Isolation of Fallopian Tube Epithelium for Assessment of Cilia Beating Frequency (CBF)

Russo, Angela; Burdette, Joanna E.

doi:10.1007/978-1-0716-1956-8_12

Cited by 3 publications

(1 citation statement)

References 20 publications

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“…Additionally, the reduction is much lower than estrogen leaving an unbalanced ratio of estrogen/testosterone levels that could be even more pronounced locally at the ovary-FTE interface. This question could be investigated using a microfluidic system that allows studying ovulation in real time as previously described [ 53 , 54 ]. In summary, our results are the first to show how local stimulation of FTE with testosterone can affect the function of these cells and render them more migratory and invasive.…”

Section: Discussionmentioning

confidence: 99%

Increased Local Testosterone Levels Alter Human Fallopian Tube mRNA Profile and Signaling

Russo

Cain

Jackson-Bey

et al. 2023

Cancers

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Fallopian tube epithelium (FTE) plays a critical role in reproduction and can be the site where High Grade Serous Ovarian Carcinoma (HGSOC) originates. Tumorigenic oviductal cells, which are the murine equivalent of human fallopian tube secretory epithelial cells (FTSEC), enhance testosterone secretion by the ovary when co-cultured with the ovary, suggesting that testosterone is part of the signaling axis between the ovary and FTSEC. Furthermore, testosterone promotes proliferation of oviductal cells. Oral contraceptives, tubal ligation, and salpingectomy, which are all protective against developing ovarian cancer, also decrease circulating levels of androgen. In the current study, we investigated the effect of increased testosterone on FTE and found that testosterone upregulates wingless-type MMTV integration family, member 4 (WNT4) and induces migration and invasion of immortalized human fallopian tube cells. We profiled primary human fallopian tissues grown in the microfluidic system SOLO-microfluidic platform –(MFP) by RNA sequencing and found that p53 and its downstream target genes, such as paired box gene 2 (PAX2), cyclin-dependent kinase inhibitor 1A (CDK1A or p21), and cluster of differentiation 82 (CD82 or KAI1) were downregulated in response to testosterone treatment. A microfluidic platform, the PREDICT-Multi Organ System (PREDICT-MOS) was engineered to support insert technology that allowed for the study of cancer cell migration and invasion through Matrigel. Using this system, we found that testosterone enhanced FTE migration and invasion, which was reversed by the androgen receptor (AR) antagonist, bicalutamide. Testosterone also enhanced FTSEC adhesion to the ovarian stroma using murine ovaries. Overall, these results indicate that primary human fallopian tube tissue and immortalized FTSEC respond to testosterone to shift expression of genes that regulate invasion, while leveraging a new strategy to study migration in the presence of dynamic fluid flow.

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Section: Discussionmentioning

confidence: 99%

Increased Local Testosterone Levels Alter Human Fallopian Tube mRNA Profile and Signaling

Russo

Cain

Jackson-Bey

et al. 2023

Cancers

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ARL13B controls male reproductive tract physiology through primary and Motile Cilia

Augière,

Campolina-Silva,

Vijayakumaran

et al. 2024

Commun Biol

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ARL13B is a small regulatory GTPase that controls ciliary membrane composition in both motile cilia and non-motile primary cilia. In this study, we investigated the role of ARL13B in the efferent ductules, tubules of the male reproductive tract essential to male fertility in which primary and motile cilia co-exist. We used a genetically engineered mouse model to delete Arl13b in efferent ductule epithelial cells, resulting in compromised primary and motile cilia architecture and functions. This deletion led to disturbances in reabsorptive/secretory processes and triggered an inflammatory response. The observed male reproductive phenotype showed significant variability linked to partial infertility, highlighting the importance of ARL13B in maintaining a proper physiological balance in these small ducts. These results emphasize the dual role of both motile and primary cilia functions in regulating efferent duct homeostasis, offering deeper insights into how cilia related diseases affect the male reproductive system.

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The Synergic Effect of Tubal Endometriosis and Women’s Aging on Fallopian Tube Function: Insights from a 3D Mechanical Model

Nassir,

Levi,

Shaked

2024

Bioengineering

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The fallopian tubes are essential for human fertility, facilitating the movement of sperm and oocytes to the fertilization site and transporting fertilized oocytes to the uterus. Infertility can result from changes in the fallopian tubes due to tubal endometriosis and women’s aging. In this study, we modeled human fallopian tubes with and without endometriosis for different women’s age groups to evaluate the chances of normal sperm cells reaching the fertilization site and oocytes arriving at the uterine cavity. For this purpose, we employed a distinctive combination of simulation tools to develop a dynamic three-dimensional (3D) model of normal human sperm cells and oocytes swimming inside normal and endometriosis-affected human fallopian tubes for different women’s group ages. We observed that in tubal endometriosis cases, fewer sperm cells reach the fertilization site and more oocytes become trapped in the tube walls compared to normal tubes. Additionally, aging decreases the number of sperm cells and oocytes reaching the fertilization site in normal and endometriosis-affected tubes. Our model evaluates the mechanisms of sperm and oocyte behaviors due to women’s aging and fallopian tube issues caused by endometriosis, presenting new avenues for developing diagnostic and treatment tools for tubal endometriosis and age-related infertility issues.

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Isolation of Fallopian Tube Epithelium for Assessment of Cilia Beating Frequency (CBF)

Cited by 3 publications

References 20 publications

Increased Local Testosterone Levels Alter Human Fallopian Tube mRNA Profile and Signaling

Increased Local Testosterone Levels Alter Human Fallopian Tube mRNA Profile and Signaling

ARL13B controls male reproductive tract physiology through primary and Motile Cilia

The Synergic Effect of Tubal Endometriosis and Women’s Aging on Fallopian Tube Function: Insights from a 3D Mechanical Model

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