. thermophilum were far lower than the MICs (<0.1 g/ml) while a marked amount of BIE (6 to 7 g/ml) equivalent to the MIC had accumulated in the coculture. An exogenous supply of surfactin alleviated the sensitivities of several BIE-sensitive bacteria against BIE. The results suggest that Bacillus benefits S. thermophilum by detoxifying BII and BIE in the coculture. A similar mechanism may underlie mutualistic relationships between different microorganisms.Symbiobacterium thermophilum is a thermophilic bacterium that was originally isolated by its ability to produce a thermostable tryptophanase from compost collected at Hiroshima, Japan (19). This bacterium is characterized by a syntrophic property that is reproduced under a laboratory culture condition; although it does not grow or shows impaired growth with a very low cellular yield in pure culture, it effectively propagates, achieving a density of approximately 5 ϫ 10 8 cells/ml, when it is cocultured with the cognate Bacillus sp. strain S, which was isolated together with S. thermophilum (19). The molecular phylogenetic and genomic sequencing studies (13, 22) uncovered unique genetic and taxonomic features of S. thermophilum. S. thermophilum exhibits a number of genetic features associated with Firmicutes (low-GϩC, gram-positive bacteria represented by Bacillus and Clostridium), despite its high GϩC content (68.5%). This unusual property has now become a new topic in bacterial systematics (2, 4) and suggests that this kind of bacterium has been left uncharacterized due to its "unculturable nature," despite its wide distribution in the natural environment (20). We are interested in the fundamental details of the syntrophic feature of S. thermophilum and expect that this information will provide us with new knowledge regarding not only the microbial physiology but also the issue of unculturability of environme...

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Isolation of Elemental Sulfur as a Self-Growth-Inhibiting Substance Produced by Legionella pneumophila

Cited by 11 publications

References 9 publications

Use of sulfur nanoparticles as a green pesticide on Fusarium solani and Venturia inaequalis phytopathogens

Use of sulfur nanoparticles as a green pesticide on Fusarium solani and Venturia inaequalis phytopathogens

Isolation of Indole-3-Aldehyde As a Growth Inhibitor of Legionella pneumophila from Diaion HP-20 Resins Used to Culture the Bacteria

Identification of Indole Derivatives as Self-Growth Inhibitors of Symbiobacterium thermophilum , a Unique Bacterium Whose Growth Depends on Coculture with a Bacillus sp

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