Isolation of epithelial cell types from the rat colon

Chopra, Dharam P.; Yeh, Kwo–Yih; Gordon, Deborah G.

doi:10.1007/bf00920579

Cited by 7 publications

(9 citation statements)

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“…Isolation and culture of solid tumor cells under in vitro environment similar to the microenvironment of the original tumor is a challenge and requires specialized techniques [11, 12]. Successful isolation of tumor cells with suitable technologies is critically dependent upon an appropriate method to disrupt the extracellular matrix, which consists of a complex mixture of cohesive factors among constitutive proteins [13, 14].…”

Section: The Need For Primary Tumor Cell Linesmentioning

confidence: 99%

Technologies for deriving primary tumor cells for use in personalized cancer therapy

Mitra

Mishra

2013

Trends in Biotechnology

175

162

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For decades, immortal cancer cell lines have constituted an accessible, easily usable set of biological models to investigate cancer biology and explore the potential efficacy of anticancer drugs. However, numerous studies suggest that these cell lines poorly represent the diversity, heterogeneity and drug-resistant tumors occurring in patients. The derivation and short -term culture of primary cells from solid tumors have thus gained significant importance in personalized cancer therapy. This review focuses our current understanding and the pros and cons of different methods for primary tumor cell culture. Furthermore, various culture matrices like biomimetic scaffolds, chemically defined media supplemented with essential nutrients have been prepared for different tissues. These well characterized primary tumor cells redefine cancer therapies with high translational relevance.

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Section: The Need For Primary Tumor Cell Linesmentioning

confidence: 99%

Technologies for deriving primary tumor cells for use in personalized cancer therapy

Mitra

Mishra

2013

Trends in Biotechnology

175

162

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“…In addition, such epithelial cell lines, which retained many phenotypes of the colon cells. Trypsin was also employed to isolate various types of colonocytes (ie, proliferative, mucous, and absorptive) according to their location along the villus-crypt axis, by repeated time dissociation of colonic mucosa of adult rat [47]. Quaroni et al also used collagenase to isolate and culture epithelial cells from small intestine of rat [8] and pig [9].…”

Section: Organ Culturementioning

confidence: 99%

Intestinal Epithelial Cells In Vitro

Chopra

Dombkowski

Stemmer

et al. 2010

Stem Cells and Development

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Recent advances in the biology of stem cells has resulted in signifi cant interest in the development of normal epithelial cell lines from the intestinal mucosa, both to exploit the therapeutic potential of stem cells in tissue regeneration and to develop treatment models of degenerative disorders of the digestive tract. However, the diffi culty of propagating cell lines of normal intestinal epithelium has impeded research into the molecular mechanisms underlying differentiation of stem/progenitor cells into the various intestinal lineages. Several short-term organ/organoid and epithelial cell culture models have been described. There is a dearth of long-term epithelial and/or stem cell cultures of intestine. With an expanding role of stem cells in the treatment of degenerative disorders, there is a critical need for additional efforts to develop in vitro models of stem/progenitor epithelial cells of intestine. The objective of this review is to recapitulate the current status of technologies and knowledge for in vitro propagation of intestinal epithelial cells, markers of the intestinal stem cells, and gene and protein expression profi les of the intestinal cellular differentiation.

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“…In addition to containing smaller populations of endocrine cells, the colonic mucosa appears to be composed of seemingly homogeneous populations of epithelial colonocytes and goblet cells (1)(2)(3)(4). Despite the recognition that alterations may exist in goblet cell glycoproteins in association with a variety of disease processes (5-1 1), there is little basic understanding of normal goblet cell function (12)(13)(14).…”

Section: Introductionmentioning

confidence: 99%

Human colonic goblet cells. Demonstration of distinct subpopulations defined by mucin-specific monoclonal antibodies.

1986

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We studied glycoprotein content of human colonic goblet cells, using a library of monoclonal antibodies (MAbs) Anti-HCM MAbs stained goblet cells from other sites within the gastrointestinal tract to a varying extent. Anti-HCM MAbs also showed extensive cross-reactivity with rodent, rabbit, and monkey colonic mucosa. However, several anti-HCM MAbs stained only human colonic mucosa. These data show that human colonic mucosa contains discrete subpopulations of goblet cells that produce distinctive combinations of specific mucin glycoprotein species.

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Isolation of epithelial cell types from the rat colon

Cited by 7 publications

References 1 publication

Technologies for deriving primary tumor cells for use in personalized cancer therapy

Technologies for deriving primary tumor cells for use in personalized cancer therapy

Intestinal Epithelial Cells In Vitro

Human colonic goblet cells. Demonstration of distinct subpopulations defined by mucin-specific monoclonal antibodies.

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