Isolation of enterococci, their antimicrobial susceptibility patterns and associated factors among patients attending at the University of Gondar Teaching Hospital

Yilema, Amelework; Moges, Feleke; Tadele, Sisay; Endris, Mengistu; Kassu, Afework; Abebe, Wondwossen; Ayalew, Getnet

doi:10.1186/s12879-017-2363-3

Cited by 39 publications

(66 citation statements)

References 20 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Regarding antimicrobial susceptibility, the current study reported high resistance rates among enterococcus isolates to a wide range of antimicrobials. These findings agree with many reports from Egypt [21,23,29] and other countries [3]. This is a serious medical challenge, as it reduces the chance of treatment, prolongs the hospital stay and increases the cost of medical care.…”

Section: Discussionsupporting

confidence: 92%

“…Enterococci are normal intestinal flora of both animals and humans, however the importance of enterococci as pathogenic bacteria has increased with the development of their resistance to multiple drugs [1,2]. Two species of enterococci have been recognized as major hospital acquired pathogens (Enterococcus faecium, "E. faecium" and Enterococcus faecalis, "E. faecalis"), particularly vancomycin resistant strains (VRE) [1,3]. VRE are multi drug resistant bacteria which can spread in hospitals through medical staff, environmental surfaces and instruments causing healthcare-associated infections that are associated with increasing mortality rates and length of hospital stay [2,4].…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

First detection of vanB phenotype-vanA genotype vancomycin-resistant enterococci in Egypt

Khairy

Mahmoud

Esmail

et al. 2019

J Infect Dev Ctries

View full text Add to dashboard Cite

Introduction: Enterococci have emerged in last two decades as serious hospital acquired pathogens particularly vancomycin resistant strains (VRE). The study aimed to identify the prevalence of enterococcal isolation from hospital infections and colonization as well as determine vancomycin resistance phenotypes and genotypes. Methods: Sixty enterococcus isolates were isolated from patients, health care workers and hospital environment, identified and tested for antimicrobial susceptibility. Enterococcus species were identified by Real-time PCR and vancomycin resistance was assessed by agar dilution method and Real-time PCR. Results; out of 300 samples (20%) were enterococci (53.3% were E. faecium, 31.7% E. faecalis and 10% other enterococci). Among of them 40/60 (66, 6%) were isolated from infections and 33.3% were isolated from colonization. multiple drug resistance was reported in (100%) of isolates, while (95%) and (45%) of isolates were resistant to vancomycin and ticoplanin respectively. VanA phenotype, vanA genotype was identified in (47.4%) of isolates, while vanB phenotype, vanA genotype was identified in (33.3%) of vancomycin resistant isolates. Conclusion; VanB phenotype-vanA genotype was identified in (33.3%) of vancomycin resistant enterococcal isolates. To our knowledge it is the first identified incidence of such strains in Egypt and Africa.

show abstract

Section: Discussionsupporting

confidence: 92%

Section: Introductionmentioning

confidence: 99%

First detection of vanB phenotype-vanA genotype vancomycin-resistant enterococci in Egypt

Khairy

Mahmoud

Esmail

et al. 2019

J Infect Dev Ctries

View full text Add to dashboard Cite

show abstract

“…All of the 20 studies included in this review were crosssectional by design. Most of the studies were reported from Amhara region (n = 8) [30][31][32][33][34][35][36][37] and Addis Ababa (n = 7) [38][39][40][41][42][43][44]. The remaining studies were from Oromia (n = 4) [45][46][47][48] and Southern nations (n = 1) [23].…”

Section: Characteristics Of Included Studiesmentioning

confidence: 99%

Prevalence of Vancomycin resistant enterococci (VRE) in Ethiopia: a systematic review and meta-analysis

2020

View full text Add to dashboard Cite

Background: The emergence of Vancomycin resistant enterococci (VRE) poses a major public health problem since it was first reported. Although the rising rates of VRE infections are being reported elsewhere in the worldwide; there is limited national pooled data in Ethiopia. Therefore, this study was aimed to estimate the pooled prevalence of VRE and antimicrobial resistance profiles of enterococci in Ethiopia. Methods: Literature search was done at PubMed, EMBASE, Google scholar, African Journals online (AJOL) and Addis Ababa University repository following the Preferred Reporting Items for Systematic Reviews and Meta-Analyses (PRISMA) guideline. Both published and unpublished studies reporting the prevalence of VRE until June 30, 2019 were included. Data were extracted using Microsoft Excel and copied to Comprehensive Meta-analysis (CMA 2.0) for analysis. Pooled estimate of VRE was computed using the random effects model and the 95% CIs. The level of heterogeneity was assessed using Cochran's Q and I 2 tests. Publication bias was checked by visual inspection of funnel plots and Begg's and/or Egger's test. Results: Twenty studies fulfilled the eligibility criteria and found with relevant data. A total of 831 enterococci and 71 VRE isolates were included in the analysis. The pooled prevalence of VRE was 14.8% (95% CI; 8.7-24.3; I 2 = 74.05%; P < 0.001). Compared to vancomycin resistance, enterococci had higher rate of resistance to Penicillin (60.7%), Amoxicillin (56.5%), Doxycycline (55.1%) and Tetracycline (53.7%). Relatively low rate of resistance was found for Daptomycin and Linezolid with a pooled estimate of 3.2% (95% CI, 0.5-19.7%) and 9.9% (95% CI, 2.8-29.0%); respectively. The overall pooled multidrug resistance (MDR) rate of enterococci was 60.0% (95% CI, 42.9-75.0%). Conclusion: The prevalence of VRE and drug resistant enterococci are on the rise in Ethiopia. Enterococcal isolates showed resistance to one or more of the commonly prescribed drugs in different or the same drug lines. Multidrug resistant (MDR) enterococci were also found. Although the rates were low, the emergence of resistance to Daptomycin and Linezolid is an alarm for searching new ways for the treatment and control of VRE infections. Adherence to antimicrobial stewardship, comprehensive testing and ongoing monitoring of VRE infections in the health care settings are required.

show abstract

“…11 The prevalence of Enterococcus in wound infection in our study (38.02%) correlates with study done by Vandamme et al (37.9%) 12 and Amelework et al (25%). 13 The prevalence of Enterococcus in blood was 7.04% as compared to 4.23% and 20.8% reported in different studies. 11,13 Enterococcus faecium was found to be more drug resistant.…”

Section: Discussionmentioning

confidence: 71%

Vancomycin Resistance among Enterococci: Further Limiting Our Therapeutic Options

Ohri¹,

Singh²,

Sidhu³

et al. 2020

IJCMR

View full text Add to dashboard Cite

Introduction: Enterococci are indigenous flora of the intestinal tract, oral cavity & genitourinary tract of human. Over recent years, there is increased interest in Enterococci not only because of their serious infections but because of their increasing resistance to many antimicrobials. Vancomycin being the only alternative available. But over the time, there has been increase in Vancomycin Resistance which has spread globally. The aim of this study was to determine the prevalence of Vancomycin Resistant Enterococci (VRE) isolated from various clinical specimens in a tertiary care hospital in North India. Material and methods: A cross-sectional study was conducted in the Department of Microbiology, Government Medical College, Amritsar from July 1 st , 2018 to June 30 th , 2019. All the samples received were processed and identification of Enterococci was made by using standard microbiological techniques. Antimicrobial susceptibility was performed by Kirby Bauer disc diffusion method as per CLSI guidelines. Results: Out of total clinical samples (11,098), 3,551 (31.9%) were found to be culture positive. Among the culture positive, 91 (2.56%) isolates were identified as Enterococcus species comprising of 37 E.faecalis (41%) and 54 E.faecium (59%). Maximum number of Enterococci were isolated from urine samples (54.92%) followed by pus & body fluids (38.02%) and blood (7.04%). 9.52% of E.faecium isolates were found to be resistant to vancomycin. All the strains were 100% susceptible to Linezolid, Teicoplanin & Quinupristin-dalfopristin. Conclusion: Enterococci have become the major pathogenic bacteria that cause hospital-acquired infections due to multiple-antimicrobial resistance. VRE has emerged as important nosocomial pathogen and pose serious threat to patients. Vancomycin should be cautiously used else we would be left with very few therapeutic options.

show abstract

Isolation of enterococci, their antimicrobial susceptibility patterns and associated factors among patients attending at the University of Gondar Teaching Hospital

Cited by 39 publications

References 20 publications

First detection of vanB phenotype-vanA genotype vancomycin-resistant enterococci in Egypt

First detection of vanB phenotype-vanA genotype vancomycin-resistant enterococci in Egypt

Prevalence of Vancomycin resistant enterococci (VRE) in Ethiopia: a systematic review and meta-analysis

Vancomycin Resistance among Enterococci: Further Limiting Our Therapeutic Options

Contact Info

Product

Resources

About