Isolation of Extracellular Vesicles from Stem Cells

Abstract

Transplanted induced pluripotent stem cells (IPSC) and embryonic stem cells (ESC) exhibit enhanced survival in ischemic tissues and promote survival of neighboring cells via paracrine effects. Recent studies indicate that stem cells can secrete extracellular vesicles (EV), which can shuttle noncoding RNA between cells and facilitate intercellular signaling and communication between donor stem cells and recipient tissues. Direct transplantation of IPSC-derived EV (IPSC-EV) is highly effective at promoting survival and preventing apoptosis of cardiomyocytes in a mouse model of acute myocardial ischemia–reperfusion (MI/R). Here, we describe a feasible protocol to purify EV from cultured IPSC.

Transplantation of Cardiac Mesenchymal Stem Cell-Derived Exosomes Promotes Repair in Ischemic Myocardium

“…Exosomes/microvesicles secreted by C-MSCs were purified from conditioned media as previously described with modification(29, 34). Briefly, culture medium containing 10% exosome-depleted FBS was added to C-MSC.…”

Transplantation of Cardiac Mesenchymal Stem Cell-Derived Exosomes for Angiogenesis

“…Exosomes/microvesicles secreted by C-MSCs were purified from conditioned media by polymer-based exosome precipitation protocol as we described previously [19, 28, 29]. Briefly, C-MSC were cultured in culture medium containing 10% exosome-depleted FBS for 48 hours, collected media was centrifuged at 1000 rpm for 10 minutes, and then filtered through a 0.22 μm filters.…”

Extracellular vesicle and mesenchymal stem cells in bone regeneration: recent progress and perspectives

“…Sucrose gradient centrifugation, based on the most commonly used sucrose medium, achieves native morphology and higher purity, but the long run time and complicated operation could not be neglected . Besides, two‐aqueous phase separation system based on the principle that different particles are separated to different phases achieved higher yield and higher purity …”