Isolation of ESBL-producing gram-negative bacteria and in silico inhibition of ESBLs by flavonoids

Swain, Shasank S.; Padhy, Rabindra N.

doi:10.1016/j.jtumed.2016.03.007

Cited by 4 publications

(2 citation statements)

References 32 publications

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“…27 Another study revealed that Nitrofurantoin was the most efficient antibiotic. 28 According to previousreports, the resistance against antibiotics from 2005 to 2014 was similar to the present study. 29 A recent study in China conducted showed that the resistance proportion was consistent with both gramnegative bacteria and gram-positive bacteria.…”

Section: Discussionsupporting

confidence: 92%

Frequency of bacterial isolates and antibiotics resistance patterns in urine and pus samples.

Ali

Khalid

Muhammad

et al. 2021

TPMJ

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Objectives: The present study is aimed to determine frequency of bacteria in urine and pus sample along with the antibiotic resistance profile of isolated bacteria. Study Design: Cross-Sectional Study. Setting: Real-Time PCR Laboratory at Dabgari Garden, Peshawar with the Collaboration of Health Biotechnology Laboratory, Department of Biotechnology, Abdul Wali Khan University, Mardan. Period: June 2018 to July 2019. Material & Methods: The urine and pus samples were collected from suspected patients and were cultured on appropriate culture media. The biochemical tests were also performed after growing on culture plates for identification and confirmation of bacterial isolates. The disc diffusion technique was used to evaluate the antibiotic pattern of retrieved isolates. Result: A total of 525 samples of pus and urine samples were collected from different regions of Peshawar. Out of the total, 237 (45.1%) samples were found positive for bacterial growth whereas the remaining (54.9%) were observed negative. Among total positive isolates, 220 (92.8%) were from urine samples, and 17 (7.2%) were from pus samples. The predominant isolate was Escherichia coli (E. coli) (90.3%) retrieved from positive samples, followed by Pseudomonas aeruginosa (7.2%), Klebsiella pneumonia (1.3%), and Staphylococcus aureus (1.3%). The most effective antibiotic was Fosfomycin against bacteria whereas Nalidixic acid, Nitrofurantoin, and Amoxicillin/clavulanic acid were found less effective against bacterial isolates. Conclusion: Most frequent bacteria isolated was E. coli and the most efficient drug was Fosmomycin and the least was Nitrofurantoin, and Amoxicillin.

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Section: Discussionsupporting

confidence: 92%

Frequency of bacterial isolates and antibiotics resistance patterns in urine and pus samples.

Ali

Khalid

Muhammad

et al. 2021

TPMJ

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“…In addition, in Asian and African regions, massive amounts of aromatic medical plants have been identified, but most medical applications are still unknown or limited, with some regional ethnomedicinal use without proper scientific investigations. Currently, the drug screening procedure has been revolutionized through high-through instrumental and artificial intelligence-based screening, which is more beneficial to explore the potential therapeutic potency of each plant species along with derived secondary metabolites (Swain and Padhy, 2016;Sahoo et al, 2021;Sahoo et al, 2022a andSwain et al, 2022a).…”

Section: Introductionmentioning

confidence: 99%

Alcoholic Extracts of Eleusine indica as Alternative Diuretic Regimens: A Computational Based Investigation

Sahu,

Sahoo,

Pattnaik

et al. 2024

IJERR

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Diuretics are widely used in current clinical practice to increase urine production and excrete electrolytes, particularly sodium and chloride ions, without affecting the absorption of protein, vitamins, carbohydrates, or amino acids. From the time of mercury chloride and organomercurials in ancient times to now (with sulphonamides, thiazides, and furosemide), a lot has changed in the field of diuretics. However, long-term use of such synthetic diuretic agents in clinical practice produces several adverse effects, such as blurred vision, loss of appetite, stomach upset, carcinomas, headaches, phototoxic impact, weakness., etc., as has been observed from recent investigations. Natural regimens can serve as potential alternatives to using nontoxic diuretic agents. Based on long-term ethnomedicinal and biological activity records, we have explored the diuretic effects of the widely known perennial herb in Pacific Islands regions and a weed in agricultural fields, Eleusine indica (L) Gaertn phytoconstituents, on a computation platform. Therefore, we conducted a bio-assay-guided crude extraction using ethanol, followed by further gas chromatography-mass spectrometry (GC-MS) analyses of the extracted crude extracts. Further selected nine constituents (EI_1 to EI_9) carried out the diuretic potency against three putative target enzymes (ACE, KCNJ1, and SLC12A1) along with three standard drugs (VU590, TSM, and FSM) through molecular docking studies using AutoDock 4.2 software. We also predict physicochemical profiles, or Lipinski Rule of Five profiles, toxicity, and pharmacokinetics using various bioinformatics and cheminformatics tools. Based on the overall investigation, it was revealed that EI_6 [Z, Z-6,28-Heptatriacontadien-2-one] was the most potential, nontoxic, and drug-able candidate. In summary, advanced computational tools play a crucial role in selecting potential preclinical candidates within limited resources to accelerate the current drug discovery process.

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Antibiotic-Resistant Klebsiella pneumoniae and Targeted Therapy

Verma

Semalty

Gabrani

2022

Antimicrobial Resistance

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Isolation of ESBL-producing gram-negative bacteria and in silico inhibition of ESBLs by flavonoids

Cited by 4 publications

References 32 publications

Frequency of bacterial isolates and antibiotics resistance patterns in urine and pus samples.

Frequency of bacterial isolates and antibiotics resistance patterns in urine and pus samples.

Alcoholic Extracts of Eleusine indica as Alternative Diuretic Regimens: A Computational Based Investigation

Antibiotic-Resistant Klebsiella pneumoniae and Targeted Therapy

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