Isolation of erythropoietin-sensitive cells from Friend virus-infected marrow cultures: characteristics of the erythropoietin response

Bondurant, Maurice C.; Koury, Mark J.; Krantz, Sanford B.; Blevins, Teresa; Duncan, Dexter T.

doi:10.1182/blood.v61.4.751.751

Cited by 23 publications

(9 citation statements)

References 7 publications

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“…These findings are not due to inefficient immunoprecipitation since control experiments have shown that the antisera precipitate virtually all of the spectrins and band 3 from preparations of mature erythrocyte membranes. That the spectrins and band 3 represent very small percentages of total protein synthesis is also supported by 2-D gel analyses of newly synthesized proteins from whole cells (Bondurant et al, 1983; our unpublished data of 2D gels for all cell fractions).…”

Section: Synthesis and Assembly Of Spectrins And Band 3 Into Erythrobsupporting

confidence: 57%

Changes in erythroid membrane proteins during erythropoietin‐mediated terminal differentiation

Koury

Bondurant

Rana

1987

Journal Cellular Physiology

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Membrane and membrane skeleton proteins were examined in erythroid progenitor cells during terminal differentiation. The employed model system of erythroid differentiation was that in which proerythroblasts from mice infected with the anemia-inducing strain of Friend virus differentiate in vitro in response to erythropoietin (EP). With this system, developmentally homogeneous populations of cells can be examined morphologically and biochemically as they progress from proerythroblasts through enucleated reticulocytes. alpha and beta spectrins, the major proteins of the erythrocyte membrane skeleton, are synthesized in the erythroblasts both before and after EP exposure. At all times large portions of the newly synthesized spectrins exist in and are turned over in the cytoplasm. The remaining newly synthesized spectrin is found in a cellular fraction containing total membranes. Pulse-chase experiments show that little of the cytoplasmic spectrins become membrane associated, but that the proportion of newly synthesized spectrin which is membrane associated increases as maturation proceeds. A membrane fraction enriched in plasma membranes has significant differences in the stoichiometry of spectrin accumulation as compared to total cellular membranes. Synthesis of band 3 protein, the anion transporter, is induced only after EP addition to the erythroblasts. All of the newly synthesized band 3 is membrane associated. A two-dimensional gel survey was conducted of newly synthesized proteins in the plasma membrane enriched fraction of the erythroblasts as differentiation proceeded. A majority of the newly synthesized proteins remain in the same proportion to each other during maturation; however, a few newly synthesized proteins greatly increase following EP induction while others decrease markedly. Of the radiolabeled proteins observed in two dimensional gels, only the spectrins, band 3 and actin become major proteins of the mature erythrocyte membrane. Examination of total proteins of the plasma membrane enriched fractions of EP-treated erythroblasts using silver staining and 32P autoradiography show that many proteins and phosphoproteins are selectively eliminated from this fraction late in the course of differentiation during the reticulocyte stage. The selective removal of many proteins at the reticulocyte stage of development combined with previous selective synthesis and accumulation of some specific proteins such as alpha and beta spectrin and band 3 in the differentiating erythroblasts lead to the final mammalian erythrocyte membrane structure.

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Section: Synthesis and Assembly Of Spectrins And Band 3 Into Erythrobsupporting

confidence: 57%

Changes in erythroid membrane proteins during erythropoietin‐mediated terminal differentiation

Koury

Bondurant

Rana

1987

Journal Cellular Physiology

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“…It has previously been reported that both hypoxia and CoC1, increase expression of Epo mRNA (21,22) and production of biologically active Epo protein (22,36). Similar results have been found for kidney tissue, indicating that tissue hypoxia, cobalt exposure or both can specifically enhance Epo gene expression in these cell types (37).…”

Section: Discussionsupporting

confidence: 72%

Coexpression of erythropoietin and heme oxygenase genes in Hep3B cells

et al. 1993

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Exposure of Hep3B cells to metalloporphyrins (tinprotoporphyrin and heme) or cobalt chloride resulted in the production of a significant number of heme oxygenase transcripts, erythropoietin transcripts or both, as indicated by in situ hybridization. Exposure to heme 10 mumol/L resulted in a 30-fold to 40-fold increase in cells expressing erythropoietin messenger RNA (erythropoietin-positive cells) by 6 hr; this increased level remained elevated for 24 hr. Tin-protoporphyrin (10 mumol/L) produced an eightfold to 10-fold increase in erythropoietin RNA within 40 min. This value then returned to control levels by 60 min. Exposure to cobalt chloride (100 mumol/L) resulted in a 20-fold to 30-fold increase in erythropoietin expression for 5 to 20 min, returning to control by 40 min. Additionally, nuclear runoff assays demonstrated that the increase in heme oxygenase or erythropoietin messenger RNA accumulation by cobalt chloride appeared to be a result of stimulated transcription of the heme oxygenase and erythropoietin genes. However, the pattern for heme oxygenase messenger RNA induction was different from that for erythropoietin expression. Heme produced an immediate expression of heme oxygenase RNA (50-fold within 5 min) and a second sustained response during the next 24 hr. Tin-protoporphyrin also produced an immediate response (40-fold within 5 min) and remained elevated (20-fold) for 6 hr. Cobalt chloride produced a 22-fold increase within 20 min and returned to the control value by 1 hr. Thus both erythropoietin and heme oxygenase genes appear to be expressed after treatment with tin-protoporphyrin, heme or cobalt chloride; however, the time and patterns of expression are different.(ABSTRACT TRUNCATED AT 250 WORDS)

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“…The difference in rates of erythroid gene transcription between PHZ and FVA cells may result from slight differences in the stage of differentiation and heterogeneity of the starting populations. Although various studies have been published on gene expression during FVA cell differentiation (Bondurant et al, 1983(Bondurant et al, , 1985Koury et al, 1986;Wickrema et al, 1991), this is the ®rst study to use PHZ cells.…”

Section: Discussionmentioning

confidence: 99%

“…The FVA model utilizes cells isolated from the spleens of mice treated with SFFVa. Following infection, the spleen increases markedly in size and contains erythroblasts which fail to progress as far as haemoglobin synthesis and erythrocyte maturation (Bondurant et al, 1983). These cells are produced during the ®rst phase of SFFVa virus infection prior to malignant transformation and in primary explant culture EPO induces their differentiation into fully enucleated reticulocytes.…”

mentioning

confidence: 99%

Erythroblasts from Friend virus infected‐ and phenylhydrazine‐treated mice accurately model erythroid differentiation

Hodges¹,

Winter²,

Lappin³

1999

Br J Haematol

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Summary. The dynamics of gene expression during terminal erythroid differentiation have been examined in three murine models; the erythroleukaemia cell line HCD-57 and splenic erythroblasts isolated from mice treated with either the anaemia-inducing strain of Friend virus (FVA cells) or the haemolytic agent phenylhydrazine (PHZ cells). In response to erythropoietin (EPO) and haemin, HCD-57 cells proliferated and synthesized haemoglobin, but failed to complete terminal differentiation as indicated by lack of change in both gene expression and morphological appearance. In contrast, EPO-induced terminal differentiation in FVA and PHZ cells in vitro was accompanied by increases in haemoglobin positivity, morphological maturation and a shared pattern of gene expression. EPO receptor (EPO-R) mRNA levels peaked before globin gene expression which was maximal at 24 h. Peak GATA-1 and EKLF mRNA levels also preceded the globin gene peak, but the highest NF-E2 levels coincided with maximal globin levels, suggesting a role for NF-E2 in the maintenance, rather than the initiation of globin gene expression. Peak expression of d-aminolaevulinic acid synthase (ALAS) coincided with peak globin expression. FVA and PHZ cells represent more effective models than the HCD-57 cell line for the investigation of erythroid gene expression during EPO-regulated terminal erythropoiesis.

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Isolation of erythropoietin-sensitive cells from Friend virus-infected marrow cultures: characteristics of the erythropoietin response

Cited by 23 publications

References 7 publications

Changes in erythroid membrane proteins during erythropoietin‐mediated terminal differentiation

Changes in erythroid membrane proteins during erythropoietin‐mediated terminal differentiation

Coexpression of erythropoietin and heme oxygenase genes in Hep3B cells

Erythroblasts from Friend virus infected‐ and phenylhydrazine‐treated mice accurately model erythroid differentiation

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