Isolation of Escherichia Coli 0157:h7 From Dairy Cattle Associated With Two Cases of Haemolytic Uraemic Syndrome

Martin, Mary Lane; Shipman, LarryD; Potter, M. D.; Wachsmuth, I K; Wells, JoyG; Hedberg, Katrina; Tauxe, R. V.; Davis, J M; Arnoldi, J.; Tilleli, John

doi:10.1016/s0140-6736(86)92656-5

Cited by 176 publications

(7 citation statements)

References 4 publications

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“…Overall criteria for diagnosis UTI was growth of ≥10 4 CFU/mL of a urinar y tract pathogen) in patients who did not have another potential source for their fever, a history of UTI, malodorous urine or hematuria, appeared "ill", abdominal or suprapubic tenderness on examination, or fever ≥39°C [25]. Patients who had received antibiotic treatment within the preceding 2 weeks, and those with known congenital anomalies of urinary tract or central nervous system-associated anomalies were excluded.…”

Section: Methodsmentioning

confidence: 99%

Study Prevalence of Verotoxigenic E.coli Isolated from Urinary Tract Infections (UTIs) in an Iranian Children Hospital

Navidinia

Karimi²,

Rahbar³

et al. 2012

TOMICROJ

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Background and Objectives:Urinary tract infections (UTI) caused by enterohemorrhagic Escherichia coli (EHEC) is one of the most important diseases in infants and children. If there would not be any useful diagnosis and treatment it may be resulted in diseases such as acute renal failure, thrombocytopenia and hemolytic anemia. The aim of this study was to determine frequency of verotoxigenic E.coli isolates in urine of children with (UTIs) in Mofid children Hospital.Methods:During one year from September 2008 to august 2009, urine specimens were taken from children who suspected to UTI admitted to Mofid Children Hospital. E.coli strains that indicated beta hemolytic on sheep blood agar, negative sorbitol fermentation on SMAC (sorbitol macconky agar) and negative motility on SIM were tested by PCR and serologic (VITEC-RPLA kit) methods for detecting toxin genes and production of toxin, respectively.Results:Among 12572 urine specimens were taken from children admitted to Mofid hospital, we isolated 378 E.coli from urine samples which only 9 isolates were EHEC. Only five EHEC strains (55%) which produced vtx genes, were detected by serologic and PCR methods.Conclusion:The prevalence of urinary infections caused by EHEC strains is very significant because it causes aggravating pathologic effects. Thus we suggest rapid method for identification of this bacteria and proper treatment to Inhibition of unwanted complications.

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Section: Methodsmentioning

confidence: 99%

Study Prevalence of Verotoxigenic E.coli Isolated from Urinary Tract Infections (UTIs) in an Iranian Children Hospital

Navidinia

Karimi²,

Rahbar³

et al. 2012

TOMICROJ

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“…Whereas EHEC were initially linked to cases and outbreaks caused by consumption of raw or undercooked beef products, it is now apparent that many different types of products that are produced without a bacterial control step (e.g., heat treatment) can transmit this pathogen, including produce, raw apple cider, raw milk, and certain raw milk dairy products (for a comprehensive review of STEC in dairy foods, see Farrokh et al, 2013). In 1986, 2 cases of E. coli O157:H7 infections were described in young children who consumed raw milk on farms where E. coli O157:H7 was subsequently also isolated from fecal samples from dairy cows, suggesting raw milk as a potential vehicle responsible for human O157:H7 infections (Martin et al, 1986). An early report of a linkage between EHEC and dairy products was also provided by Deschênes et al (1996), who reported 4 HUS cases in a French village between March 1992 and May 1993 that were associated with consumption of a raw milk cheese.…”

Section: To 1992: Enterobacter Sakazakii and Stec Are Recognized As Causes Of Dairy-associated Foodborne Disease Outbreaks-a Reminder Thamentioning

confidence: 99%

“…Two cases of Escherichia coli O157:H7 infections are described in children who consumed raw milk on farms where E. coli O157:H7 was subsequently isolated from fecal samples from dairy cows, suggesting raw milk as a potential vehicle for human O157:H7 infections. Martin et al, 1986Martin et al, 1986Martin et al, -1987 Three cases of neonatal infection caused by Enterobacter sakazakii (now named Cronobacter) are identified in a hospital in Reykjavík, Iceland; these appear to be the first Cronobacter cases definitively linked to infant formula. Biering et al, 1989Biering et al, 1987 The US Food and Drug Administration (FDA) publishes a final regulation mandating the pasteurization of all milk and milk products in final package form for direct human consumption; this regulation banned the shipping of raw milk in interstate commerce.…”

Section: Appendixmentioning

confidence: 99%

A 100-Year Review: Microbiology and safety of milk handling

Boor

Wiedmann

Murphy

et al. 2017

Journal of Dairy Science

153

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Microbes that may be present in milk can include pathogens, spoilage organisms, organisms that may be conditionally beneficial (e.g., lactic acid bacteria), and those that have not been linked to either beneficial or detrimental effects on product quality or human health. Although milk can contain a full range of organisms classified as microbes (i.e., bacteria, viruses, fungi, and protozoans), with few exceptions (e.g., phages that affect fermentations, fungal spoilage organisms, and, to a lesser extent, the protozoan pathogens Cryptosporidium and Giardia) dairy microbiology to date has focused predominantly on bacteria. Between 1917 and 2017, our understanding of the microbes present in milk and the tools available for studying those microbes have changed dramatically. Improved microbiological tools have enabled enhanced detection of known microbes in milk and dairy products and have facilitated better identification of pathogens and spoilage organisms that were not known or well recognized in the early 20th century. Starting before 1917, gradual introduction and refinement of pasteurization methods throughout the United States and many other parts of the world have improved the safety and quality of milk and dairy products. In parallel to pasteurization, others strategies for reducing microbial contamination throughout the dairy chain (e.g., improved dairy herd health, raw milk tests, clean-in-place technologies) also played an important role in improving microbial milk quality and safety. Despite tremendous advances in reducing microbial food safety hazards and spoilage issues, the dairy industry still faces important challenges, including but not limited to the need for improved science-based strategies for safety of raw milk cheeses, control of postprocessing contamination, and control of sporeforming pathogens and spoilage organisms.

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“…Most cases of disease have been sporadic , yet outbreaks have occurred in nursing homes, fast food restaurant s, banquets and day care centers (6,19,21,54). Raw milk has been implicated as the vehicle for several cases in 1986 in which E. coli was isolated from feces from healthy heifers who supplied raw milk to two of the case patients (55). Dairy cattle have been shown to harbor E. coli 0157:H7 more commonly than beef cattle (55).…”

Section: Epidemiologymentioning

confidence: 99%

Verotoxic Escherichia coli in Human Disease

Rosensweig,

Gourley

1991

J. pediatr. gastroenterol. nutr.

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SummaryVerotoxin‐producing E. coli (most frequently E. coli 0157) have been implicated in the pathogenesis of diarrhea, hemorrhagic colitis, hemolytic uremic syndrome and thrombotic thrombocytopenic purpura. Cattle, meat products, and other sources have been found to harbor these organisms. Isolation of E. coli 0157:H7 on MacConkey‐sorbitol agar is diagnostic, yet the bacteria are difficult to detect after the first week of infection. Enzyme linked immunosorbent assays (ELISAs) can detect verotoxin in fecal filtrates in the absence of viable bacteria. Serologic evidence has also been used to support the diagnosis of verotoxin‐associated infection. Evidence supporting the etiologic role of different verotox‐ins is reviewed. Treatment remains supportive since the use of antibiotics and antimotility agents can lead to poorer outcomes. Recommendations for prevention are presented.

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Isolation of Escherichia Coli 0157:h7 From Dairy Cattle Associated With Two Cases of Haemolytic Uraemic Syndrome

Cited by 176 publications

References 4 publications

Study Prevalence of Verotoxigenic E.coli Isolated from Urinary Tract Infections (UTIs) in an Iranian Children Hospital

Study Prevalence of Verotoxigenic E.coli Isolated from Urinary Tract Infections (UTIs) in an Iranian Children Hospital

A 100-Year Review: Microbiology and safety of milk handling

Verotoxic Escherichia coli in Human Disease

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