Isolation of Enteric Bacteria, and Immunohistochemical Detection of the Bacterial Antigens from Milk-spotted Livers of Pigs

Relationship between Bacterial Translocation and Isolation of Enteric Bacteria from Extraintestinal Organs in Slaughtered Pigs

“…John [10] suspected that the infective Ascaris larvae carried the intestinal bacteria from the intestine to the liver in the ascariasis pigs with the severe hepatic inflammatory lesions. However, these intestinal bacteria were also isolated from the site of normal hepatic parenchyma without milk-spotted lesions, which is characteristic to an ascariasis [12,13,15]. Furthermore immunohistochemical detection of antigens using polyclonal antibodies against non-transformed E. coli strain K12 C600 (DakoCytomation, Inc., Tokyo, Japan) did not show a relationship between the distributions of positive antigens and hepatic white spot lesions [12].…”

“…Microbes, suspected to be of intestinal origin, such as Escherichia coli [12,13], Lactobacillus sp. [12], Lactobacillus casei [13], Lactobacillus salivalius [13], Propionibacterium sp.…”

“…[12], Lactobacillus casei [13], Lactobacillus salivalius [13], Propionibacterium sp. [12], Propionibacterium acnes, Propionibacterium granulosum, Propionibacterium avidum [15], Enterococcus sp. or Streptococcus sp.…”

“…and Staphylococcus sp. [12] have been isolated from livers of pigs with ascariasis. John [10] suspected that the infective Ascaris larvae carried the intestinal bacteria from the intestine to the liver in the ascariasis pigs with the severe hepatic inflammatory lesions.…”

“…However, these intestinal bacteria were also isolated from the site of normal hepatic parenchyma without milk-spotted lesions, which is characteristic to an ascariasis [12,13,15]. Furthermore immunohistochemical detection of antigens using polyclonal antibodies against non-transformed E. coli strain K12 C600 (DakoCytomation, Inc., Tokyo, Japan) did not show a relationship between the distributions of positive antigens and hepatic white spot lesions [12]. These findings have raised the possibility of other factors associated with the bacteria isolated from porcine livers affected with ascariasis.…”