Isolation of enterotoxigenic, hemolytic, and antibiotic-resistant Aeromonas hydrophila strains from infected fish in Bangladesh

Rahim, Zeaur; Sanyal, Sudip; Aziz, Kanwal; Huq, M. I.; Chowdhury, Asif Alam

doi:10.1128/aem.48.4.865-867.1984

Cited by 59 publications

(15 citation statements)

References 17 publications

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“…The emergence and worldwide distribution of drug‐resistant strains of A. hydrophila has previously been reported (Ansary, Haneef, Torres, & Yadav, ; McNicol et al, ; Rahim, Sanyal, Aziz, Huq, & Chowdhury, ; Thayumanavan, Vivekanandhan, Savithamani, Subashkumar, & Lakshmanaperumalsamy, ). One A. hydrophila strain isolated from the internal organs of diseased fish was resistant to more than 10 kinds of antibiotics (Sahoo et al, ).…”

Section: Discussionmentioning

confidence: 93%

Role of LuxR‐type regulators in fish pathogenic Aeromonas hydrophila

Mao

Qin

Kang

et al. 2019

Journal of Fish Diseases

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LuxR‐type transcriptional factors are essential in many bacterial physiological processes. However, there have been no reports on their roles in Aeromonas hydrophila. In this study, six stable silent strains were constructed using shRNA. Significant decreases in the expression levels of luxR05, luxR08, luxR19, luxR11, luxR164 and luxR165 were shown in their respective strains by qRT‐PCR. The luxR05‐RNAi and luxR164‐RNAi exhibit the most significant changes in sensitivity to kanamycin and gentamicin. The luxR05‐RNAi showed minimum biofilm formation and the least motility, while luxR164‐RNAi showed minimum biofilm formation, adhesion, growth and extracellular protease activity compared to the wild‐type strain. In summary, the results of this paper suggest that all six luxR genes are involved in multiple physiological processes in A. hydrophila and that the roles of luxR05 and luxR164 are highly significant. The sensitivity of luxR05‐RNAi and luxR164‐RNAi to drugs may be closely related to biofilm formation. The luxR05 may play an important role in the pathogenicity of A. hydrophila by regulating the movement, adhesion and biofilm formation of bacteria, whereas luxR164 may be involved in similar functions by regulating bacterial adhesion, extracellular enzyme activity and growth. These results help further our understanding of the drug resistance and pathogenesis of A. hydrophila.

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Section: Discussionmentioning

confidence: 93%

Role of LuxR‐type regulators in fish pathogenic Aeromonas hydrophila

Mao

Qin

Kang

et al. 2019

Journal of Fish Diseases

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“…As farmed fish are often treated with a wide variety of antimicrobial compounds (Austin and Austin, 1993), this has resulted in the development of resistant strains (Barnes et al, 1991;Saitanu et al, 1994). Although the majority of studies on the development of antibiotic resistant bacteria have mainly focussed on cattle, poultry and swine, the emergence of antibiotic resistant strains of fish pathogens has also been reported from temperate and tropical aquaculture systems (Rahim et al, 1984;Spanggaard et al, 1993;Kerry et al, 1995). This paper reports the results of a study on the phenotypic characteristics, hemolytic activity and antibiotic resistance of motile aeromonads encountered in the intestines of farm-raised fresh water fish such as Catla catla, Labeo rohita and Ctenopharyngodon idella.…”

Section: Introductionmentioning

confidence: 99%

Antibiotic resistance pattern of motile aeromonads from farm raised fresh water fish

Hatha

Vivekanandhan

Joice

et al. 2005

International Journal of Food Microbiology

161

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Motile aeromonads isolated from the intestines of farm-raised freshwater fish such as Catla catla, Labeo rohita and Ctenopharyngodon idella have been characterized to species level. Morphological and physiological grouping revealed 61% Aeromonas hydrophila, 30% Aeromonas caviae, 7% Aeromonas sobria and 2% which remained unidentified. Hemolytic activity was detected mostly in A. hydrophila, while only half of the A. sobria and A. caviae showed this activity. Antibiotic resistance patterns of the strains revealed that they had acquired a relatively higher resistance to oxytetracycline, amoxycillin, ampicillin, novobiocin and polymixin-B, implicating possible use of these antibiotics in the aquaculture systems.

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“…Motile Aeromonas strains have been associated with a wide range of infections affecting fish and other poikilothermic and homeothermic animals (Shotts, Gaines, Martin & Prestwood 1972;Boulanger, Lallier & Cousineau 1977;De Figueiredo & Plumb 1977;Larsen & Jensen 1977;Rahim, Sanyal, Aziz, Huq & Chowdhury 1984;Toranzo, Combarro, Conde & Baija 1985). They are also considered autochthonous inhabitants of aquatic environments, being isolated from both polluted and unpolluted waters throughout the world (Hazen, Fliermans, Hirsch & Esch 1978;Kaper, Lockman, Colwell & Joseph 1981;Larsen & Willeberg 1984).…”

Section: Introductionmentioning

confidence: 99%

Association of Aeromonas sobria with mortalities of adult gizzard shad, Dorosoma cepedianum Lesueur

Toranzo

Baya

Romalde

et al. 1989

Journal of Fish Diseases

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This report describes an epizootic in a natural population of spawning females of gizzard shad, Dorosoma cepedianum Lesueur, which occurred in the Potomac River (Maryland, USA). Microbiologieal analysis of moribund shad revealed the presence in pure culture in all the organs examined of one bacterium which was identified as Aeromonas sobria by conventional tube and plate tests. The different API-systems used to attempt for a rapid diagnosis of the disease yielded false negative and positive reactions in important differential tests such as ornithine decarboxylase, arabinose fermentation, gelatinase, urease, indole and production of H^S which gave an erroneous identifieation of the shad isolate. The virulence assays in rainbow trout revealed that A. sobria was pathogenic with a mean LDso of 2 X 10^^. Bacterial extracellular products a)so displayed fish lethality and were cytotoxic for poikilothermic and homcothermic cell-lines. Although the source of shad infection is unknown, the physico-chemical conditions of the river, together with the stress associated with the spawning could have been contributing factors in the proliferation of A. sobria indueing this epizootic.

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Isolation of enterotoxigenic, hemolytic, and antibiotic-resistant Aeromonas hydrophila strains from infected fish in Bangladesh

Cited by 59 publications

References 17 publications

Role of LuxR‐type regulators in fish pathogenic Aeromonas hydrophila

Role of LuxR‐type regulators in fish pathogenic Aeromonas hydrophila

Antibiotic resistance pattern of motile aeromonads from farm raised fresh water fish

Association of Aeromonas sobria with mortalities of adult gizzard shad, Dorosoma cepedianum Lesueur

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