Isolation of Escherichia coli O128: HNM Harboring stx2f Gene from Diarrhea Patients

Isobe, Junko; Kimata, Keiko; Shimojima, Masayuki; Hosorogi, Shiho; Tanaka, Daisuke; Gyobu, Yotaku

doi:10.11150/kansenshogakuzasshi1970.78.1000

Cited by 14 publications

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“…The present identification of an STEC 2f isolate from a swallow is the first report in which an STEC strain was isolated from a wild bird other than a dove. According to previous reports, STEC 2f isolates from wild birds or the infant can be classified into one of two subtypes: O128 and O45 (10,14,22). However, in the present study STEC 2f strains classified as O132, O20, and O147 were detected.…”

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“…Moreover, four of the stx 2f -positive strains were confirmed for their expression of class Stx2 by the verocytotoxin-producing E. coli reverse passive latex agglutination test (Seiken, Tokyo, Japan). The presence of STEC 2f strains in wild birds has only been described in a few reports, and to date, these strains have only been isolated from the bird order Columbiformes (doves and pigeons) and a human infant (10,14,22). The present identification of an STEC 2f isolate from a swallow is the first report in which an STEC strain was isolated from a wild bird other than a dove.…”

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Prevalence and Characteristics of eae - and stx -Positive Strains of Escherichia coli from Wild Birds in the Immediate Environment of Tokyo Bay

Kobayashi

Kanazaki

Hata

et al. 2009

Appl Environ Microbiol

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The prevalence and characteristics of eae-and stx-positive Escherichia coli strains in wild birds in the immediate environment of Tokyo Bay, Japan, was examined using cloacal swab samples taken from 447 birds belonging to 62 species. PCR screening showed that the prevalences of stx-and eae-positive strains of Escherichia coli were 5% (23/447) and 25% (113/447), respectively. Four strains of stx 2f -positive E. coli were isolated from two feral pigeons, an oriental turtle dove and a barn swallow. In contrast, 39 eae-positive E. coli strains were isolated, and most of the strains possessed a subtype of intimin that is classified as a minor group of human intimins, such as intimin , , and . Moreover, these strains did not possess any of the other pathogenic genes tested, such as stxs, ehxA, bfp, or irp. Thus, wild birds were considered to be a reservoir of atypical enteropathogenic E. coli.Pathogenic Escherichia coli, such as enterohemorrhagic E. coli (EHEC), enteropathogenic E. coli (EPEC), and attaching and effacing E. coli, are food-borne pathogens that cause diarrhea in humans (13,16,20). The pathogenicity of these E. coli strains is, in large part, due to the fact that they express genes for Shiga toxins (stx genes) and/or for intimin, a virulence factor that is an outer membrane protein (eae). Ruminants are considered to be the main reservoir of Stx-producing E. coli (STEC). However, other domestic animals such as goats, pigs, poultry, cats, and dogs can also harbor STEC, in addition to intimin-producing E. coli (3,4). A recent investigation of the occurrence of STEC in wild birds (14,18,22,24) indicated that the latest stx 2 variant (stx 2f ) is found in pigeons (22); however, the carriage of stx-or eae-possessing E. coli strains in wild animals has not been thoroughly investigated. Some wild birds live in human habitats and others migrate between waste treatment plants, marketplaces, cattle pastures, and pig farms. Such interactions between humans and wild birds therefore make birds important vehicles for the spread of zoonotic infections.The goal of the present study was to analyze the role of wild birds as reservoirs of stx-and intimin-producing strains of E. coli. The prevalence of these E. coli strains in wild birds was examined using PCR analysis, and further genetic characterization of the various virulence genes of the isolated strains was performed.The E. coli strain O157:H7, used as a positive-control strain for analysis of the stx 1 , stx 2 , and eae genes by PCR, was obtained from the American Type Culture Collection (ATCC 35150). The E. coli strains Ch05031 and PGN28, used as positive-control strains in PCR analyses of the stx 2e and stx 2f genes, respectively, were derived from the stock culture collection of the National Institute of Animal Health, Japan. A total of 447 cloacal swab samples were collected from 62 species of wild birds that had been captured between 2003 and 2007 (Table 1). The birds were captured for various reasons, such as falling from nests or as a consequence of being hunt...

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confidence: 63%

Prevalence and Characteristics of eae - and stx -Positive Strains of Escherichia coli from Wild Birds in the Immediate Environment of Tokyo Bay

Kobayashi

Kanazaki

Hata

et al. 2009

Appl Environ Microbiol

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“…The four O63:H6 strains in Osaka Prefecture produced Stx2, and had stx2f and intimin α genes. STEC O128 strains harboring stx2f were isolated from pigeons and infant patients [14,21,39]. In addition, intimin α was found frequently in enteropathogenic E. coli (EPEC) [33] and it was detected in only two STEC O177:H7 isolates in Germany [5].…”

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confidence: 99%

Biochemical and Molecular Characterization of Minor Serogroups of Shiga Toxin-Producing Escherichia coli Isolated from Humans in Osaka Prefecture

Seto

Taguchi

Kobayashi

et al. 2007

The Journal of Veterinary Medical Science

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ABSTRACT. We have investigated 37 minor serogroup Shiga toxin-producing Eschrichia coli (STEC) strains other than O157, O26, and O111 isolated from human specimens in Osaka prefecture to determine their serological and biochemical characteristics, virulence-associated genes, and clinical signs in patients. The same serotype strains were genotyped by pulsed-field gel electrophoresis (PFGE). The O antigen of 33 strains were typed into 10 serogroups; O28, O63, O65, O91, O103, O119, O121, O126, O165, and O177, and other 4 strains were not agglutinated with any serum. Four different Shiga toxin (Stx) types (1, 2, 2c, and 2f) were distributed in these isolates.The intimin gene was present in 83.8% of the strains and subtyped into intimin α, β, ε, and ζ. STEC O165, O177, and OUT isolated from hemolytic uremic syndrome (HUS) patients revealed atypical biochemical characters; negative reaction for lysine decarboxylase and gas production from glucose. Eleven strains including the isolates from HUS patients generated no colonies on cefixime-tellurite (CT)-sorbitol-MacConkey agar plates, since they showed high sensitivity (MIC ≤ 1.25 µg/ml) to potassium tellurite. The finding show supportive information for use the selective agar plates with and without CT supplement for the isolation of minor serogroup STEC. PFGE analysis revealed that the strains isolated from family cases were closely related within the respective family, and it was useful for epidemiological analysis of minor serogroup STEC.

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“…Although all STEC strains in this study only possessed the stx2f subtype, the public health significance of these strains should not be overlooked, because the stx2f+ E. coli strains have also been isolated from humans with diarrhea (Prager et al, 2009;Isobe et al, 2004). Recent evidences also show the particular importance of stx2f-STEC as an emerging unnoticed human pathogen (Friesema et al, 2014).…”

Section: Prevalence Of Shiga Toxin-producing and Enteropathogenic Escmentioning

confidence: 99%

Prevalence of Shiga Toxin-Producing and Enteropathogenic Escherichia coli in Wild and Pet Birds in Iran

Koochakzadeh

Badouei

Salehi

et al. 2015

Rev. Bras. Cienc. Avic.

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The aim of this study was to investigate the prevalence of Shiga toxin-producing Escherichia coli (STEC) and enteropathogenic E. coli (EPEC) strains and to identify the stx gene types in wild captive and companion birds. In total,657 E. coli isolates from 219 birds belonging to 38 different species were investigated for the presence of STEC and EPEC strains. It was shown that five birds (2.28%) carried strains positive for one or more of the virulence factors investigated. The results indicated that 1.8% (n=4) and 0.45% (n=1) of the birds carried STEC and EPEC strains, respectively. All STEC strains harbored the stx2f and eae genes and this finding reveals the role of other birds, in addition to pigeons, as reservoirs of STEC. The only EPEC strain in this study was isolated from a Myna. Based on our knowledge, this is the first report of Stx2f-producing STEC in Geese, Duck and Lesser kestrel. In conclusion, the results indicate a low frequency of STEC carriage in wild and companion birds, and point out the need of additionally screening for the presence of stx2f in all the eae-harboring strains from birds.

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Isolation of Escherichia coli O128: HNM Harboring stx2f Gene from Diarrhea Patients

Cited by 14 publications

References 0 publications

Prevalence and Characteristics of eae - and stx -Positive Strains of Escherichia coli from Wild Birds in the Immediate Environment of Tokyo Bay

Prevalence and Characteristics of eae - and stx -Positive Strains of Escherichia coli from Wild Birds in the Immediate Environment of Tokyo Bay

Biochemical and Molecular Characterization of Minor Serogroups of Shiga Toxin-Producing Escherichia coli Isolated from Humans in Osaka Prefecture

Prevalence of Shiga Toxin-Producing and Enteropathogenic Escherichia coli in Wild and Pet Birds in Iran

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